A general model that explains the complex pattern of biallelic APC mutations in colorectal carcinoma, duodenal and desmoid tumours:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
[2009]
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| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-12318 http://d-nb.info/992510724/34 Inhaltsverzeichnis |
| Beschreibung: | IV, 82 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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| adam_text | Titel: A general model that explains the complex pattern of biallelic APC mutations in colorectal carcinoma
Autor: Kohler, Eva Maria
Jahr: 2009
Index
1. SUMMARY 1
2. ZUSAMMENFASSUNG 2
3. INTRODUCTION 3
3.1 THE ADENOMA-CARCINOMA SEQUENCE IN COLORECTAL CANCER 3
3.2 The tumour suppressor APC 3
3.2.1 Gene and protein structure of APC 3
3.2.2 APC in the canonical Wnt signalling pathway 5
3.2.3 Mutations in the Wnt pathway 7
3.3 Aim OF this work 10
4. RESULTS 11
4.1 ANALYSISOF THE BINDING CAPACITY OF B ATENIN TO APC 11
4.1.1 Introduction of mutations in the (i-catenin binding regions of APC 12
4.1.2 Investigation of the binding capacity ofthe 20R2 to fi-catenin 14
4.1.3 Investigation of the binding capacity of the 20R3 to (i-catenin 16
4.2 ANALYSISOF THE DEGRADATION CAPACITY OF TRUNCATED APC 20
4.2.2 (i-Catenin degrading APC truncations 20
4.2.2 Ammo acids 1404-1466 are critical for fi-catenin degradation 23
4.2.3 The activity of the CID is cell-specific 25
4.2.4 APC constructs lacking the CID interfere with (i-catenin degradation stimulated by constructs
containing it 27
4.2.5 Different APC constructs targeting (i-catenin for degradation are differentially inhibited by APC
constructs lacking the CID 29
4.2.6 The CID explains the mutation pattern found in tumours from FAP patients 29
4.2.7 The CID explains the mutation pattern found in sporadic colorectal tumours 33
5. DISCUSSION 34
5.1 THE 20R2 DOES NOT BIND TOB ATENIN 34
5.2 Functional definition of the MCR of APC in colorectal tumours 35
5.3 Identification and characterisation of a new domain of APC 37
5.4 Inhibition of the CID 39
5.5 a model for the selection of apc mutations 40
5.6 Further tasks 42
6. materials and methods 43
6.1 Materials 43
6.1.1 Technical Equipment 43
6.1.2 Chemicals 43
6.1.3 Enzymes 44
6.1.4 Kits 44
6.1.5 Buffers and media 44
6.1.6 Organisms 48
6.1.7 Antibodies 49
6.1.8 Oligonudeotides 50
6.1.9 Plasmids 52
6.2 Methods 53
6.2.1 Standard methods 53
6.2.2 Cloning and mulagenesis 53
6.2.3 Transient transfections 57
6.2.4 Co-immunoprecipitation and Western Blot 57
III
6.2.5 Immunoftuorescence stainings 58
6.2.6 Reporter assays 59
6.2.7 Yeast Two Hybrid screening 59
7. LITERATURE 61
8. ABBREVIATION INDEX 71
8.1 Abbreviations 71
8.2 Units 72
8.3 Dimensions 72
8.4 Amino acids 73
9. ANNEX 74
DNA-SequenceofAPC 74
APC constructs 76
Vector map mYF-APC constructs 78
Vector map mRF-fl-catenin 79
10. PUBLICATIONS 80
DANKSAGUNG 81
LEBENSLAUF 82
IV
|
| any_adam_object | 1 |
| author | Kohler, Eva Maria 1980- |
| author_GND | (DE-588)137113676 |
| author_facet | Kohler, Eva Maria 1980- |
| author_role | aut |
| author_sort | Kohler, Eva Maria 1980- |
| author_variant | e m k em emk |
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| bvnumber | BV035253992 |
| classification_rvk | XH 7204 |
| collection | ebook |
| ctrlnum | (OCoLC)314801151 (DE-599)DNB992510724 |
| dewey-full | 616.99434 571.97837 |
| dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
| dewey-ones | 616 - Diseases 571 - Physiology & related subjects |
| dewey-raw | 616.99434 571.97837 |
| dewey-search | 616.99434 571.97837 |
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| dewey-tens | 610 - Medicine and health 570 - Biology |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| spelling | Kohler, Eva Maria 1980- Verfasser (DE-588)137113676 aut A general model that explains the complex pattern of biallelic APC mutations in colorectal carcinoma, duodenal and desmoid tumours von Eva Maria Kohler [2009] IV, 82 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2008 Langzeitarchivierung Nationalbibliothek gewährleistet Catenine (DE-588)4380415-9 gnd rswk-swf Darmkrebs (DE-588)4011068-0 gnd rswk-swf Mutation (DE-588)4170883-0 gnd rswk-swf Protein C (DE-588)4123994-5 gnd rswk-swf Wnt-Proteine (DE-588)7515809-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Darmkrebs (DE-588)4011068-0 s Mutation (DE-588)4170883-0 s Wnt-Proteine (DE-588)7515809-7 s Protein C (DE-588)4123994-5 s Catenine (DE-588)4380415-9 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:29-opus-12318 https://open.fau.de/handle/openfau/777 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-12318 http://d-nb.info/992510724/34 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017059602&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kohler, Eva Maria 1980- A general model that explains the complex pattern of biallelic APC mutations in colorectal carcinoma, duodenal and desmoid tumours Catenine (DE-588)4380415-9 gnd Darmkrebs (DE-588)4011068-0 gnd Mutation (DE-588)4170883-0 gnd Protein C (DE-588)4123994-5 gnd Wnt-Proteine (DE-588)7515809-7 gnd |
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| title | A general model that explains the complex pattern of biallelic APC mutations in colorectal carcinoma, duodenal and desmoid tumours |
| title_auth | A general model that explains the complex pattern of biallelic APC mutations in colorectal carcinoma, duodenal and desmoid tumours |
| title_exact_search | A general model that explains the complex pattern of biallelic APC mutations in colorectal carcinoma, duodenal and desmoid tumours |
| title_full | A general model that explains the complex pattern of biallelic APC mutations in colorectal carcinoma, duodenal and desmoid tumours von Eva Maria Kohler |
| title_fullStr | A general model that explains the complex pattern of biallelic APC mutations in colorectal carcinoma, duodenal and desmoid tumours von Eva Maria Kohler |
| title_full_unstemmed | A general model that explains the complex pattern of biallelic APC mutations in colorectal carcinoma, duodenal and desmoid tumours von Eva Maria Kohler |
| title_short | A general model that explains the complex pattern of biallelic APC mutations in colorectal carcinoma, duodenal and desmoid tumours |
| title_sort | a general model that explains the complex pattern of biallelic apc mutations in colorectal carcinoma duodenal and desmoid tumours |
| topic | Catenine (DE-588)4380415-9 gnd Darmkrebs (DE-588)4011068-0 gnd Mutation (DE-588)4170883-0 gnd Protein C (DE-588)4123994-5 gnd Wnt-Proteine (DE-588)7515809-7 gnd |
| topic_facet | Catenine Darmkrebs Mutation Protein C Wnt-Proteine Hochschulschrift |
| url | https://open.fau.de/handle/openfau/777 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-12318 http://d-nb.info/992510724/34 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017059602&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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