Selecting good-quality sperm for use in in-vitro fertilization is a key step in assisted reproduction. For many years purely morphological attributes have been used to assess suitability, but increasingly biochemical and molecular biological techniques are now identifying sperm with the best chances...

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Bibliographic Details

Other Authors:	Agarwal, Ashok (Editor), Henkel, Ralf 1960- (Editor), Majzoub, Ahmad (Editor)
Format:	Electronic eBook
Language:	English
Published:	Cambridge Cambridge University Press 2021
Subjects:	Spermatozoa / Physiology Infertility, Male
Online Access:	BSB01 FHN01 Volltext
Summary:	Selecting good-quality sperm for use in in-vitro fertilization is a key step in assisted reproduction. For many years purely morphological attributes have been used to assess suitability, but increasingly biochemical and molecular biological techniques are now identifying sperm with the best chances of producing viable and healthy embryos. Focusing on modern sperm function testing, this manual provides technical details of commonly used tests and gives an overview of the laboratory techniques used to evaluate sperm samples. Covering a variety of testing methods in detail, from manual and computer-assisted semen analysis to zona pellucida binding assays, and tests assessing sperm DNA damage such as the TUNEL assay. Describing the underlying science, practical advice for performing the tests is given, including tips for optimizing outcomes and trouble-shooting. This is an essential guide for reproductive medicine specialists, clinical andrologists, urologists and gynecologists working with sub-fertile men
Item Description:	Title from publisher's bibliographic system (viewed on 09 Apr 2021) Basic Semen Examination -- Standard semen analysis : Computer-assisted semen analysis -- Standard semen analysis : home sperm testing -- Standard semen analysis : Leukocytospermia -- Standard semen analysis : Sperm Morphology Evaluation -- Sperm vitality : eosinnigrosin dye exclusion -- Sperm vitality : the hypoosmotic swelling test -- Determination of mitochondrial membrane potential by flow cytometry in human sperm cells -- Capacitation and acrosome reaction : fluorescence techniques to determine acrosome reaction -- Capacitation and acrosome reaction : Histochemical techniques to assess the acrosome reaction -- Zona binding : competitive sperm-binding assay -- Zona binding-Hemizona assay -- Oolemma Binding : Sperm Penetration Assay -- Oxidative Stress Testing : Direct Tests -- Oxidative stress in male Infertility : Indirect tests -- Chromatin condensation : Aniline blue staining -- Chromatin condensation : chromomycin A3 (CMA3) stain -- Chromatin Condensation : Toluidine Blue Staining -- TdT-mediated dUTP nickend-labelling (TUNEL) assay -- DNA damage : sperm chromatin structure assay (SCSA) -- Sperm DNA damage measured by the Comet assay -- Sperm Chromatin Dispersion test -- Fluorescent in-situ hybridization -- Clinical Value of Sperm Function Tests -- Sperm Proteomics
Physical Description:	1 Online-Ressource (xv, 270 Seiten)
ISBN:	9781108878715
DOI:	10.1017/9781108878715