Redox reactions of proteins at functionalized electrode:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
1998
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 114, III S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV012433376 | ||
| 003 | DE-604 | ||
| 007 | t | ||
| 008 | 990223s1998 gw ad|| m||| 00||| ger d | ||
| 016 | 7 | |a 955723019 |2 DE-101 | |
| 035 | |a (OCoLC)246578557 | ||
| 035 | |a (DE-599)BVBBV012433376 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a ger | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-355 |a DE-11 |a DE-188 | ||
| 100 | 1 | |a Jin, Wen |d 1967- |e Verfasser |0 (DE-588)120811847 |4 aut | |
| 245 | 1 | 0 | |a Redox reactions of proteins at functionalized electrode |c von Wen Jin |
| 264 | 1 | |c 1998 | |
| 300 | |a 114, III S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Potsdam, Univ., Diss. | ||
| 650 | 0 | 7 | |a Proteine |0 (DE-588)4076388-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Redoxreaktion |0 (DE-588)4177303-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Elektronentransfer |0 (DE-588)4151907-3 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Elektronentransfer |0 (DE-588)4151907-3 |D s |
| 689 | 0 | 1 | |a Proteine |0 (DE-588)4076388-2 |D s |
| 689 | 0 | 2 | |a Redoxreaktion |0 (DE-588)4177303-2 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008436606&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-008436606 | |
Datensatz im Suchindex
| _version_ | 1812452891865120768 |
|---|---|
| adam_text |
CONTENTS
PREFACE
ABBREVIATIONS
1. LITERATURE SURVEY (1)
1.1 DIRECT ELECTRON TRANSFER OF PROTEINS AND ENZYMES (1)
1.1.1 DIRECT ELECTRON TRANSFER OF PROTEINS AND ENZYMES- PROBLEMS LIE
AHEAD (1)
1.1.2 METHODS TO ACHIEVE DIRECT PROTEIN AND ENZYME ELECTROCHEMISTRY (2)
1.1.3 CYTOCHROME C (4)
1.1.4 IRON-SULFUR PROTEIN (6)
1.1.5 OTHER PROTEINS (8)
1.1.6 ELECTROCHEMISTRY OF REDOX ENZYMES (8)
1.2 ELECTROCHEMICAL METHOD FOR THE INVESTIGATION OF PROTEIN-ENZYME
INTERACTION (10)
1.2.1 CYCLIC VOLTAMMOGRAM FOR A REVERSIBLE CHARGE TRANSFER PROCESS (11)
1.2.2 REVERSIBLE CHARGE TRANSFER FOLLOWED BY CATALYTIC REACTION (12)
1.3 FUNCTIONALIZED ELECTRODE SURFACE FOR ENZYME BINDING (14)
1.4 SCOPE OF THIS WORK (16)
2. EXPERIMENTAL
(17)
2.1 MATERIALS AND REAGENTS (17)
2.2 APPARATUS AND INSTRUMENTS (18)
2.3 METHODS (18)
2.3.1 AU ELECTRODE CLEANING (18)
2.3.2 ELECTRODE MODIFICATION (20)
2.3.3 CYT C ADSORPTION OR COVALENT BINDING (20)
2.3.4 ELECTROCHEMICAL REDUCTION OF CYT C (20)
2.3.5 SPECTROPHOTOMETRIC REACTION KINETIC MEASUREMENT BETWEEN CYT C
AND ENZYMES (21)
2.3.6 PQQ TITRATION (21)
2.3.7 CONSTRUCTION OF THE MULTI-LAYER SGDH BIOSENSOR (22)
2.3.8 COVALENT IMMOBILIZATION OF PQQ (22)
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/955723019
3.
DIRECT ELECTRON TRANSFER OF PROTEINS AT MODIFIED AU ELECTRODE
(23)
3.1
DIRECT ELECTRON TRANSFER OF CYT C AT MODIFIED AU ELECTRODE (23) ~
3.1.1 ELECTROCHEMISTRY OF CYT C WHILE CYT C IS IN SOLUTION (23)
3.1.2 ELECTROCHEMISTRY OF CYT C WHILE CYT C IS ADSORBED AT THE ELECTRODE
SURFACE (23)
3.1.3 ELECTROCHEMISTRY WHILE CYT C IS COVALENTLY BOUND AT THE ELECTRODE
SURFACE (26)
3.1.4 DISCUSSION (29)
3.2 DIRECT ELECTRON TRANSFER OF [2FE-2S] PROTEIN-ADRENODOXIN AND ITS
MUTANTS AT
MODIFIED AU ELECTRODE (31)
3.2.1 ELECTROCHEMISTRY OF WT-ADX AND ITS MUTANTS AT AU/MUA/PLL ELECTRODE
(33)
3.2.2 ELECTROCHEMISTRY OF WT-ADX AND ITS MUTANTS AT AU/MUA/PAA ELECTRODE
(33)
3.2.3 DISCUSSION (36)
4. ELECTRON TRANSFER REACTION OF CYT C WITH ENZYMES (39)
4.1 ELECTRON TRANSFER REACTION OF CYT C WITH COPPER ENZYMES (39)
4.1.1 ELECTRON TRANSFER REACTION OF CYT C WITH LACCASE (39)
4.1.2 ELECTRON TRANSFER RAECTION OF CYT C WITH SOME OTHER COPPER ENZYMES
(43)
4.1.3 DISCUSSION (43)
4.2
ELECTRON TRANSFER REACTION OF CYT C WITH NADPH-CYTOCHROME P450 REDUCTASE
(46)
4.2.1 HOMOGENEOUS REACTION- CYTOCHROME C IN SOLUTION (46)
4.2.2 HETEROGENEOUS REACTION-CYTOCHROME C AT ELECTRODE SURFACE (49)
4.2.3 DISCUSSION (49)
4.3 ELECTRON TRANSFER REACTION OF CYT C WITH QUINOPROTEIN-GLUCOSE
DEHYDROGENASE (52)
4.3.1 SPECTROPHOTOMETRIC INVESTIGATION (53)
4.3.2 ELECTROCHEMICAL INVESTIGATION (58)
YY
ELECTROCHEMISTRY OF PQQ ON MODIFIED GOLD ELECTRODE (58)
YY
PQQ TITRATION
(58)
YY
MEDIATING EFFECT OF PQQ IN THE ELECTRON TRANSFER REACTION BETWEEN CYT C
AND SGDH
(61)
4.3.3 DISCUSSION (63)
5
. COVALENT IMMOBILIZATION OF SOLUBLE QUINOPROTEIN - GLUCOSE
DEHYDROGENASE AND ITS COFACTOR PQQ (65)
5.1 CONSTRUCTION AND CHARATERIZATION OF MULTILAYER-SGDH ELECTRODE (65)
5.1.1 ELECTRODE CONSTRUCTION (65)
5.1.2 CHARACTERISATION OF THE MULTILAYER-SGDH ELECTRODE (67)
YY
CYCLIC VOLTAMMOGRAMS
(69)
YY
EFFECT OF POTENTIAL
(69)
YY
EFFECT OF GLUCOSE
(69)
YY
EFFECT OF PH AND IONIC STRENGTH
(71)
YY
RESPONSE CHARACTERISTICS ON P-AMINOPHENOL ADDITION
(71)
YY
ELECTRODE STABILITY
(71)
5.2 IMMOBILIZATION OF PQQ (74)
6.
APPLICATION OF CYT C IN THE DETECTION OF 02' AND NO
(77)
6.1 OJ* DETECTION
(77)
YY
USING HYPOXANTHINE AND XOD FOR THE GENERATION OF OI
(77)
YY
USING NADH AND LADHFOR THE GERNERATION OF OJ
(81)
6.2 NO' DETECTION (82)
7. SURFACE ANALYSIS (87)
7.1 SURFACE PLASMA RESONANCE (SPR) (87)
7.2 SCAN TUNNELING MICROSCOPY (STM) (90)
7.3 ATOMIC FORCE MICROSCOPY (AFM) (93)
7.4 ELECTROCHEMICAL METHOD (95)
8. SUMMARY (98)
REFERENCES (103)
PUBLICATIONS
CURRICULUM VITA |
| any_adam_object | 1 |
| author | Jin, Wen 1967- |
| author_GND | (DE-588)120811847 |
| author_facet | Jin, Wen 1967- |
| author_role | aut |
| author_sort | Jin, Wen 1967- |
| author_variant | w j wj |
| building | Verbundindex |
| bvnumber | BV012433376 |
| ctrlnum | (OCoLC)246578557 (DE-599)BVBBV012433376 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV012433376</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">990223s1998 gw ad|| m||| 00||| ger d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">955723019</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)246578557</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV012433376</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">ger</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-188</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Jin, Wen</subfield><subfield code="d">1967-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)120811847</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Redox reactions of proteins at functionalized electrode</subfield><subfield code="c">von Wen Jin</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1998</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">114, III S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Potsdam, Univ., Diss.</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Proteine</subfield><subfield code="0">(DE-588)4076388-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Redoxreaktion</subfield><subfield code="0">(DE-588)4177303-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Elektronentransfer</subfield><subfield code="0">(DE-588)4151907-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Elektronentransfer</subfield><subfield code="0">(DE-588)4151907-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Proteine</subfield><subfield code="0">(DE-588)4076388-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Redoxreaktion</subfield><subfield code="0">(DE-588)4177303-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008436606&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-008436606</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV012433376 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T16:02:49Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008436606 |
| oclc_num | 246578557 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-11 DE-188 |
| owner_facet | DE-355 DE-BY-UBR DE-11 DE-188 |
| physical | 114, III S. Ill., graph. Darst. |
| publishDate | 1998 |
| publishDateSearch | 1998 |
| publishDateSort | 1998 |
| record_format | marc |
| spelling | Jin, Wen 1967- Verfasser (DE-588)120811847 aut Redox reactions of proteins at functionalized electrode von Wen Jin 1998 114, III S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Potsdam, Univ., Diss. Proteine (DE-588)4076388-2 gnd rswk-swf Redoxreaktion (DE-588)4177303-2 gnd rswk-swf Elektronentransfer (DE-588)4151907-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Elektronentransfer (DE-588)4151907-3 s Proteine (DE-588)4076388-2 s Redoxreaktion (DE-588)4177303-2 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008436606&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Jin, Wen 1967- Redox reactions of proteins at functionalized electrode Proteine (DE-588)4076388-2 gnd Redoxreaktion (DE-588)4177303-2 gnd Elektronentransfer (DE-588)4151907-3 gnd |
| subject_GND | (DE-588)4076388-2 (DE-588)4177303-2 (DE-588)4151907-3 (DE-588)4113937-9 |
| title | Redox reactions of proteins at functionalized electrode |
| title_auth | Redox reactions of proteins at functionalized electrode |
| title_exact_search | Redox reactions of proteins at functionalized electrode |
| title_full | Redox reactions of proteins at functionalized electrode von Wen Jin |
| title_fullStr | Redox reactions of proteins at functionalized electrode von Wen Jin |
| title_full_unstemmed | Redox reactions of proteins at functionalized electrode von Wen Jin |
| title_short | Redox reactions of proteins at functionalized electrode |
| title_sort | redox reactions of proteins at functionalized electrode |
| topic | Proteine (DE-588)4076388-2 gnd Redoxreaktion (DE-588)4177303-2 gnd Elektronentransfer (DE-588)4151907-3 gnd |
| topic_facet | Proteine Redoxreaktion Elektronentransfer Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008436606&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT jinwen redoxreactionsofproteinsatfunctionalizedelectrode |