The hepatic response following infection with Listeria monocytogenes:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Giessen
VVB Laufersweiler
2013
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Edition scientifique
|
| Schlagworte: | |
| Online-Zugang: | http://d-nb.info/1034043544/04 Inhaltsverzeichnis |
| Beschreibung: | Nebent.: Hepatic response to L. monocytogenes |
| Beschreibung: | 218 S. Ill., graph. Darst. |
| ISBN: | 9783835960077 |
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TABLE OF CONTENTS
TABLE OF CONTENTS
TABLE O F CONTENTS - - - 4
ABBREVIATIONS 7
1. INTRODUCTION 10
1.1 GENERAL MICROBIOLOGY O F LISTERIA SSP 10
1.2 VIRULENCE FACTORS 11
1.2.1 LISTERIOLYSIN (LLO) 11
1.2.1.1 CHARACTERIZATION O F LLO AND ITS ROLE IN ESCAPE FROM THE
PHAGOSOME 11
1.2.1.2 OTHER ROLES O F LLO IN INFECTION 12
1.2.2 PHOSPHOLIPASES 13
1.2.3 ACTA AND ACTIN-BASED MOTILITY 13
1.2.4 INTENIALINS 14
1.2.5 P60 (MUREM HYDROLASE) 15
1.2.6 CLPC 15
1.2.7 REGULATION O F THE VIRULENCE GENE CLUSTER 16
1.3 INTRACELLULAR INFECTIOUS CYCLE O F L. MONOCYTOGENES 16
1.3.1 INTERNALIZATION 17
1.3.2 INTRACELLULAR PROLIFERATION AND INTERCELLULAR SPREAD 17
1.4 HOST RESPONSE UPON INFECTION WITH L. MONOCYTOGENES 19
1.4.1 COURSE O F INFECTION IN LIVER AND SPLEEN 19
1.4.2 INNATE IMMUNE RESPONSE 20
1.4.2.1 NEUTROPHILS 20
1.4.2.2 MACROPHAGES 21
1.4.2.3 KUPFFER CELLS 22
1.4.2.4 PATHOGEN RECOGNITION RECEPTORS 22
1.4.2.5 INFLAMMATORY CYTOKINES 23
1.4.2.6 AUTOPHAGY 24
1.4.3 ADAPTIVE IMMUNE RESPONSE 25
1.4.3.1 ANTIGEN PRESENTATION 25
1.4.3.2 T CELL RESPONSE AND PROTECTIVE IMMUNITY 26
1.5 PATHOGENESIS O F INFECTION WITH L. MONOCYTOGENES.... 26
1.5.1 ENTTY AND COLONIZATION O F HOST TISSUES CROSSING THE INTESTINAL
BARRIER 26
1.5.2 INFECTION O F THE LIVER 27
1.5.3 INFECTION O F THE SPLEEN 28
1.5.4 INFECTION O F THE GRAVID UTERUS, FETUS AND THE CNS 29
1.5.5 PATHOGENICITY OF LISTERIA INFECTION 30
1.6 EPIDEMIOLOGICAL ASPECTS O F LISTERIOSIS 31
1.7 CLINICAL PHENOTYPE O F LISTERIOSIS 32
1.8 LISTERIA AS A MODEL 32
1.9 MICROARRAY TECHNOLOGY 33
1.10 AIMS OF THIS WORK 34
2. MATERIAL AND METHODS 36
2.1 STUDY DESIGN 36
2.1.1 ANIMALS 37
2.1.2 BACTERIAL CULTURES AND INFECTION ASSAY 37
2.1.3 ISOLATION O F TOTAL RNA FROM ORGANS 37
2.1.4 CODELINK WHOLE GENOME MOUSE CHIP DETAILS 39
2.1.5 ARRAY DESIGN AND FABRICATION 40
2.1.6 MICROARRAY HYBRIDIZATION AND SCANNING 40
2.1.7 SPOT QUANTIFICATION AND CODELINK IMAGENE BATCH AUTOMATION SOFTWARE
41
4
HTTP://D-NB.INFO/1034043544
IMAGE 2
TABLE OF CONTENTS
2.1.8 DESCRIPTION O F THE CODELINK DATA FILE 42
2.1.8.1 THRESHOLD AND QUALITY FLAG 43
2.1.9 QUALITY CONTROL O F THE RAW DATA 43
2.1.9.1 CLEANTABLE MODULE 44
2.1.9.2 THRESHOLD MODULE 45
2.1.9.3 IMPUTATION O F EMPTY FIELDS 45
2.1.9.4 OUTLIER FILTER MODULE 45
2.1.9.5 QUANTILE NORMALIZATION AND LOGARITHM 46
2.1.9.6 CORRELATION MATRIX AND MICROARRAY OUTLIER FILTER (MOF) 46
2.1.9.7 MEAN VERSUS AVERAGE PLOT (MVA) 47
2.1.9.8 QUALITY CONTROL ALGORITHM 47
2.1.10 ANALYSIS METHODOLOGY 49
2.1.10.1 IDENTIFICATION O F DIFFERENTIALLY EXPRESSED GENES USING THE
RANK PRODUCTS (RP) TOOL 49
2.1.10.2 ANNOTATION O F SIGNIFICANTLY DEREGULATED GENES 51
2.1.10.3 ENRICHMENT O F OVERREPRESENTED CATEGORIES 51
2.1.10.4 LITERATURE SEARCH 52
2.1.10.5 CLUSTER ANALYSIS WITH DCHIP AND BIOLAYOUT 53
2.1.10.6 PATHWAY AND NETWORK ANALYSIS BY INGENUITY PATHWAY ANALYSIS
(IPA) 54
2.1.11 CDNA SYNTHESIS FOR QUANTITATIVE REAL-TIME PLOYMERASE CHAIN
REACTION 54
2.1.12 QUANTITATIVE REAL-TIME PCR AND DATA ANALYSIS 55
2.1.13 ELECTROPHORESIS O F PRIMER PRODUCTS 56
2.1.14 PREPARATION O F CIYOSECTIONS 57
2.1.15 LIPID DROPLETS STAINING WITH OIL RED O 57
2.1.16 CELL CULTURES, LIPID DROPLETS WITH BODIPY 493/503 AND
IMMUNOFLUORESENCE STAINING 57
2.1.17 TRIASCIN C TREATMENT 59
2.1.18 MEASUREMENT O F LIVER AND LIPID SERUM PARAMETERS 59
2.1.19 WORKFLOW FOR ANALYSIS O F DATA ACQUIRED BY MICROARRAYS 59
3. RESULTS 61
3.1 QUALITY CONTROL O F MICROARRAYS 61
3.1.1 CORRELATION MATRIX AND MVA PLOTS 62
3.1.2 RANKPRODUCTS 64
3.2 HEPATIC RESPONSE UPON INFECTION WITH L. MONOCYTOGENES 65
3.2.1 GLOBAL VIEW O F HEPATIC RESPONSE TOL. MONOCYTOGENES 65
3.2.2 INNATE AND ADAPTIVE IMMUNE RESPONSE INTERSECT IN A GLOBAL VIEW
UPON CHALLENGE WITH L. MONOCYTOGENES 81
3.2.3 HEPATIC LIPID METABOLIC AND IMMUNE RESPONSE ARE RECIPROCALLY
REGULATED IN A GLOBAL VIEW 83
3.2.4 TRANSIENT GENE DEREGULATION IS ASSOCIATED WITH THE ACCUMULATION O
F INTRACELLULAR LIPID DROPLETS 84
3.2.5 L MONOCYTOGENES INDUCES THE FORMATION O F LIPID DROPLETS THROUGH
AN ALTERNATIVE PATHWAY 87
3.2.5.1 L. MONOCYTOGENES INDUCES LXR DEPENDENT GENE EXPRESSION MEDIATED
BY INCREASING LEVELS O F OXYSTEROLS 91
3.2.5.2 LXR RESPONSIVE GENES INVOLVED IN IMMUNE RESPONSE OR APOPTOSIS
ARE DISTINCTLY REGULATED FROM THE GENES OF METABOLISM 92
3.2.5.3 ROLE O F LXR - REGULATED GENES 93
3.2.5.4 POSTTRANSLATIONAL MODIFICATION O F LXR POTENTIALLY CONTROLS THE
GENE EXPRESSION PANEL O F LXR RESPONSIVE GENES 94
3.2.5.5 LXR-A ACCUMULATES IN THE NUCLEUS IN AN IN VITRO INFECTION MODEL
WITH L. MONOCYTOGENES 94
3.2.5.6 DYNAMIC DEREALIZATION O F LXR THAT IS PHOSPHORYLATED AT S198 96
3.2.6 INFECTION WITH L. MONOCYTOGENES LEADS TO ALTERED SERUM LIPID
PROFILE AND TRANSIENTLY IMPAIRED LIVER FUNCTION 98
3.2.7 INNATE IMMUNE RESPONSE BY INTERACTING KUPFFER CELLS AND
NEUTROPHILS BY CALGRANULINS 100
3.2.8 L MONOCYTOGENES MODULATES THE EARLY INNATE RESPONSE BY INHIBITION
O F ANTIMICROBIAL PEPTIDES 101
3.2.9 INTERSECTION O F CLASSICAL AND NON-CLASSICAL MHC-IB ANTIGEN
PRESENTATION PATHWAYS FOLLOWING LISTERIAL INFECTION 102
3.2.9.1 INDUCTION O F H2-M3 INDEPENDENT MHC-IB ANTIGEN PRESENTATION 103
3.2.9.2 INDUCTION O F THE IMMUNOPROTEASOME AND POTENTIAL ROLE FOR P60 IN
THE ACTIVATION O F NK CELLS 105
3.2.10 VALIDATION O F MICROARRAYS BY QRT-PCR. 108
4. DISCUSSION - . . . . 1 1 2
5. REFERENCES - - 1 2 5
6. FIGURE AND TABLE LEGENDS 147
5
IMAGE 3
TABLE OF CONTENTS
7. SUMMARY ... . . . 156
8. ZUSAMMENFASSUNC 159
9. LIST O F OWN PUBLICATIONS AND POSTERS 162
10. ERKLARUNG 166
11. ACKNOWLEDGEMENTS 167
12. APPENDIX 168
6
|
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| discipline | Medizin |
| edition | 1. Aufl. |
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| indexdate | 2024-07-10T00:58:23Z |
| institution | BVB |
| isbn | 9783835960077 |
| language | English |
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| spelling | Izar, Benjamin Verfasser aut The hepatic response following infection with Listeria monocytogenes vorgelegt von Benjamin Izar Hepatic response to L. monocytogenes 1. Aufl. Giessen VVB Laufersweiler 2013 218 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Edition scientifique Nebent.: Hepatic response to L. monocytogenes Zugl.: Giessen, Univ., Diss., 2013 Immunreaktion (DE-588)4133841-8 gnd rswk-swf Leberkrankheit (DE-588)4034937-8 gnd rswk-swf Listeria monocytogenes (DE-588)4203141-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Leberkrankheit (DE-588)4034937-8 s Listeria monocytogenes (DE-588)4203141-2 s Immunreaktion (DE-588)4133841-8 s DE-604 B:DE-101 application/pdf http://d-nb.info/1034043544/04 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026998130&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Izar, Benjamin The hepatic response following infection with Listeria monocytogenes Immunreaktion (DE-588)4133841-8 gnd Leberkrankheit (DE-588)4034937-8 gnd Listeria monocytogenes (DE-588)4203141-2 gnd |
| subject_GND | (DE-588)4133841-8 (DE-588)4034937-8 (DE-588)4203141-2 (DE-588)4113937-9 |
| title | The hepatic response following infection with Listeria monocytogenes |
| title_alt | Hepatic response to L. monocytogenes |
| title_auth | The hepatic response following infection with Listeria monocytogenes |
| title_exact_search | The hepatic response following infection with Listeria monocytogenes |
| title_full | The hepatic response following infection with Listeria monocytogenes vorgelegt von Benjamin Izar |
| title_fullStr | The hepatic response following infection with Listeria monocytogenes vorgelegt von Benjamin Izar |
| title_full_unstemmed | The hepatic response following infection with Listeria monocytogenes vorgelegt von Benjamin Izar |
| title_short | The hepatic response following infection with Listeria monocytogenes |
| title_sort | the hepatic response following infection with listeria monocytogenes |
| topic | Immunreaktion (DE-588)4133841-8 gnd Leberkrankheit (DE-588)4034937-8 gnd Listeria monocytogenes (DE-588)4203141-2 gnd |
| topic_facet | Immunreaktion Leberkrankheit Listeria monocytogenes Hochschulschrift |
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