Verfügbarkeit: Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major

Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major: = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major

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Bibliographische Detailangaben
1. Verfasser:	Leitherer, Sabine (VerfasserIn)
Format:	Abschlussarbeit Buch
Sprache:	English
Veröffentlicht:	Erlangen ; Nürnberg 2017
Schlagworte:	Immunreaktion Genexpression Proteintyrosinphosphatase Leishmania major Hochschulschrift
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	161 Seiten Illustrationen, Diagramme

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MARC


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246	1	1	\|a Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major
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Datensatz im Suchindex

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adam_text	TABLE OF CONTENT Z U S AM M E N FA SS U N G ..............................................................................................................................................................................-1 S U M M ARY ..................................................................................................................................................................................................- 3 1 IN TRO D U C TIO N .......................................................................................................................................................................................... - 5 1.1 LEISHMANIA DISEASE AND PARASITE......................................................................................................................... ...............- 5 1.1.1 EPIDEMIOLOGY OF LEISHMANIASIS.................................................................................................................................. - 5 1.1.1 LIFE CYCLE OF LEISHMANIA PARASITES...........................................................................................................................- 8 1.2 SURFACE COAT OF LEISHMANIA AND MECHANISM OF PROTEIN EXPORT..................................................................................-1 1 1.2.1 SURFACE COAT...................................................................................................................................................................-1 1 1.2.2 SECRETORY PATHWAY IN LEISHM ANIA...........................................................................................................................- 1 3 1.3 IMMUNE RESPONSE DURING LEISHMANIA INFECTION............................................................................................................. - 1 5 1.3.1 INNATE IMMUNE RESPONSE DURING LEISHMANIA INFECTION................................................................................... - 1 5 1.3.2 ADAPTIVE IMMUNE RESPONSE DURING LEISHMANIA INFECTION: HOST RESISTANCE AND SUSCEPTIBILITY.............- IS 1.4 IMMUNE EVASION STRATEGIES OF LEISHMANIA PARASITES................................................................................................... - 1 9 1.4.1 INTERFERENCE WITH HOST CELL SIGNALING......................................................................................................................- 1 9 1.4.2 VIRULENCE FACTORS...........................................................................................................................................................-2 1 1.5 SEARCH FOR PUTATIVE VIRULENCE FACTORS OF LEISHMANIA: DESIGN OF THE PROJECT .......................................................... - 23 1.5.1 PHOSPHATASES AS VIRULENCE FACTORS IN LEISHM ANIA............................................................................................- 2 3 1.5.2 THE PHOSPHATASES OF REGENERATING L IVER............................................................................................................- 2 6 2 S CIENTIFIC QUESTION, W O RKIN G HYPOTHESIS AND A IM S ...........................................................................................................- 29 3 M ATERIAL AND M E TH O D S ..................................................................................................................................................................... - 31 3.1 M ATERIAL...................................................................................................................................................................................... -3 1 3.2 MOLECULAR BIOLOGY METHODS...................................................................................................................................................- 39 3.3 BIOCHEMISTRY METHODS........................................................................................................................................................... - 4 7 3.4 CELL CULTURE M ETHODS..............................................................................................................................................................- 51 3.5 FLUORESCENCE MICROSCOPY AND F A C S ...............................................................................................................................- 59 3.6 ANIMAL EXPERIMENTS................................................................................................................................................................ - 61 3.7 STATISTICAL ANALYSIS...................................................................................................................................................................- 64 4 R E S U LTS ..................................................................................................................................................................................................- 65 4.1 STRUCTURE AND BIOCHEMICAL CHARACTERIZATION OF LM P R L ............................................................................................... - 6 5 4.1.1 ANALYSIS OF THE SEQUENCE AND STRUCTURE OF LMPRL PHOSPHATASES .............................................................. - 6 5 4.1.2 BIOCHEMICAL CHARACTERIZATION OF THE LMPRL PHOSPHATASE ACTIVITY................................................................- 69 4.2 FUNCTIONAL CHARACTERIZATION OF LMPRL IN L M AJOR PARASITES.....................................................................................- 73 4.2.1 CHARACTERIZATION OF THE PROTEIN EXPRESSION PROFILE OF LMPRL IN L M AJOR PARASITES................................- 73 4.2.2 INFLUENCE OF LMPRL ON THE REPLICATION OF L M AJOR PROMASTIGOTES................................................................- 78 4 .2 .3 CHARACTERIZATION OF THE SUBCELLULAR LOCALIZATION OF LMPRL IN L M AJOR PROMASTIGOTES ........................... - 8 4 - 4 .2.4 CHARACTERIZATION OF THE SECRETION OF LMPRL BY L M AJOR PROMASTIGOTES....................................................- 8 6 - 4 .3 FUNCTIONAL CHARACTERIZATION OF LMPRL DURING MACROPHAGE INFECTION IN V ITRO .......................................................... - 8 9 - 4.3.1 EXPRESSION OF LMPRL DURING IN VITRO INFECTION OF M ACROPHAGES...................................................................- 89 - 4.3.2 SUBCELLULAR LOCALIZATION OF HAS-LMPRL DURING MACROPHAGE INFECTION WITH L M AJOR PROMASTIGOTES ..-9 1 - 4 .3.3 INFLUENCE OF HA3-LMPRL OVER-EXPRESSION BY L. M AJOR DURING INFECTION OF M ACROPHAGES.....................- 9 5 - 4 .3 .4 IMPACT OF LMPRL DELETION IN L M AJOR PARASITES DURING MACROPHAGE INFECTION ....................................... - 98 - 4.4 CHARACTERIZATION OF THE VIRULENCE OF THE LMPRL-1 DELETED L M AJOR STRAIN IN A MURINE MODEL OF CUTANEOUS LEISHM ANIASIS.......................................................................................................................................................................................... - 1 0 3 - 4.4.1 ROLE OF LMPRL-1 IN A MURINE MODEL OF HOST SUSCEPTIBILITY TO CUTANEOUS LEISHMANIASIS ...................... -1 0 4 - 4 .4.2 ROLE OF LMPRL-1 IN A MURINE MODEL OF HOST RESISTANCE TO CUTANEOUS LEISHMANIASIS ........................... - 1 0 6 - 4 .5 FUNCTIONAL STUDY OF LMPRL IN THE CONTEXT OF MACROPHAGE CELL SIGNALING ............................................................ -1 0 8 - 4.5.1 ANALYSIS OF A PUTATIVE INTERACTION BETWEEN LMPRL AND PRL PROTEINS IN M ACROPHAGES ...................... -1 0 8 - 4.5.2 ANALYSIS OF THE INFLUENCE OF LMPRL ON ACTIN CYTOSKELETON DYNAMICS IN MACROPHAGE CELL LINES .......... -1 1 9 - 4 .5 .3 INFLUENCE OF LMPRL ON M APK SIGNALING IN M ACROPHAGES.............................................................................-1 2 5 - 5 D IS C U S S IO N ..........................................................................................................................................................................................- 1 2 9 - 5.1 BIOCHEMICAL AND STRUCTURAL ANALOGY BETWEEN LMPRL AND HUMAN P R L ..............................................................-1 2 9 - 5.2 EXPRESSION OF LMPRL DURING THE LEISHMANIA LIFE CYCLE............................................................................................-1 3 1 - 5.3 SECRETION AND SUBCELLULAR LOCALIZATION OF LM PRL........................................................................................................ - 1 3 4 - 5.4 PRO-SURVIVAL EFFECT OF LMPRL ON L M AJOR DURING INFECTION.......................................................................................- 1 3 6 - 5.5 ALTERATION OF MACROPHAGE FUNCTION DUE TO POSSIBLE INTERACTION OF LMPRL WITH HOST P R L ..............................- 1 3 9 - 5.6 ROLE OF LMPRL IN SUBVERSION OF HOST CELL SIGNALING PATHWAYS BY LEISHMANIA PARASITES ............................... -1 4 1 - 5.7 MODEL OF THE ROLE OF LMPRL PHOSPHATASES DURING THE LIFE CYCLE OF LEISHMANIA PARASITES ............................ -1 4 3 - 6 C O N C LUDING RE M A RK S .......................................................................................................................................................................- 1 4 5 - 7 R E FE REN C E S ......................................................................................................................................................................................... - 147 - 8 A B B RE V IA TIO N S ................................................................................................................................................................................... -1 5 7 - 9 A P P E N D IX ............................................................................................................................................................................................. - 1 5 9 - 10 A C K N O W LE D G E M E N T........................................................................... - 1 6 1 -
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spelling	Leitherer, Sabine Verfasser aut Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major vorgelegt von Sabine Leitherer aus Bamberg Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major Erlangen ; Nürnberg 2017 161 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Friedrich-Alexander-Universität Erlangen-Nürnberg 2017 Immunreaktion (DE-588)4133841-8 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf Proteintyrosinphosphatase (DE-588)4311372-2 gnd rswk-swf Leishmania major (DE-588)4404287-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Leishmania major (DE-588)4404287-5 s Genexpression (DE-588)4020136-3 s Proteintyrosinphosphatase (DE-588)4311372-2 s Immunreaktion (DE-588)4133841-8 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029947180&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Leitherer, Sabine Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major Immunreaktion (DE-588)4133841-8 gnd Genexpression (DE-588)4020136-3 gnd Proteintyrosinphosphatase (DE-588)4311372-2 gnd Leishmania major (DE-588)4404287-5 gnd
subject_GND	(DE-588)4133841-8 (DE-588)4020136-3 (DE-588)4311372-2 (DE-588)4404287-5 (DE-588)4113937-9
title	Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major
title_alt	Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major
title_auth	Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major
title_exact_search	Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major
title_full	Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major vorgelegt von Sabine Leitherer aus Bamberg
title_fullStr	Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major vorgelegt von Sabine Leitherer aus Bamberg
title_full_unstemmed	Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major vorgelegt von Sabine Leitherer aus Bamberg
title_short	Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major
title_sort	biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite leishmania major biochemische und funktionelle charakterisierung zweier tyrosin phosphatasen des intrazellularen parasiten leishmania major
title_sub	= Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major
topic	Immunreaktion (DE-588)4133841-8 gnd Genexpression (DE-588)4020136-3 gnd Proteintyrosinphosphatase (DE-588)4311372-2 gnd Leishmania major (DE-588)4404287-5 gnd
topic_facet	Immunreaktion Genexpression Proteintyrosinphosphatase Leishmania major Hochschulschrift
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029947180&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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