Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2011
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 133 S. Ill., graph. Darst. |
Internformat
MARC
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| 100 | 1 | |a Heinrich, Stefanie |d 1981- |e Verfasser |0 (DE-588)1024207986 |4 aut | |
| 245 | 1 | 0 | |a Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus |c vorgelegt von Stefanie Heinrich |
| 264 | 1 | |c 2011 | |
| 300 | |a XII, 133 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Tübingen, Univ., Diss., 2012 | ||
| 650 | 0 | 7 | |a Staphylococcus carnosus |0 (DE-588)4316001-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Penicillinbindungsprotein |0 (DE-588)4255851-7 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Staphylococcus carnosus |0 (DE-588)4316001-3 |D s |
| 689 | 0 | 1 | |a Penicillinbindungsprotein |0 (DE-588)4255851-7 |D s |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-025216693 | ||
Datensatz im Suchindex
| _version_ | 1804149408675135490 |
|---|---|
| adam_text | IMAGE 1
INDEX
INDEX OF CONTENT
I INTRODUCTION 1
1.1 STAPHYLOCOCCI . 1
1.1.1 STAPHYLOCOCCUS AUREUS ABC 1
1.1.2 STAPHYLOCOCCUS CAMOSUS ABC 2
1.2 PEPTIDOGLYCAN ( MUREIN) 3
1.2.1 STRUCTURE 3
1.2.2 BIOSYNTHESIS 5
1.2.3 INTEGRATION OF NEW PEPTIDOGLYCAN 11
1.2.4 DEGRADATION OF PEPTIDOGLYCAN 15
1.3 PENICILLIN BINDING PROTEINS 18
1.3.1 S. AUREUS PBPS 21
1.3.2 PBPS OF S. CARNOSUS 23
1.4 SS-LACTAM ANTIBIOTICS 24
1.4.1 STRUCTURE AND FUNCTION 25
1.5 SS-LACTAM RESISTANCE 25
I.5.1 SS LACTAMASES 26
1.6 AIM OF THIS WORK 29
II MATERIAL AND METHODS 30
11.1 MATERIAL 30
II.1.1 CHEMICALS 30
11.1.2 INSTRUMENTS 32
11.1.3 OTHER MATERIALS 34
11.1.4 SOFTWARE 34
11.1.5 BIOMOLECULAR KITS 35
11.1.6 ENZYMES 36
11.1.7 DNA/PROTEIN ELECTROPHORESIS 36
11.1.8 ANTIBIOTICS 37
I
HTTP://D-NB.INFO/1024827313
IMAGE 2
INDEX
11.1.9 ANTIBODIES 37
11.1.10 BACTERIAL STRAINS 38
11.1.11 SYNTHETIC OLIGONUCLEOTIDES FOR PCR CLONING 39
11.1.12 SYNTHETIC OLIGONUCLEOTIDES FOR NORTHERN BLOTTING 41
11.1.13 SYNTHETIC OLIGONUCLEOTIDES FOR DNA SEQUENCING 41
11.1.14 PLASMIDS 42
11.1.15 VECTOR MAPS 43
11.2 METHODS 44
11.2.1 MICROBIAL METHODS 44
11.2.2 METHODS OF MOLECULAR BIOLOGY 46
11.2.3 METHODS FOR WORKING WITH PROTEINS 55
11.2.4 METHODS FOR FINDING PROTEIN-PROTEIN INTERACTIONS 68
11.3 METHODS FOR THE CHARACTERIZATION OF APBP3 71
11.3.1 E-TEST (EPSILOMETER TEST) 71
11.3.2 MIC-TEST 71
11.3.3 NORTHERN BLOT 72
III RESULTS 78
111.1 CLONING OF PBPS WITH N-TERMINAL HIS 6-TAG 78
111.2 CLONING AND OVEREXPRESSION OF PBPS WITH C-TERMINAL HIS 6-TAG 80
111.2.1 OVEREXPRESSION AND PURIFICATION OF PBP1 82
111.2.2 OVEREXPRESSION AND PURIFICATION OF PBP2 85
111.2.3 OVEREXPRESSION AND PURIFICATION OF PBP3 90
111.2.4 CLONING AND OVEREXPRESSION OF PBP4 92
111.2.5 CLONING OF PBP 4 WITH GST-TAG 96
111.2.6 BINDING ASSAYS WITH THE PBPS 97
111.2.7 PURIFICATION OF THE ANTIBODIES 98
111.2.8 TESTING OF THE ANTIBODIES 99
111.2.9 IMMUNOPRECIPITATION 100
111.2.10 CROSS-IINKING 104
111.2.11 CHARACTERIZATION OF A PBP3 108
II
IMAGE 3
INDEX
IV DISCUSSION 113
IV.1 OVEREXPRESSION AND PURIFICATION OF THE PBPS FROM S. CARNOSUS 113
IV.2 ACTIVITY TESTS 115
IV.3 INTERACTION STUDIES 116
V CONCLUSION AND OUTLOOK 119
VI REFERENCES 120
VII APPENDIX 129
VII.1.1 SEQUENCE OF P B P L (SCA_0794) ENCODING FOR PBP1 OF S.CARNOSUS
TM300 129
VII.1.2 SEQUENCE OF PBP2 (SCA_1084) ENCODING FOR PBP2 OF S.CARNOSUS
TM300
130
VII.1.3 SEQUENCE OF PBP3 (SCA_1173) ENCODING FOR PBP3 OF S.CARNOSUS
TM300
131
VII.1.4 SEQUENCE OF PBP4 (SCA_0291) ENCODING FOR PBP4 OF S.CARNOSUS
TM300
132
III
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| any_adam_object | 1 |
| author | Heinrich, Stefanie 1981- |
| author_GND | (DE-588)1024207986 |
| author_facet | Heinrich, Stefanie 1981- |
| author_role | aut |
| author_sort | Heinrich, Stefanie 1981- |
| author_variant | s h sh |
| building | Verbundindex |
| bvnumber | BV040362879 |
| ctrlnum | (OCoLC)815887241 (DE-599)BSZ368143791 |
| dewey-full | 610 570 |
| dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
| dewey-ones | 610 - Medicine and health 570 - Biology |
| dewey-raw | 610 570 |
| dewey-search | 610 570 |
| dewey-sort | 3610 |
| dewey-tens | 610 - Medicine and health 570 - Biology |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| illustrated | Illustrated |
| indexdate | 2024-07-10T00:22:30Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025216693 |
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| physical | XII, 133 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
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| spelling | Heinrich, Stefanie 1981- Verfasser (DE-588)1024207986 aut Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus vorgelegt von Stefanie Heinrich 2011 XII, 133 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Tübingen, Univ., Diss., 2012 Staphylococcus carnosus (DE-588)4316001-3 gnd rswk-swf Penicillinbindungsprotein (DE-588)4255851-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Staphylococcus carnosus (DE-588)4316001-3 s Penicillinbindungsprotein (DE-588)4255851-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025216693&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Heinrich, Stefanie 1981- Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus Staphylococcus carnosus (DE-588)4316001-3 gnd Penicillinbindungsprotein (DE-588)4255851-7 gnd |
| subject_GND | (DE-588)4316001-3 (DE-588)4255851-7 (DE-588)4113937-9 |
| title | Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus |
| title_auth | Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus |
| title_exact_search | Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus |
| title_full | Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus vorgelegt von Stefanie Heinrich |
| title_fullStr | Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus vorgelegt von Stefanie Heinrich |
| title_full_unstemmed | Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus vorgelegt von Stefanie Heinrich |
| title_short | Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus |
| title_sort | purification and investigation of the penicillin binding proteins of staphylococcus carnosus |
| topic | Staphylococcus carnosus (DE-588)4316001-3 gnd Penicillinbindungsprotein (DE-588)4255851-7 gnd |
| topic_facet | Staphylococcus carnosus Penicillinbindungsprotein Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025216693&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT heinrichstefanie purificationandinvestigationofthepenicillinbindingproteinsofstaphylococcuscarnosus |