Bioengineering at the micro-scale :: design, characterization and validation of microbioreactors /
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Göttingen :
Cuvillier Verlag,
2019.
|
| Schriftenreihe: | Schriftenreihe des Institutes für Bioverfahrenstechnik der Technischen Universität Braunschweig.
|
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Beschreibung: | "Bei der Fakultät für Maschinenbau der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung der Würde einer Doktor-Ingenieurin (Dr.-Ing.) eingereichte Dissertation." |
| Beschreibung: | 1 online resource (139 pages) |
| Bibliographie: | Includes bibliographical references. |
| ISBN: | 9783736960251 3736960255 |
Internformat
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| 245 | 1 | 0 | |a Bioengineering at the micro-scale : |b design, characterization and validation of microbioreactors / |c Susanna Maria Lladó Maldonado. |
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| 500 | |a "Bei der Fakultät für Maschinenbau der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung der Würde einer Doktor-Ingenieurin (Dr.-Ing.) eingereichte Dissertation." | ||
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| 650 | 0 | |a Biotechnology. |0 http://id.loc.gov/authorities/subjects/sh85014263 | |
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| 880 | |6 520-00/(S |a The miniaturisation of bioreactors to the microliter-scale and the integration of online sensors for monitoring the most important process variables during the cultivations is a promising approach for the screening and optimization of cultivation and biocatalysis processes, enabling information-rich, parallelised and cost-effective experiments under well-controlled environmental conditions. The main advantages of microbioreactors (MBRs) are minimization of space and reagents, their easy manipulation, and their high-throughput screening potential, which make them very interesting tools to develop bioprocesses. This thesis is focused on the development of MBRs and the sensor integration for monitoring optical density (OD), dissolved oxygen (DO), pH and glucose as well as their validation for different biotechnological applications. On the basis of a developed PDMS-based MBR, a borosilicate glass-based microbubble column-biroeactor (gμBC) (working volume of 60 μL, aeration occurs through a nozzle with Ø =26 μm) was designed and manufactured by wet etching and powder blasting technology. The gµBC proved to have good oxygen transfer capacity, reaching kLa values up to 320 1/h and fast mixing times θ95 down to 5.5 s when working at a gas superficial velocity uG of 2.25∙10-3 m/s. The mixing performance was simulated using a simplified CFD model, and the tracer profile yielded a good qualitative prediction that was comparable to the experimental results, presenting a tolerable deviation of the mixing times. The gµBC was validated as a suitable cultivation screening tool with a batch cultivation of Saccharomyces cerevisiae with the real-time online monitoring of OD and DO. Furthermore, a cuvette-based microbubble column-bioreactor (cμBC) made of polystyrene (working volume of 550 μL, aeration occurs through a nozzle with Ø ≤ 100 μm) was developed and manufactured with online sensors for pH, OD, DO and glucose. The cμBC showed homogeneous mixing of the cultivation medium with θ95 < 1 s, with high kLa up to 775 1/h at uG of 8.4∙10 3 m/s. The applicability of the cμBC for aerobic submerged whole-cell cultivation in batch and chemostat mode was demonstrated with the model organisms S. cerevisiae and Staphylococcus carnosus. In addition, the use of the cμBC for oxygen-dependent (cell-free) biocatalysis was successfully demonstrated with the example of the model enzyme glucose oxidase immobilization on supports to convert glucose via gluconolactone and hydrogen peroxide to gluconic acid in a microfluidic bed bioreactor (μFBR). The characterization of the developed and manufactured MBRs in this work as well as the integration of the online sensors for OD, DO, pH and glucose are now the basis for the future development of a consolidated and parallelisable MBR system for bioprocess development. | ||
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| genre_facet | Academic Dissertation dissertations. Academic theses Academic theses. Thèses et écrits académiques. |
| id | ZDB-4-EBA-on1104080272 |
| illustrated | Not Illustrated |
| indexdate | 2024-11-27T13:29:30Z |
| institution | BVB |
| isbn | 9783736960251 3736960255 |
| language | English |
| oclc_num | 1104080272 |
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| physical | 1 online resource (139 pages) |
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| publishDate | 2019 |
| publishDateSearch | 2019 |
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| publisher | Cuvillier Verlag, |
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| series | Schriftenreihe des Institutes für Bioverfahrenstechnik der Technischen Universität Braunschweig. |
| series2 | Schriftenreihe des Institutes für Bioverfahrenstechnik der Technischen Universität Braunschweig ; |
| spelling | Lladó Maldonado, Susanna Maria, 1988- author. Bioengineering at the micro-scale : design, characterization and validation of microbioreactors / Susanna Maria Lladó Maldonado. Göttingen : Cuvillier Verlag, 2019. 1 online resource (139 pages) text txt rdacontent computer c rdamedia online resource cr rdacarrier Schriftenreihe des Institutes für Bioverfahrenstechnik der Technischen Universität Braunschweig ; Band 81 In English; abstract also in German. "Bei der Fakultät für Maschinenbau der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung der Würde einer Doktor-Ingenieurin (Dr.-Ing.) eingereichte Dissertation." Includes bibliographical references. Online resource; title from PDF title page (EBSCO, viewed June 3, 2021). Bioreactors. http://id.loc.gov/authorities/subjects/sh87005835 Bioengineering. http://id.loc.gov/authorities/subjects/sh85014134 Biotechnology. http://id.loc.gov/authorities/subjects/sh85014263 Bioreactors https://id.nlm.nih.gov/mesh/D019149 Biotechnology https://id.nlm.nih.gov/mesh/D001709 Bioréacteurs. Biotechnologie. bioengineering. aat Bioengineering fast Bioreactors fast Biotechnology fast Academic Dissertation https://id.nlm.nih.gov/mesh/D019478 dissertations. aat Academic theses fast Academic theses. lcgft http://id.loc.gov/authorities/genreForms/gf2014026039 Thèses et écrits académiques. rvmgf has work: Bioengineering at the micro-scale (Text) https://id.oclc.org/worldcat/entity/E39PCGJb9FRGWqqfjCtfbjbV3P https://id.oclc.org/worldcat/ontology/hasWork Print version: 9783736970250 Schriftenreihe des Institutes für Bioverfahrenstechnik der Technischen Universität Braunschweig. FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=2147472 Volltext 520-00/(S The miniaturisation of bioreactors to the microliter-scale and the integration of online sensors for monitoring the most important process variables during the cultivations is a promising approach for the screening and optimization of cultivation and biocatalysis processes, enabling information-rich, parallelised and cost-effective experiments under well-controlled environmental conditions. The main advantages of microbioreactors (MBRs) are minimization of space and reagents, their easy manipulation, and their high-throughput screening potential, which make them very interesting tools to develop bioprocesses. This thesis is focused on the development of MBRs and the sensor integration for monitoring optical density (OD), dissolved oxygen (DO), pH and glucose as well as their validation for different biotechnological applications. On the basis of a developed PDMS-based MBR, a borosilicate glass-based microbubble column-biroeactor (gμBC) (working volume of 60 μL, aeration occurs through a nozzle with Ø =26 μm) was designed and manufactured by wet etching and powder blasting technology. The gµBC proved to have good oxygen transfer capacity, reaching kLa values up to 320 1/h and fast mixing times θ95 down to 5.5 s when working at a gas superficial velocity uG of 2.25∙10-3 m/s. The mixing performance was simulated using a simplified CFD model, and the tracer profile yielded a good qualitative prediction that was comparable to the experimental results, presenting a tolerable deviation of the mixing times. The gµBC was validated as a suitable cultivation screening tool with a batch cultivation of Saccharomyces cerevisiae with the real-time online monitoring of OD and DO. Furthermore, a cuvette-based microbubble column-bioreactor (cμBC) made of polystyrene (working volume of 550 μL, aeration occurs through a nozzle with Ø ≤ 100 μm) was developed and manufactured with online sensors for pH, OD, DO and glucose. The cμBC showed homogeneous mixing of the cultivation medium with θ95 < 1 s, with high kLa up to 775 1/h at uG of 8.4∙10 3 m/s. The applicability of the cμBC for aerobic submerged whole-cell cultivation in batch and chemostat mode was demonstrated with the model organisms S. cerevisiae and Staphylococcus carnosus. In addition, the use of the cμBC for oxygen-dependent (cell-free) biocatalysis was successfully demonstrated with the example of the model enzyme glucose oxidase immobilization on supports to convert glucose via gluconolactone and hydrogen peroxide to gluconic acid in a microfluidic bed bioreactor (μFBR). The characterization of the developed and manufactured MBRs in this work as well as the integration of the online sensors for OD, DO, pH and glucose are now the basis for the future development of a consolidated and parallelisable MBR system for bioprocess development. |
| spellingShingle | Lladó Maldonado, Susanna Maria, 1988- Bioengineering at the micro-scale : design, characterization and validation of microbioreactors / Schriftenreihe des Institutes für Bioverfahrenstechnik der Technischen Universität Braunschweig. Bioreactors. http://id.loc.gov/authorities/subjects/sh87005835 Bioengineering. http://id.loc.gov/authorities/subjects/sh85014134 Biotechnology. http://id.loc.gov/authorities/subjects/sh85014263 Bioreactors https://id.nlm.nih.gov/mesh/D019149 Biotechnology https://id.nlm.nih.gov/mesh/D001709 Bioréacteurs. Biotechnologie. bioengineering. aat Bioengineering fast Bioreactors fast Biotechnology fast |
| subject_GND | http://id.loc.gov/authorities/subjects/sh87005835 http://id.loc.gov/authorities/subjects/sh85014134 http://id.loc.gov/authorities/subjects/sh85014263 https://id.nlm.nih.gov/mesh/D019149 https://id.nlm.nih.gov/mesh/D001709 https://id.nlm.nih.gov/mesh/D019478 http://id.loc.gov/authorities/genreForms/gf2014026039 |
| title | Bioengineering at the micro-scale : design, characterization and validation of microbioreactors / |
| title_auth | Bioengineering at the micro-scale : design, characterization and validation of microbioreactors / |
| title_exact_search | Bioengineering at the micro-scale : design, characterization and validation of microbioreactors / |
| title_full | Bioengineering at the micro-scale : design, characterization and validation of microbioreactors / Susanna Maria Lladó Maldonado. |
| title_fullStr | Bioengineering at the micro-scale : design, characterization and validation of microbioreactors / Susanna Maria Lladó Maldonado. |
| title_full_unstemmed | Bioengineering at the micro-scale : design, characterization and validation of microbioreactors / Susanna Maria Lladó Maldonado. |
| title_short | Bioengineering at the micro-scale : |
| title_sort | bioengineering at the micro scale design characterization and validation of microbioreactors |
| title_sub | design, characterization and validation of microbioreactors / |
| topic | Bioreactors. http://id.loc.gov/authorities/subjects/sh87005835 Bioengineering. http://id.loc.gov/authorities/subjects/sh85014134 Biotechnology. http://id.loc.gov/authorities/subjects/sh85014263 Bioreactors https://id.nlm.nih.gov/mesh/D019149 Biotechnology https://id.nlm.nih.gov/mesh/D001709 Bioréacteurs. Biotechnologie. bioengineering. aat Bioengineering fast Bioreactors fast Biotechnology fast |
| topic_facet | Bioreactors. Bioengineering. Biotechnology. Bioreactors Biotechnology Bioréacteurs. Biotechnologie. bioengineering. Bioengineering Academic Dissertation dissertations. Academic theses Academic theses. Thèses et écrits académiques. |
| url | https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=2147472 |
| work_keys_str_mv | AT lladomaldonadosusannamaria bioengineeringatthemicroscaledesigncharacterizationandvalidationofmicrobioreactors |