Screening for human papillomavirus DNA in head and neck tumors, molecular cloning and characterization of HPV72 and HPV73 genomes:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
1994
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Heidelberg, Univ., Diss., 1995 |
| Beschreibung: | 108 Bl. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV010174400 | ||
| 003 | DE-604 | ||
| 007 | t | ||
| 008 | 950503s1994 gw ad|| m||| 00||| ger d | ||
| 016 | 7 | |a 943940001 |2 DE-101 | |
| 035 | |a (OCoLC)634713277 | ||
| 035 | |a (DE-599)BVBBV010174400 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a ger | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-11 | ||
| 100 | 1 | |a He, Yukai |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Screening for human papillomavirus DNA in head and neck tumors, molecular cloning and characterization of HPV72 and HPV73 genomes |c submitted by Yukai He |
| 264 | 1 | |c 1994 | |
| 300 | |a 108 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Heidelberg, Univ., Diss., 1995 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006757685&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-006757685 | |
Datensatz im Suchindex
| _version_ | 1812453842545016832 |
|---|---|
| adam_text |
CONTENTS
ABBREVIATIONS
1
1. INTRODUCTION 3
1.1. HPV INFECTION AND HUMAN DISEASES 3
1.1.1. ASSOCIATION OF HPV INFECTION WITH GENITAL TUMORS 3
1.1.2. ASSOCIATION OF HPV WITH THE TUMORS OF THE HEAD AND NECK
REGION AND UPPER RESPIRATORY TRACT
4
1.2. HPV GENOME ORGANISATION 6
1.2.1. THE LONG CONTROL REGION
7
1.2.2. EARLY ORFS
8
1.2.3. LATE ORFS
12
1.3. METHODS FOR DETECTING HPV DNA IN BIOPSIES 13
1.4. RESEARCH AIMS AND STRATEGY 1
8
2. MATERIALS AND METHODS
2.1. MATERIALS
17
2.2. METHODS
31
2.2.1 UNIVERSAL PCR
31
2.2.2 THERMAL CYCLE DIDEOXY SEQUENCING 35
2.2.3. POLYACRLAMIDE SEQUENCE GEL ELECTROPHORESIS 36
2.2.4. CLONING AND SEQUENCING OF THE PCR FRAGMENT 36
2.2.5. SEQUENCE COMPARISON WITH THE EXISTING DATABANK 40
2.2.6. CLONING OF PUTATIVE NEW HPV GENOMES 40
2.2.7. CHARACTERIZATION OF THE CLONED HPV GENOMES 45
3. RESULTS
49
3.1. SCREENING OF HPV DNA IN THE TUMORS OF HEAD AND NECK REGION 49
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/943940001
3.1.1. SCREENING RESULTS WITH PCR 50
3.1.2. DIRECT CYCLE SEQUENCING OF THE PCR PRODUCTS 52
3.1.3. CLONING AND SEQUENCING OF THE PCR PRODUCTS 52
3.1.4. SEQUENCE COMPARISON WITH DATABANK 53
3.1.5. SCREENING BY SOUTHERN BLOT HYBRIDIZATION 57
3.2. CLONING AND CHARACTERIZATION OF TWO NEW HPV GENOMES 57
3.2.1. CLONING OF HPV73 GENOME FROM SAMPLE WV-8653 58
3.2.2. CLONING OF HPV72 GENOME FROM SAMPLE WV-8652 62
3.2.3. CHARACTERIZATION OF THE TWO PUTATIVELY NEW HPV GENOMES 66
3.2.4. TRANSPOSITION OF THE TRANSPOSON IS5 INTO HPV72 GENOME 81
4. DISCUSSION ~
86
4
1
PRESENCE OF HPV DNA IN THE HEAD AND NECK TUMORS 86
4.1.1. HPV DNA IN THE BENIGN TUMORS OF HEAD AND NECK 87
4.1.2. HPV DNA IN THE MALIGNANT HEAD AND NECK TUMORS 88
4.1.3. DISCREPANCY BETWEEN THE RESULTS OF PCR AMPLIFICATION AND
SOUTHERN BLOT HYBRIDIZATION 90
4.2. CHARACTERIZATION OF HPV72 AND HPV73 DNA GENOMES 91
4.3. RECOMBINATION OF HPV DNA WITH BACTERIAL JUMPING GENE DURING
AMPLIFICATION OF HPV DNA IN BACTERIA 93
5. SUMMARY
95
6. REFERENCES
97
7. ACKNOWLEDGEMENT
108 |
| any_adam_object | 1 |
| author | He, Yukai |
| author_facet | He, Yukai |
| author_role | aut |
| author_sort | He, Yukai |
| author_variant | y h yh |
| building | Verbundindex |
| bvnumber | BV010174400 |
| ctrlnum | (OCoLC)634713277 (DE-599)BVBBV010174400 |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV010174400</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">950503s1994 gw ad|| m||| 00||| ger d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">943940001</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)634713277</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV010174400</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">ger</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-11</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">He, Yukai</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Screening for human papillomavirus DNA in head and neck tumors, molecular cloning and characterization of HPV72 and HPV73 genomes</subfield><subfield code="c">submitted by Yukai He</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1994</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">108 Bl.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Heidelberg, Univ., Diss., 1995</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006757685&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-006757685</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV010174400 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T16:17:58Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006757685 |
| oclc_num | 634713277 |
| open_access_boolean | |
| owner | DE-11 |
| owner_facet | DE-11 |
| physical | 108 Bl. Ill., graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| record_format | marc |
| spelling | He, Yukai Verfasser aut Screening for human papillomavirus DNA in head and neck tumors, molecular cloning and characterization of HPV72 and HPV73 genomes submitted by Yukai He 1994 108 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Heidelberg, Univ., Diss., 1995 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006757685&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | He, Yukai Screening for human papillomavirus DNA in head and neck tumors, molecular cloning and characterization of HPV72 and HPV73 genomes |
| subject_GND | (DE-588)4113937-9 |
| title | Screening for human papillomavirus DNA in head and neck tumors, molecular cloning and characterization of HPV72 and HPV73 genomes |
| title_auth | Screening for human papillomavirus DNA in head and neck tumors, molecular cloning and characterization of HPV72 and HPV73 genomes |
| title_exact_search | Screening for human papillomavirus DNA in head and neck tumors, molecular cloning and characterization of HPV72 and HPV73 genomes |
| title_full | Screening for human papillomavirus DNA in head and neck tumors, molecular cloning and characterization of HPV72 and HPV73 genomes submitted by Yukai He |
| title_fullStr | Screening for human papillomavirus DNA in head and neck tumors, molecular cloning and characterization of HPV72 and HPV73 genomes submitted by Yukai He |
| title_full_unstemmed | Screening for human papillomavirus DNA in head and neck tumors, molecular cloning and characterization of HPV72 and HPV73 genomes submitted by Yukai He |
| title_short | Screening for human papillomavirus DNA in head and neck tumors, molecular cloning and characterization of HPV72 and HPV73 genomes |
| title_sort | screening for human papillomavirus dna in head and neck tumors molecular cloning and characterization of hpv72 and hpv73 genomes |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006757685&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT heyukai screeningforhumanpapillomavirusdnainheadandnecktumorsmolecularcloningandcharacterizationofhpv72andhpv73genomes |