Effects of Flt3, Flk2-ligand and Interleukin-7 on in vitro growth of acute lymphoblastic leukemia cells:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
1998
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Frankfurt (Main), Univ., Diss., 2000 |
| Beschreibung: | VII, 112 S. Ill., graph. Darst. : 21 cm |
Internformat
MARC
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| 100 | 1 | |a Hemmati, Philipp Georg |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Effects of Flt3, Flk2-ligand and Interleukin-7 on in vitro growth of acute lymphoblastic leukemia cells |c vorgelegt von Philipp Hemmati |
| 264 | 1 | |c 1998 | |
| 300 | |a VII, 112 S. |b Ill., graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Frankfurt (Main), Univ., Diss., 2000 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009018426&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009018426 | |
Datensatz im Suchindex
| _version_ | 1807773136822730752 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
PAGE
1.
INTRODUCTION
1
1.1
NORMAL
HEMATOPOIESIS
1
1.2
LEUKEMIA
3
1.2.1
DEFINITION
3
1.2.2
CLASSIFICATION
4
1.2.4
EPIDEMIOLOGY
6
1.2.5
ETIOLOGY
7
1.3
OVERVIEW
8
1.4
MECHANISMS
OF
ONCOGENESIS
10
1.4.1
RECEPTOR-MEDIATED
SIGNALING
AND
CELLULAR
ONCOGENES
10
1.4.2
PROGRAMMMED
CELL
DEATH
(APOPTOSIS)
13
1.5
TISSUE
CULTURE
OF
ALL
CELLS
14
1.6
THE
FLT3/FLK-2-RECEPTOR
AND
ITS
LIGAND
17
1.6.1
THE
FLT3/FLK-2-RECEPTOR
18
1.6.1.1
CLONING
OF
THE
FLT3-/FLK-2-RECEPTOR
18
1.6.1.2
STRUCTURE
AND
FUNCTION
OF
THE
FLT3/FLK-2-RECEPTOR
19
1.6.1.3
CYTOPLASMIC
SIGNALING
MEDIATORS
OF
THE
FLT3/FLK-2-RECEPTOR
21
1.6.1
4
EXPRESSION
OF
FLT3/FLK-2
IN
MURINE
AND
HUMAN
TISSUES
22
1.6.1.5
EXPRESSION
OF
FLT3/FLK-2
IN
HEMATOPOIETIC
CELL
LINES
AND
PRIMARY
HUMAN
LEUKEMIAS
23
PAGE
1.6.2
THE
FLT3/FLK-2-LIGAND
25
1.6.2
1
CLONING
OF
THE
FLT3/FLK-2-LIGAND
25
1.6.2.2
STRUCTURE
AND
ISOFORMS
OF
THE
FLT3/FLK-2-LIGAND
25
1.6.2.3
EXPRESSION
OF
FLT3/FLK-2-LIGAND
MRNA
IN
MURINE
AND
HUMAN
TISSUES
26
1.6.2.4
BIOLOGICAL
ACTIVITIES
OF
THE
FLT3/FLK-2
LIGAND
27
1.7
EXPRESSION
AND
FUNCTION
OF
THE
FLT3/FLK-2-LIGAND
WITHIN
THE
HEMATOPOIETIC
SYSTEN
27
1.7.1
LONG-TERM
RECONSTITUTING
STEM/PROGENITOR
CELLS
28
1.7.2
IN
VITRO
PROLIFERATIVE
EFFECTS
OF
THE
FLT3/FLK-2-LIGAND
ON
PRIMITIVE
HEMATOPOIETIC
STEM/PROGENITOR
CELLS
29
1.7.3
COMMITTED
LYMPHOID
PROGENITORS
AND
PRECURSORS
30
1.7.4
COMMITTED
MYELOID
PROGENITORS
AND
PRECURSORS
32
1.7.5
MATURE
BLOOD
CELLS
33
2.
RATIONALS
34
3.
MATERIALS
AND
METHODS
35
3.1
MATERIALS
35
3.1.1
EQUIPMENT
35
3.1.2
MEMBRANES,
FILTERS,
PLASTIC
AND
GLASS
WARE
36
3.1.3
REAGENTS
36
3.1.4
BUFFERS
AND
SOLUTIONS
38
3.1.5
ENZYMES,
NUCLEOTIDES,
AND
PCR-PRIMERS
39
3.1.6
CELLS
AND
CELL
LINES
39
3.1.7
CELL
CULTURE
MEDIA
41
3.1.8
CYTOKINES
42
3.1.9 MONOCLONAL
ANTIBODIES
43
PAGE
3.2
METHODS
44
3.2.1
CELL
SEPARATION
AND
CELL
CULTURE
44
3.2.2
3
H-THYMIDINE
INCORPORATION
ASSAY
46
3.2.3
FLOW
CYTOMETRY 46
3.2.4
RNA
PREPARATION
47
3.2.5
REVERSE
TRANSCRIPTION
(RT)
AND
POLYMERASE 48
CHAIN
REACTION
(PCR)
3.2.6
CELL
LYSIS,
IMMUNOPRECIPITATION,
AND
WESTERN
BLOTTING
49
4.
RESULTS
53
4.1
PROLIFERATIVE
EFFECTS
OF
FLT3/FLK-2-LIGAND
ON
ALL
CELLS
53
4.2
DOSE-RESPONSE
AND
LONG-TERM
CULTURE
OF
ALL
CELLS
56
4.3
MORPHOLOGICAL
AND
IMMUNOPHENOTYPIC
ANALYSIS
OF
ALL
CELLS
58
4.4
FLT3/FLK-2
MRNA
EXPRESSION
IN
PRIMARY
ALL
CELLS
59
4.5
SIGNAL
TRANSDUCTION
OF
FLT3/FLK-2
IN
PRIMARY
ALL
CELLS
60
5.
DISCUSSION
64
5.1
PROLIFERATIVE
EFFECTS
OF
FLT3/FLK-2-LIGAND
ON
HEMATOPOIETIC
CELLS
64
5.2
EXPRESSION
OF
FLT3/FLK-2
MRNA
IN
NORMAL
AND
LEUKEMIC
CELLS
70
5.3
EXPRESSION
AND
FUNCTION
OF
FLT3/FLK-2-RECEPTORS
IN
LEUKEMIC
CELLS
73
5.4
CONCLUSIONS
74
6.
ABSTRACT
75
7.
REFERENCES
79
8.
DANKSAGUNG
108
9.
WIDMUNG
109
III
PAGE
10.
CURRICULUM
VITAE
110
11.
EHRENWORTLICHE
ERKLARUNG
112 |
| any_adam_object | 1 |
| author | Hemmati, Philipp Georg |
| author_facet | Hemmati, Philipp Georg |
| author_role | aut |
| author_sort | Hemmati, Philipp Georg |
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| building | Verbundindex |
| bvnumber | BV013233317 |
| ctrlnum | (OCoLC)76133148 (DE-599)BVBBV013233317 |
| discipline | Medizin |
| format | Book |
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| indexdate | 2024-08-19T00:20:10Z |
| institution | BVB |
| language | English |
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| physical | VII, 112 S. Ill., graph. Darst. : 21 cm |
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| spelling | Hemmati, Philipp Georg Verfasser aut Effects of Flt3, Flk2-ligand and Interleukin-7 on in vitro growth of acute lymphoblastic leukemia cells vorgelegt von Philipp Hemmati 1998 VII, 112 S. Ill., graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Frankfurt (Main), Univ., Diss., 2000 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009018426&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hemmati, Philipp Georg Effects of Flt3, Flk2-ligand and Interleukin-7 on in vitro growth of acute lymphoblastic leukemia cells |
| subject_GND | (DE-588)4113937-9 |
| title | Effects of Flt3, Flk2-ligand and Interleukin-7 on in vitro growth of acute lymphoblastic leukemia cells |
| title_auth | Effects of Flt3, Flk2-ligand and Interleukin-7 on in vitro growth of acute lymphoblastic leukemia cells |
| title_exact_search | Effects of Flt3, Flk2-ligand and Interleukin-7 on in vitro growth of acute lymphoblastic leukemia cells |
| title_full | Effects of Flt3, Flk2-ligand and Interleukin-7 on in vitro growth of acute lymphoblastic leukemia cells vorgelegt von Philipp Hemmati |
| title_fullStr | Effects of Flt3, Flk2-ligand and Interleukin-7 on in vitro growth of acute lymphoblastic leukemia cells vorgelegt von Philipp Hemmati |
| title_full_unstemmed | Effects of Flt3, Flk2-ligand and Interleukin-7 on in vitro growth of acute lymphoblastic leukemia cells vorgelegt von Philipp Hemmati |
| title_short | Effects of Flt3, Flk2-ligand and Interleukin-7 on in vitro growth of acute lymphoblastic leukemia cells |
| title_sort | effects of flt3 flk2 ligand and interleukin 7 on in vitro growth of acute lymphoblastic leukemia cells |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009018426&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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