Structural characterization of a type 1 secretion systems from Salmonella: = Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
Erlangen ; Nürnberg
[2018]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | xi, 126 Seiten Illustrationen, Diagramme 21 cm |
Internformat
MARC
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| 003 | DE-604 | ||
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| 100 | 1 | |a Kirchweger, Peter |e Verfasser |0 (DE-588)1175224162 |4 aut | |
| 245 | 1 | 0 | |a Structural characterization of a type 1 secretion systems from Salmonella |b = Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella |c vorgelegt von Peter Kirchweger |
| 246 | 1 | 3 | |a Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella |
| 246 | 1 | 1 | |a Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella |
| 264 | 1 | |a Erlangen ; Nürnberg |c [2018] | |
| 300 | |a xi, 126 Seiten |b Illustrationen, Diagramme |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Friedrich-Alexander Universität Erlangen-Nürnberg |d 2018 | ||
| 650 | 0 | 7 | |a Strukturaufklärung |0 (DE-588)4183788-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Sekretion |0 (DE-588)4124179-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Salmonella |0 (DE-588)4178963-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Proteine |0 (DE-588)4076388-2 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Salmonella |0 (DE-588)4178963-5 |D s |
| 689 | 0 | 1 | |a Sekretion |0 (DE-588)4124179-4 |D s |
| 689 | 0 | 2 | |a Proteine |0 (DE-588)4076388-2 |D s |
| 689 | 0 | 3 | |a Strukturaufklärung |0 (DE-588)4183788-5 |D s |
| 689 | 0 | |5 DE-604 | |
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| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030833303&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030833303 | ||
Datensatz im Suchindex
| _version_ | 1804179342631108608 |
|---|---|
| adam_text | CONTENTS
ABBREVIATIONS........................................................................................................................................
VII
ZUSAMMENFASSUNG...................................................................................................................................X
ABSTRACT....................................................................................................................................................XI
1
INTRODUCTION....................................................................................................................................1
1.1 SALMONELLA
ENTERICA.........................................................................................................
1
1.2 PATHOGENICITY
ISLANDS......................................................................................................
2
1.3 SPI4-T1SS AND ITS ROLE IN
PATHOGENICITY...........................................................................2
1.4
1188S...............................................................................................................................
4
1.4.1
ABCS..............................................................................................................................................................
4
1.4.2
PAPS...............................................................................................................................................................
5
1.4.3 O M
PS.............................................................................................................................................................6
1.4.4
SUBSTRATES.....................................................................................................................................................6
1.4.5 STATOR ELEM
ENTS..........................................................................................................................................
7
1.5 OVERVIEW ON STRUCTURAL BIOLOGY: MACROMOLECULAR X-RAY CRYSTALLOGRAPHY
AND CRYO-
ELECTRON
MICROSCOPY.....................................................................................................................
7
1.5.1
CRYSTALLOGRAPHY............................................................................................................................................8
1.5.2
CRYO-EM......................................................................................................................................................
10
1.5.3 RADIATION DAM AG
E....................................................................................................................................16
1.6 AIM OF THIS
STUDY...........................................................................................................
19
2
MATERIALS.......................................................................................................................................21
2.1 CHEMICALS, BUFFERS AND
MEDIA.......................................................................................21
2.2 MATERIALS FOR DNA MODIFICATION AND PROTEIN
PRODUCTION..............................................25
2.3 CONSUMABLES, DEVICES AND
EQUIPMENT.........................................................................27
3
METHODS........................................................................................................................................
31
3.1 POLYACRYLAMIDE GEL
ELECTROPHORESIS..............................................................................31
3.2 CHEMICAL TRANSFORMATION OF E.
COLI................................................................................31
3.3 PROTEIN
PURIFICATION........................................................................................................32
3.3.1 CONSTRUCTS USED IN THIS
STUDY................................................................................................................32
3.3.2 SIIA
PURIFICATION........................................................................................................................................
33
3.3.3 SIIAB
PURIFICATION......................................................................................................................................35
3.4 PROTEIN CONCENTRATION
MEASUREMENT..............................................................................38
3.5 CIRCULAR DICHROISM
SPECTROSCOPY....................................................................................38
3.6 PROTEIN
CRYSTALLIZATION....................................................................................................39
3.7 CRYSTALLOGRAPHIC DATA COLLECTION AND PROTEIN STRUCTURE SOLUTION
.................................
41
3.8 DEPICTION AND BIOINFORMATIC ANALYSIS OF THE
STRUCTURES................................................45
3.9 NEGATIVE STAIN
EM.........................................................................................................
46
3.9.1 SIIAB IN D D M
............................................................................................................................................
46
3.9.2 SIIAB IN DIB M A
.........................................................................................................................................46
3.10 MINICELL PRODUCTION AND SPECIMEN
PREPARATION...........................................................47
3.11 ELECTRON
TOMOGRAPHY....................................................................................................
48
3.12 SUBTOMOGRAM
AVERAGING..............................................................................................48
4
RESULTS...........................................................................................................
51
4.1
SIIA................................................................................................................................
51
4.1.1 PURIFICATION OF
SIIA...................................................................................................................................51
4.1.2 ANALYTICAL GEL
FILTRATION...........................................................................................................................55
4.1.3 CD
SPECTROSCOPY......................................................................................................................................
56
4.1.4 SIIA_TL CRYSTALLIZATION AND DATA
COLLECTION........................................................................................
57
4.1.5 CRYSTAL STRUCTURE OF
SIIA_TL...................................................................................................................59
4.1.6 SIIA IS A PG-BINDING
PROTEIN...................................................................................................................67
4.2
SIIAB..............................................................................................................................
70
4.2.1 SIIAB
PURIFICATION.....................................................................................................................................
70
4.2.2 CD
SPECTROSCOPY......................................................................................................................................
76
4.2.3 NEGATIVE STAIN E M
...................................................................................................................................76
4.3 SUBTOMOGRAM AVERAGING OF THE
SPI4-T1SS...................................................................77
4.3.1 CRYO-ET OF
SALMONELLA
MINICELLS...........................................................................................................77
4.3.2 SUBTOMOGRAM
AVERAGING.......................................................................................................................
81
5
DISCUSSION....................................................................................................................................
85
5.1 SIIAB
COMPLEX...............................................................................................................85
5.1.1 SIIA_TL PURIFICATION AND
CRYSTALLIZATION..............................................................................................85
5.1.2 LIGAND BINDING OF
SIIA.............................................................................................................................87
5.1.3 SIIA, A PG-BINDING
PROTEIN.....................................................................................................................88
5.1.4 SIIAB
PURIFICATION.....................................................................................................................................91
5.2 HOMOLOGY MODEL OF THE
SPI4-T1SS...............................................................................92
5.3 CRYO-ET OF SALMONELLA
MINICELLS....................................................................................94
5.4 SUBTOMOGRAM AVERAGING OF THE
SPI4-T1SS...................................................................96
5.5 MODEL OF SIIA
ACTIVATION................................................................................................97
5.6
CONCLUSIONS.................................................................................................................
100
6
APPENDIX....................................................................................................................................
103
REFERENCES.........................................................................................................................................
113
|
| any_adam_object | 1 |
| author | Kirchweger, Peter |
| author_GND | (DE-588)1175224162 |
| author_facet | Kirchweger, Peter |
| author_role | aut |
| author_sort | Kirchweger, Peter |
| author_variant | p k pk |
| building | Verbundindex |
| bvnumber | BV045447866 |
| ctrlnum | (OCoLC)1136268323 (DE-599)DNB1172683050 |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV045447866 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:18:21Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030833303 |
| oclc_num | 1136268323 |
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| owner_facet | DE-29T DE-29 DE-355 DE-BY-UBR |
| physical | xi, 126 Seiten Illustrationen, Diagramme 21 cm |
| publishDate | 2018 |
| publishDateSearch | 2018 |
| publishDateSort | 2018 |
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| spelling | Kirchweger, Peter Verfasser (DE-588)1175224162 aut Structural characterization of a type 1 secretion systems from Salmonella = Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella vorgelegt von Peter Kirchweger Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella Erlangen ; Nürnberg [2018] xi, 126 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Friedrich-Alexander Universität Erlangen-Nürnberg 2018 Strukturaufklärung (DE-588)4183788-5 gnd rswk-swf Sekretion (DE-588)4124179-4 gnd rswk-swf Salmonella (DE-588)4178963-5 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Salmonella (DE-588)4178963-5 s Sekretion (DE-588)4124179-4 s Proteine (DE-588)4076388-2 s Strukturaufklärung (DE-588)4183788-5 s DE-604 B:DE-101 application/pdf http://d-nb.info/1172683050/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030833303&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kirchweger, Peter Structural characterization of a type 1 secretion systems from Salmonella = Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella Strukturaufklärung (DE-588)4183788-5 gnd Sekretion (DE-588)4124179-4 gnd Salmonella (DE-588)4178963-5 gnd Proteine (DE-588)4076388-2 gnd |
| subject_GND | (DE-588)4183788-5 (DE-588)4124179-4 (DE-588)4178963-5 (DE-588)4076388-2 (DE-588)4113937-9 |
| title | Structural characterization of a type 1 secretion systems from Salmonella = Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella |
| title_alt | Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella |
| title_auth | Structural characterization of a type 1 secretion systems from Salmonella = Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella |
| title_exact_search | Structural characterization of a type 1 secretion systems from Salmonella = Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella |
| title_full | Structural characterization of a type 1 secretion systems from Salmonella = Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella vorgelegt von Peter Kirchweger |
| title_fullStr | Structural characterization of a type 1 secretion systems from Salmonella = Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella vorgelegt von Peter Kirchweger |
| title_full_unstemmed | Structural characterization of a type 1 secretion systems from Salmonella = Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella vorgelegt von Peter Kirchweger |
| title_short | Structural characterization of a type 1 secretion systems from Salmonella |
| title_sort | structural characterization of a type 1 secretion systems from salmonella strukturelle charakterisierung eines typ 1 sekretionssystem aus salmonella |
| title_sub | = Strukturelle Charakterisierung eines Typ 1 Sekretionssystem aus Salmonella |
| topic | Strukturaufklärung (DE-588)4183788-5 gnd Sekretion (DE-588)4124179-4 gnd Salmonella (DE-588)4178963-5 gnd Proteine (DE-588)4076388-2 gnd |
| topic_facet | Strukturaufklärung Sekretion Salmonella Proteine Hochschulschrift |
| url | http://d-nb.info/1172683050/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030833303&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kirchwegerpeter structuralcharacterizationofatype1secretionsystemsfromsalmonellastrukturellecharakterisierungeinestyp1sekretionssystemaussalmonella AT kirchwegerpeter strukturellecharakterisierungeinestyp1sekretionssystemaussalmonella |
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