Development and application of a chemogenetic calcium recorder:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Heidelberg
[2023]
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | vii, 162 Seiten Illustrationen, Diagramme 30 cm |
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MARC
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| 100 | 1 | |a Huppertz, Magnus-Carsten |d 1991- |e Verfasser |0 (DE-588)1319183794 |4 aut | |
| 245 | 1 | 0 | |a Development and application of a chemogenetic calcium recorder |c presented by M. Sc. Magnus-Carsten Huppertz |
| 264 | 1 | |a Heidelberg |c [2023] | |
| 300 | |a vii, 162 Seiten |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Ruprecht-Karls-University Heidelberg |d 2023 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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Datensatz im Suchindex
| _version_ | 1816443028432224256 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
ABSTRACT
.
I
ZUSAMMENFASSUNG
.
II
CHAPTER
1
-
INTRODUCTION
.
1
1.1
VISUALIZING
THE
BRAIN
IN
ACTION
.
1
1.1.1
NEURONS
.
1
1.1.2
NEURAL
ACTIVITY
AND
THE
IMPORTANCE
OF
INTRACELLULAR
CA
2+
SIGNALING
.
2
1.1.3
GLIA
CELLS
.
4
1.1.4
GLIOBLASTOMA
-
MALIGNANT
CELL
NETWORKS
IN
THE
BRAIN
.
4
1.2
RECORDING
INFORMATION
FROM
THE
BRAIN
IS
CHALLENGING
.
6
1.2.1
THE
APPEALS
OF
FLUORESCENCE
IMAGING
FOR
BIOLOGICAL
SPECIMEN
.
8
1.2.2
PRACTICAL
TRADE-OFFS
FOR
FLUORESCENCE
IMAGING
OF
BIOLOGICAL
TISSUES
.
9
1.3
FLUORESCENCE
TAGGING
STRATEGIES
IN
VIVO
.
10
1.3.1
FLUORESCENT
PROTEINS
.
11
1.3.2
SYNTHETIC
FLUOROPHORES
AND
LIGANDS
.
12
1.3.3
METHODS
TO
COVALENTLY
LABEL
GENETICALLY-ENCODED
TAGS
WITH
FLUOROPHORES
.
13
1.4
RECORDING
NEURAL
ACTIVITY
-
REAL
TIME
VS.
POST
HOC
.
15
1.4.1
REAL-TIME
MONITORING
OF
NEURONAL
ACTIVITY
THROUGH
INTRACELLULAR
CA
2+
SIGNALING
.
16
1.4.2
LARGE-SCALE
FUNCTIONAL
MAPPING
OF
NEURONAL
ACTIVITY
USING
CA
2+
RECORDERS
.
18
CHAPTER
2
-
OBJECTIVES
.
24
CHAPTER
3
-
RESULTS
.25
3.1
DEVELOPMENT
OF
A
CHEMOGENETIC
RECORDERS
FOR
CA
2+
.
25
3.1.1
RATIONAL
.25
3.1.2
CA
2+
DEPENDENT
PROTEIN
LABELING
USING
A
SPLIT-HALOTAG
SYSTEM
.25
3.2
CHARACTERIZATION
OFCAPROLA
IN
MAMMALIAN
CELLS
.
28
3.2.1
CHARACTERIZATION
OF
EARLY
CAPROLA
VARIANTS
FEATURING
A
9-MER
H-PEPTIDE
.
29
3.2.2
CHARACTERIZATION
OF
CAPROLA
WITH
A
10-MER
H-PEPTIDE
IN
CELL
LINES
AND
NEURONS
.
29
3.2.3
EXAMINATION
OF
THE
CA
2+
RESPONSIVENESS
AND
FLUORESCENT
LIGAND
SCOPE
OF
CAPROLA
.
32
3.2.4
RESISTANCE
OF
CAPROLA
SIGNAL
TO
CHEMICAL
FIXATION
PROTOCOLS
.
33
3.2.5
EVALUATION
OF
THE
TEMPORAL
RESOLUTION
OF
CAPROLA
.36
3.2.6
ASSESSING
THE
CA
2+
SENSITIVITY
OF
CAPROLA
USING
A
FOCUSED
DRUG
SCREEN
.37
TABLE
OF
CONTENTS
3.2.7
RECORDING
SUCCESSIVE
CA
2+
EVENTS
IN
MULTI-COLOR
.
38
3.3
CHARACTERIZATION
OF
CAPROLA
IN
CULTURED
PRIMARY
NEURONS
.
42
3.3.1
ASSESSING
THE
CA
2+
SENSITIVITY
OF
CAPROLA
IN
CULTURED
PRIMARY
NEURONS
.
43
3.3.2
CAPROLA
RECORDINGS
FROM
CYTOSOLIC
[CA
2+
]
EVOKED
BY
ELECTRICAL
FIELD
STIMULATION
.
46
3.3.3
APPLICATION
OF
CAPROLA
TO
RECORD
CA
2+
FROM
CELLULAR
ORGANELLES
.
47
3.4
APPLICATION
OF
CAPROLA
TO
DECIPHER
THE
GENETIC
HETEROGENEITY
OF
GLIOBLASTOMA
NETWORKS
.
50
3.4.1
FUNCTIONALITY
VALIDATION
OF
CAPROLA
IN
NETWORK-FORMING
GLIOBLASTOMA
CULTURES
.
50
3.4.2
DETERMINATION
OF
CAPROLA
LABELING
CONDITIONS
IN
GLIOBLASTOMA
NETWORKS
.
53
3.4.3
MANIPULATION
OF
INTRACELLULAR
CA
2+
LEVELS
IN
GLIOBLASTOMA
NETWORKS
.
55
3.4.4
RNA
FINGERPRINT
ANALYSIS
OF
GLIOBLASTOMA
CELLS
.
55
3.5
NEURONAL
ACTIVITY
RECORDINGS
FROM
BEHAVING
LARVAL
ZEBRAFISH
.58
3.5.1
CHARACTERIZATION
OF
FLUOROPHORES
TO
LABEL
HALOTAG7
INSIDE
THE
LARVAL
ZEBRAFISH
BRAIN
.
58
3.5.2
PROFILING
OF
FLUORESCENT
LIGAND
UPTAKE
AND
DISTRIBUTION
WITHIN
LARVAL
ZEBRAFISH
BRAINS
.
61
3.5.3
CAPROLA
LABELING
CONDITION
OPTIMIZATION
IN
ZEBRAFISH
.64
3.5.4
RECORDING
NEURONAL
CA
2+
IN
VISUALLY
STIMULATED
ZEBRAFISH
.
65
3.5.5
.
CA
2+
RECORDINGS
FROM
FREELY
BEHAVING
ZEBRAFISH
.68
CHAPTER
4
-
DISCUSSION
.
70
4.1
CAPROLA
PERFORMS
ROBUSTLY
IN
MAMMALIAN
CELLS
.
70
4.2
SEQUENTIAL
CA
2+
RECORDINGS
IN
MULTI-COLOR
.
73
4.3
CA
2+
RECORDINGS
CAN
BE
OBTAINED
IN
CULTURED
PRIMARY
NEURONS
BEYOND
THE
CYTOSOL
.
74
4.4
CAPROLA
RECORDINGS
IDENTIFIED
GENETIC
PROFILES
IN
GLIOBLASTOMA
OF
MEDICAL
RELEVANCE
.
76
4.5
CAPROLA
SPECIFICALLY
LABELS
IN
RESPONSE
TO
NEURONAL
[CA
2+
]
IN
BEHAVING
ZEBRAFISH
LARVAE
.
77
4.6
COMPARISONS
OF
CAPROLA
TO
AVAILABLE
CA
2
*
RECORDER
SYSTEMS
.
81
CHAPTER
5
-
CONCLUSIONS
AND
OUTLOOK
.
85
CHAPTER
6
-
METHODS
.
87
APPENDIX
.99
BIBLIOGRAPHY
.
145
ACKNOWLEDGMENTS
.
159 |
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| author | Huppertz, Magnus-Carsten 1991- |
| author_GND | (DE-588)1319183794 |
| author_facet | Huppertz, Magnus-Carsten 1991- |
| author_role | aut |
| author_sort | Huppertz, Magnus-Carsten 1991- |
| author_variant | m c h mch |
| building | Verbundindex |
| bvnumber | BV050040402 |
| ctrlnum | (DE-599)DNB1318569214 |
| discipline | Medizin |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV050040402 |
| illustrated | Illustrated |
| indexdate | 2024-11-22T17:04:23Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-035378163 |
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| owner_facet | DE-355 DE-BY-UBR |
| physical | vii, 162 Seiten Illustrationen, Diagramme 30 cm |
| publishDate | 2023 |
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| spelling | Huppertz, Magnus-Carsten 1991- Verfasser (DE-588)1319183794 aut Development and application of a chemogenetic calcium recorder presented by M. Sc. Magnus-Carsten Huppertz Heidelberg [2023] vii, 162 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Ruprecht-Karls-University Heidelberg 2023 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1318569214/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=035378163&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p dnb 20240220 DE-101 https://d-nb.info/provenance/plan#dnb 2\p dnb 20240220 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Huppertz, Magnus-Carsten 1991- Development and application of a chemogenetic calcium recorder |
| subject_GND | (DE-588)4113937-9 |
| title | Development and application of a chemogenetic calcium recorder |
| title_auth | Development and application of a chemogenetic calcium recorder |
| title_exact_search | Development and application of a chemogenetic calcium recorder |
| title_full | Development and application of a chemogenetic calcium recorder presented by M. Sc. Magnus-Carsten Huppertz |
| title_fullStr | Development and application of a chemogenetic calcium recorder presented by M. Sc. Magnus-Carsten Huppertz |
| title_full_unstemmed | Development and application of a chemogenetic calcium recorder presented by M. Sc. Magnus-Carsten Huppertz |
| title_short | Development and application of a chemogenetic calcium recorder |
| title_sort | development and application of a chemogenetic calcium recorder |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1318569214/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=035378163&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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