Molecular biological, physiological and isotope chemical analyses of BTEX (bio)degradation processes within a contaminated aquifer:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Leipzig
UFZ
2010
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| Series: | PhD Dissertation
2010,8 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | IV, 189 S. Ill., graph. Darst. |
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| adam_text | MOLECULAR BIOLOGICAL, PHYSIOLOGICAL AND ISOTOPE CHEMICAL ANALYSES OF
BTEX (BIO)DEGRADATION PROCESSES WITHIN A CONTAMINATED AQUIFER * BY THE
FACULTY OF CHEMISTRY AND PHYSICS OF THE TECHNISCHE UNIVERSITAT
BERGAKADEMIE FREIBERG APPROVED THESIS TO ATTAIN THE ACADEMIC DEGREE OF
DOCTOR RERUM NATURALIUM DR. RER. NAT. SUBMITTED BY DIPLOMA GEOECOLOGIST
STEFFI HERRMANN BOM ON THE 16.06.1980 IN DRESDEN ASSESSORS: PROF. DR.
MICHAEL SCHLOMANN, FREIBERG PROF. DR. LORENZ ADRIAN, BERLIN DR.
HANS-HERMANN RICHNOW, LEIPZIG DATE OF THE DEFENCE: 28.05.2010 CONTENTS
ABSTRACT . I CONTENTS ILL 1 INTRODUCTION 1 .1 BTEX CONTAMINATION 2 .2
AEROBIC DEGRADATION OF BTEX COMPOUNDS 3 .3 ANAEROBIC DEGRADATION OF
BTEX COMPOUNDS 5 .4 NATURAL ATTENUATION AND ENHANCED NATURAL ATTENUATION
OF BTEX-CONTAMINATED AQUIFERS 10 .5 VERIFICATION OF IN SITU
BIODEGRADATION 13 .6 STABILE ISOTOPE BASED METHODS FOR THE ASSESSMENT OF
MICROBIAL ACTIVITY 19 1.6.1 COMPOUND SPECIFIC ISOTOPE ANALYSIS (CSIA) 20
1.6.2 STABILE ISOTOPE PROBING (SIP) 28 1.7 OUTLINE OF THE THESIS 32
ENRICHMENT OF ANAEROBIC BENZENE-DEGRADING MICROORGANISMS BY IN SITU
MICROCOSMS 33 2.1 INTRODUCTION 34 2.2 MATERIALS AND METHODS . . 35 2.2.1
DESCRIPTION OF THE FIELD SITE 35 2.2.2 PREPARATION OF IN SITU MICROCOSMS
36 2.2.3 CHEMICAL ANALYSES 37 2.2.4 MOLECULAR-GENETIC ANALYSES 38 2.2.5
CONFOCAL LASER SCANNING MICROSCOPY (CLSM) 39 2.2.6 DETERMINATION OF
TOTAL CELL COUNTS 39 2.3 RESULTS AND DISCUSSION 40 2.3.1 ENRICHMENT OF
BENZENE-DEGRADING MICROORGANISMS 40 2.3.2 MICROBIAL COLONIZATION OF SAND
AND LAVA GRANULES DURING IN SITU EXPOSURE 44 2.3.3 PHYLOGENETIC
DIVERSITY OF COLONIZING MICROBIAL COMMUNITIES 46 IDENTIFICATION OF
ANAEROBIC BENZENE-ASSIMILATING ORGANISMS BY DNA STABLE ISOTOPE
PROBING.... 51 3.1 INTRODUCTION 52 3.2 EXPERIMENTAL PROCEDURES 54 3.2.1
CULTIVATION PROCEDURES AND SET UP OF THE STABLE ISOTOPE PROBING
EXPERIMENT 54 3.2.2 DENSITY GRADIENT CENTRIFUGATION OF DNA 55 3:2.3
T-RFLP ANALYSES 56 3.2.4 CLONING AND SEQUENCING OF 16S RRNA GENES 56
3.2.5 CHEMICAL ANALYSES 56 3.3 RESULTS 57 3.3.1 BENZENE DEGRADATION
PATTERN IN MICROCOSMS SPIKED WITH L3 C 6 -BENZENE OR NATURAL BENZENE 57
3.3.2 IDENTIFICATION OF BENZENE-ASSIMILATING ORGANISMS BY STABLE ISOTOPE
PROBING OF DNA (DNA-SIP) . 59 3.4 DISCUSSION 63 3.5 SUPPORTING
INFORMATION 68 COMBINED CARBON AND HYDROGEN ISOTOPE FRACTIONATION
ANALYSIS FOR ELUCIDATING BENZENE BIODEGRADATION PATHWAYS 71 4.1
INTRODUCTION 72 4.2 MATERIALS AND METHODS 74 4.2.1 MICROORGANISMS AND
CULTIVATION CONDITIONS 74 4.2.2 QUANTIFICATION OF ISOTOPE FRACTIONATION
75 4.3 RESULTS AND DISCUSSION 76 4.3.1 CARBON ISOTOPE FRACTIONATION 76
4.3.2 HYDROGEN ISOTOPE FRACTIONATION 78 4.3.3 TWO-DIMENSIONAL ISOTOPE
FRACTIONATION INVESTIGATIONS 81 4.3.4 ENVIRONMENTAL IMPLICATIONS 82 4.4
SUPPORTING INFORMATION 84 IV 4.4.1 DEGRADATION EXPERIMENTS 84 4.4.2
ANALYTICAL METHODS 85 4.4.3 QUANTIFICATION OF ISOTOPE FRACTIONATION 86
4.4.4 TWO-DIMENSIONAL ISOTOPE FRACTIONATION APPROACH 87 5
CHARACTERIZATION OF ANAEROBIC XYLENE BIODEGRADATION BY TWO DIMENSIONAL
ISOTOPE FRACTIONATION ANALYSIS 89 5.1 INTRODUCTION 90 5.2 EXPERIMENTAL
PROCEDURES 92 5.2.1 MICROORGANISMS AND CULTIVATION CONDITIONS 92 5.2.2
ISOTOPE FRACTIONATION EXPERIMENTS 92 5.2.3 ANALYTICAL METHODS 93 5.2.4
STABLE ISOTOPE ANALYSIS 94 5.2.5 QUANTIFICATION OF ISOTOPE FRACTIONATION
94 5.2.6 DNA PREPARATION AND SEQUENCING OF BSSA GENE FRAGMENTS 96 5.3
RESULTS 97 5.3.1 CHARACTERISTICS OF XYLENE-DEGRADING ENRICHMENT CULTURES
97 5.3.2 CHARACTERISTICS OF TOLUENE-, AND XYLENE-DEGRADING PURE CULTURES
97 5.3.3 CARBON AND HYDROGEN ISOTOPE FRACTIONATION OF XYLENE DEGRADATION
98 5.3.4 CARBON AND HYDROGEN ISOTOPE FRACTIONATION OF TOLUENE
DEGRADATION 100 5.3.5 TWO DIMENSIONAL ISOTOPE FRACTIONATION ANALYSES 100
5.4 DISCUSSION 102 5.5 SUPPORTING INFORMATION 105 6 BEOQUE - QUALITATIVE
AND QUANTITATIVE PROOF OF BIODEGRADATION IN A BENZENE-CONTAMINATED
AQUIFER IN THE COURSE OF AN ENHANCED NATURAL ATTENUATION APPROACH 113
6.1 INTRODUCTION 114 6.2 SITE, PROJECT AND METHODS INFORMATION 116 6.2.1
CHARACTERISATION OF THE TEST SITE 116 6.2.2 CHARACTERISATION OF TEST
FIELDS 117 6.2.3 TRACERTEST 119 6.2.4 SAMPLING 120 6.2.5 HYDROCHEMICAL
PARAMETERS 121 6.2.6 ISOTOPE CHEMICAL PARAMETERS . 122 6.2.7
DETERMINATION OF MPN 123 6.2.8 BACTRAP-EXPERIMENTS 123 6.3 RESULTS 127
6.3.1 EXPLORATION OF GROUNDWATER FLOW DIRECTION AND VELOCITY OF TF2 127
6.3.2 HYDROCHEMICAL GROUNDWATER ANALYSES 128 6.3.3 SOIL VAPOUR
INVESTIGATIONS 132 6.3.4 ISOTOPE CHEMICAL PARAMETERS 133 6.3.5 MPN OF
AEROBIC AND AEROBIC BENZENE-METABOLISING MICROORGANISMS 134 6.3.6
BACTRAP-EXPERIMENTS :. 134 6.4 DISCUSSION AND CONCLUSIONS 138 6.4.1
EFFECTS OF AIR-SPARGING ON HYDROCHEMICAL PARAMETERS 139 6.4.2
DETERMINATION OF GROUND WATER FLOW DIRECTION AND VELOCITY 140 6.4.3
EFFICIENCY OF AIR-SPARGING - CALCULATIONS BASED ON BENZENE REMOVAL 141
6.4.4 THE ROLE OF MICROORGANISMS 144 6.5 SUPPORTING INFORMATION 153 7
SUMMARY AND FINAL CONCLUSIONS 157 7.1 ANAEROBIC BENZENE DEGRADATION 158
7.2 ELUCIDATING DEGRADATION PATHWAYS BY MEANS OF COMPOUND SPECIFIC
ISOTOPE ANALYSIS.. 159 7.3 EVALUATION OF AIR-SPARGING AS A STRATEGY TO
ENHANCE NATURAL ATTENUATION 161 REFERENCES 163 APPENDIX 183
|
| any_adam_object | 1 |
| author | Herrmann, Steffi |
| author_facet | Herrmann, Steffi |
| author_role | aut |
| author_sort | Herrmann, Steffi |
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| building | Verbundindex |
| bvnumber | BV036618308 |
| ctrlnum | (OCoLC)663873075 (DE-599)BSZ327114665 |
| dewey-full | 579.176278 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 579 - Microorganisms, fungi & algae |
| dewey-raw | 579.176278 |
| dewey-search | 579.176278 |
| dewey-sort | 3579.176278 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| spelling | Herrmann, Steffi Verfasser aut Molecular biological, physiological and isotope chemical analyses of BTEX (bio)degradation processes within a contaminated aquifer Steffi Herrmann Leipzig UFZ 2010 IV, 189 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier PhD Dissertation 2010,8 Zugl.: Freiberg, Techn. Univ., Diss., 2010 BTX-Aromaten (DE-588)4337078-0 gnd rswk-swf Isotopentrennung (DE-588)4131116-4 gnd rswk-swf Biologischer Abbau (DE-588)4145625-7 gnd rswk-swf Grundwasserverschmutzung (DE-588)4020822-9 gnd rswk-swf Chemische Analyse (DE-588)4009840-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content BTX-Aromaten (DE-588)4337078-0 s Biologischer Abbau (DE-588)4145625-7 s Isotopentrennung (DE-588)4131116-4 s Chemische Analyse (DE-588)4009840-0 s Grundwasserverschmutzung (DE-588)4020822-9 s DE-604 PhD Dissertation 2010,8 (DE-604)BV035421074 2010,8 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020538392&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Herrmann, Steffi Molecular biological, physiological and isotope chemical analyses of BTEX (bio)degradation processes within a contaminated aquifer PhD Dissertation BTX-Aromaten (DE-588)4337078-0 gnd Isotopentrennung (DE-588)4131116-4 gnd Biologischer Abbau (DE-588)4145625-7 gnd Grundwasserverschmutzung (DE-588)4020822-9 gnd Chemische Analyse (DE-588)4009840-0 gnd |
| subject_GND | (DE-588)4337078-0 (DE-588)4131116-4 (DE-588)4145625-7 (DE-588)4020822-9 (DE-588)4009840-0 (DE-588)4113937-9 |
| title | Molecular biological, physiological and isotope chemical analyses of BTEX (bio)degradation processes within a contaminated aquifer |
| title_auth | Molecular biological, physiological and isotope chemical analyses of BTEX (bio)degradation processes within a contaminated aquifer |
| title_exact_search | Molecular biological, physiological and isotope chemical analyses of BTEX (bio)degradation processes within a contaminated aquifer |
| title_full | Molecular biological, physiological and isotope chemical analyses of BTEX (bio)degradation processes within a contaminated aquifer Steffi Herrmann |
| title_fullStr | Molecular biological, physiological and isotope chemical analyses of BTEX (bio)degradation processes within a contaminated aquifer Steffi Herrmann |
| title_full_unstemmed | Molecular biological, physiological and isotope chemical analyses of BTEX (bio)degradation processes within a contaminated aquifer Steffi Herrmann |
| title_short | Molecular biological, physiological and isotope chemical analyses of BTEX (bio)degradation processes within a contaminated aquifer |
| title_sort | molecular biological physiological and isotope chemical analyses of btex bio degradation processes within a contaminated aquifer |
| topic | BTX-Aromaten (DE-588)4337078-0 gnd Isotopentrennung (DE-588)4131116-4 gnd Biologischer Abbau (DE-588)4145625-7 gnd Grundwasserverschmutzung (DE-588)4020822-9 gnd Chemische Analyse (DE-588)4009840-0 gnd |
| topic_facet | BTX-Aromaten Isotopentrennung Biologischer Abbau Grundwasserverschmutzung Chemische Analyse Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020538392&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035421074 |
| work_keys_str_mv | AT herrmannsteffi molecularbiologicalphysiologicalandisotopechemicalanalysesofbtexbiodegradationprocesseswithinacontaminatedaquifer |