Gene cloning: [principles and applications]
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Taylor & Francis
2007
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IX, 462 S. Ill., graph. Darst. |
| ISBN: | 0748765344 9780748765348 |
Internformat
MARC
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| 100 | 1 | |a Lodge, Julia |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Gene cloning |b [principles and applications] |c Julia Lodge, Pete Lund & Steve Minchin |
| 264 | 1 | |a New York, NY [u.a.] |b Taylor & Francis |c 2007 | |
| 300 | |a IX, 462 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 4 | |a Clonage moléculaire | |
| 650 | 4 | |a Cloning, Molecular | |
| 650 | 4 | |a Gene Library | |
| 650 | 4 | |a Genomics |x methods | |
| 650 | 4 | |a Molecular cloning | |
| 650 | 0 | 7 | |a Genklonierung |0 (DE-588)4123275-6 |2 gnd |9 rswk-swf |
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| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Lund, Pete |e Verfasser |4 aut | |
| 700 | 1 | |a Minchin, Steve |e Verfasser |4 aut | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014604701&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-014604701 | ||
Datensatz im Suchindex
| _version_ | 1804135071621316608 |
|---|---|
| adam_text | Contents
Chapter I Introduction
Chapter
Chapter
1.1
The Beginning of Gene Cloning
1
1.2
How To Use This Book
3
1.3
What You Need To Know Before You Read This Book
5
1.4
A Request From the Authors
5
Further Reading
6
Genome Organization
2.1
Introduction
7
2.2
The C-value Paradox
8
2.3
The Human Genome
9
2.4
Genomes of Other Eukaryotes
19
2.5
Bacterial Genomes
24
2.6
Plasmids
25
2.7
Viral Genomes
26
2.8
GC Content
27
2.9
Physical Characteristics of Eukaryotic Chromosomes
28
2.10
Karyotype
28
2.11
Euchromatin and Heterochromatin
30
2.12
CpG Islands
31
Questions and Answers
32
Further Reading
34
Key Tools for Gene Cloning
3.1
Introduction
35
3.2
Vectors
36
3.3
Restriction Enzymes
38
3.4
DNA
40
3.5
Transformation
42
3.6
Purification of Plasmid
45
3.7
More Restriction Enzymes
47
3.8
Alkaline Phosphatase
51
3.9
More About Vectors
53
3.10
Analyzing Cloned
58
3.11
Measuring the Size of
59
3.12
The Polymerase Chain Reaction and Its Use in Gene Cloning
64
3.13
How Does PCR Work?
67
vi
3.14
3.15
3.16
3.17
3.18
3.19
Questions and Answers
Further Reading 83
Chapter
4.1
4.2
4.3
4.4
4.5
Libraries
4.6
4.7
4.8
4.9
4.10
4.11
4.12
4.13
4.14
4.15
4.16
4.17
4.18
4.19
4.20
4.21
Questions and Answers
Further Reading
Chapter
5.1
5.2
5.3
5.4
5.5
5.6
5.7
Contents
5.8
5.9
5.10
Questions and Answers
Further Reading
127
132
133
135
140
Chapter
6.1
Problem in Gene Cloning
6.2
6.3
Adherence Genes from Pseudomonas
6.4
Difficult Phenotypes
6.5
Plants
6.6
6.7
6.8
6.9
6.10
Questions and Answers
Further Reading
Chapter
7.1
7.2
7.3 Sänger
7.4
DNA
7.5
7.6
7.7
7.8
7.9
7.10
7.11
Questions and Answers
Further Reading
Chapter
8.1
8.2
viii Contents
8.3
8.4
8.5
8.6
8.7
8.8
Sequences Being Compared?
8.9
8.10
8.11
8.12
the Sequences Being Compared?
8.13
8.14
Structure and Function of Proteins?
8.15
8.16
Molecules
8.17
Questions and Answers
Further Reading
Chapter
9.1
9.2
9.3
9.4
Proteins in
9.5
9.6
Questions and Answers
Further Reading
Chapter 1
10.1
10.2
Determining the Cellular Location of Proteins
Mapping of Membrane Proteins
Detecting Interacting Proteins
Site-Directed Mutagenesis for Detailed Probing of Gene and
Protein Function
Questions and Answers
Further Reading
10.3
10.4
10.5
10.6
Contents ix
Chapter 11 The Analysis of the Regulation of Gene Expression
11.1
11.2
11.3
11.4
11.5
11.6
Questions and Answers
Further Reading
Chapter 1
12.1
12.2
12.3
12.4
12.5
Precision in
12.6
Questions and Answers
Further Reading
Chapter
13.1
13.2
13.3 DNA
13.4
13.5
13.6
13.7
13.8
13.9
13.10
13.11
13.12
Questions and Answers
Further Reading
Glossary
Index
315
318
326
338
350
353
361
364
365
367
377
396
398
406
407
410
411
411
413
414
415
416
417
418
422
435
437
439
441
444
445
453
|
| adam_txt |
Contents
Chapter I Introduction
Chapter
Chapter
1.1
The Beginning of Gene Cloning
1
1.2
How To Use This Book
3
1.3
What You Need To Know Before You Read This Book
5
1.4
A Request From the Authors
5
Further Reading
6
Genome Organization
2.1
Introduction
7
2.2
The C-value Paradox
8
2.3
The Human Genome
9
2.4
Genomes of Other Eukaryotes
19
2.5
Bacterial Genomes
24
2.6
Plasmids
25
2.7
Viral Genomes
26
2.8
GC Content
27
2.9
Physical Characteristics of Eukaryotic Chromosomes
28
2.10
Karyotype
28
2.11
Euchromatin and Heterochromatin
30
2.12
CpG Islands
31
Questions and Answers
32
Further Reading
34
Key Tools for Gene Cloning
3.1
Introduction
35
3.2
Vectors
36
3.3
Restriction Enzymes
38
3.4
DNA
40
3.5
Transformation
42
3.6
Purification of Plasmid
45
3.7
More Restriction Enzymes
47
3.8
Alkaline Phosphatase
51
3.9
More About Vectors
53
3.10
Analyzing Cloned
58
3.11
Measuring the Size of
59
3.12
The Polymerase Chain Reaction and Its Use in Gene Cloning
64
3.13
How Does PCR Work?
67
vi
3.14
3.15
3.16
3.17
3.18
3.19
Questions and Answers
Further Reading 83
Chapter
4.1
4.2
4.3
4.4
4.5
Libraries
4.6
4.7
4.8
4.9
4.10
4.11
4.12
4.13
4.14
4.15
4.16
4.17
4.18
4.19
4.20
4.21
Questions and Answers
Further Reading
Chapter
5.1
5.2
5.3
5.4
5.5
5.6
5.7
Contents
5.8
5.9
5.10
Questions and Answers
Further Reading
127
132
133
135
140
Chapter
6.1
Problem in Gene Cloning
6.2
6.3
Adherence Genes from Pseudomonas
6.4
"Difficult" Phenotypes
6.5
Plants
6.6
6.7
6.8
6.9
6.10
Questions and Answers
Further Reading
Chapter
7.1
7.2
7.3 Sänger
7.4
DNA
7.5
7.6
7.7
7.8
7.9
7.10
7.11
Questions and Answers
Further Reading
Chapter
8.1
8.2
viii Contents
8.3
8.4
8.5
8.6
8.7
8.8
Sequences Being Compared?
8.9
8.10
8.11
8.12
the Sequences Being Compared?
8.13
8.14
Structure and Function of Proteins?
8.15
8.16
Molecules
8.17
Questions and Answers
Further Reading
Chapter
9.1
9.2
9.3
9.4
Proteins in
9.5
9.6
Questions and Answers
Further Reading
Chapter 1
10.1
10.2
Determining the Cellular Location of Proteins
Mapping of Membrane Proteins
Detecting Interacting Proteins
Site-Directed Mutagenesis for Detailed Probing of Gene and
Protein Function
Questions and Answers
Further Reading
10.3
10.4
10.5
10.6
Contents ix
Chapter 11 The Analysis of the Regulation of Gene Expression
11.1
11.2
11.3
11.4
11.5
11.6
Questions and Answers
Further Reading
Chapter 1
12.1
12.2
12.3
12.4
12.5
Precision in
12.6
Questions and Answers
Further Reading
Chapter
13.1
13.2
13.3 DNA
13.4
13.5
13.6
13.7
13.8
13.9
13.10
13.11
13.12
Questions and Answers
Further Reading
Glossary
Index
315
318
326
338
350
353
361
364
365
367
377
396
398
406
407
410
411
411
413
414
415
416
417
418
422
435
437
439
441
444
445
453 |
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| illustrated | Illustrated |
| index_date | 2024-07-02T13:47:54Z |
| indexdate | 2024-07-09T20:34:41Z |
| institution | BVB |
| isbn | 0748765344 9780748765348 |
| language | English |
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| spelling | Lodge, Julia Verfasser aut Gene cloning [principles and applications] Julia Lodge, Pete Lund & Steve Minchin New York, NY [u.a.] Taylor & Francis 2007 IX, 462 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Clonage moléculaire Cloning, Molecular Gene Library Genomics methods Molecular cloning Genklonierung (DE-588)4123275-6 gnd rswk-swf Genklonierung (DE-588)4123275-6 s b DE-604 Lund, Pete Verfasser aut Minchin, Steve Verfasser aut Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014604701&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lodge, Julia Lund, Pete Minchin, Steve Gene cloning [principles and applications] Clonage moléculaire Cloning, Molecular Gene Library Genomics methods Molecular cloning Genklonierung (DE-588)4123275-6 gnd |
| subject_GND | (DE-588)4123275-6 |
| title | Gene cloning [principles and applications] |
| title_auth | Gene cloning [principles and applications] |
| title_exact_search | Gene cloning [principles and applications] |
| title_exact_search_txtP | Gene cloning [principles and applications] |
| title_full | Gene cloning [principles and applications] Julia Lodge, Pete Lund & Steve Minchin |
| title_fullStr | Gene cloning [principles and applications] Julia Lodge, Pete Lund & Steve Minchin |
| title_full_unstemmed | Gene cloning [principles and applications] Julia Lodge, Pete Lund & Steve Minchin |
| title_short | Gene cloning |
| title_sort | gene cloning principles and applications |
| title_sub | [principles and applications] |
| topic | Clonage moléculaire Cloning, Molecular Gene Library Genomics methods Molecular cloning Genklonierung (DE-588)4123275-6 gnd |
| topic_facet | Clonage moléculaire Cloning, Molecular Gene Library Genomics methods Molecular cloning Genklonierung |
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