Basic bioscience laboratory techniques: a pocket guide
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Oxford [u.a.]
Wiley-Blackwell
2011
|
| Ausgabe: | 1. publ. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIX, 212 S. Ill., graph. Darst. |
| ISBN: | 9780470743096 |
Internformat
MARC
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Datensatz im Suchindex
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|---|---|
| adam_text | Basic Bioscience
Laboratory Techniques
A Pocket Guide
Philip Bonner and Alan Hargreaves
WILEY-BLACKWELL
A John Wiley amp; Sons, Ltd , Publication
CONTENTS
Preface ix
Glossary xi
Abbreviations xvii
1 The preparation of solutions in bioscience research 1
1 1 Introduction 1
1 2 Concentration 2
1 3 Using balances to weigh out reagents 3
1 4 Practical considerations when making a10M solution 6
1 5 Dilutions and the use of pipettes 11
1 6 Water, acids and bases 15
1 7 Buffers 21
1 8 The equilibrium/dissociation constant (Ka) for an acid
or base and the Henderson-Hasselbalch equation 24
1 9 Summary 26
2 Microscopy 27
2 1 Introduction 27
2 2 Microscopes - general principles 29
2 3 Principles of image formation 30
2 4 Light microscopy 32
2 5 Electron microscopy 46
2 6 Summary 49
3 Spectrophotometry 51
3 1 Introduction 51
3 2 The electromagnetic spectrum 51
3 3 The absorbance of light 54
3 4 Absorbtion spectrophotometry 56
3 5 The laws of the absorbance of light 57
3 6 The Beer Lambert law 61
3 7 Spectrophotometers 63
3 8 Applications of spectrophotometry in bioscience 68
3 9 Summary 73
CONTENTS
4 Data analysis and presentation 74
4 1 Introduction 74
4 2 Statistical analysis of data: some key definitions 76
4 3 Distributions 79
4 4 Statistical comparison of data 85
4 5 Presentation, structure and organization of data
in laboratory reports 94
4 6 Summary 100
5 The extraction and clarification of biological material 103
5 1 General introduction 103
5 2 Extraction 103
5 3 Extraction methods for animal and plant tissue 105
5 4 Extraction methods for bacteria 107
5 5 Clarification 108
5 6 Centrifugation techniques 115
5 7 Points of good practice in centrifugation 119
5 8 Summary 120
6 Electrophoresis of proteins and nucleic acids 121
6 1 General introduction 121
6 2 Separation of protein mixtures by gel electrophoresis 122
6 3 Other electrophoretic techniques applied to proteins 136
6 4 Separation of nucleic acids by gel electrophoresis 137
6 5 Applications of gel electrophoresis of nucleic acids 141
6 6 Summary 146
7 Chromatography 147
7 1 General introduction 147
7 2 The theory of chromatography 147
7 3 Factors to consider in chromatography 152
7 4 Methods used to elute samples in chromatography 153
7 5 Different types of chromatography and what properties
can be used to separate molecules 153
7 6 Thin layer chromatography (TLC) 156
7 7 High pressure liquid chromatography (HPLC) 158
7 8 Gas liquid chromatography (GLC) 161
7 9 Ion exchange chromatography (IEX) 162
7 10 Size exclusion chromatography 164
7 11 Affinity chromatography 166
7 12 Summary 167
VI
CONTENTS
8 Cell culture techniques 169
8 1 Introduction 169
8 2 Growth and maintenance of cells in culture 174
8 3 Summary 192
Suggestions for further reading 195
Index 198
vn
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| spelling | Bonner, Philip Verfasser aut Basic bioscience laboratory techniques a pocket guide Philip Bonner and Alan Hargreaves 1. publ. Oxford [u.a.] Wiley-Blackwell 2011 XIX, 212 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biology Laboratory manuals Biology Handbooks, manuals, etc Laboratory Techniques and Procedures Handbooks Biotechnology Handbooks Research Handbooks Biowissenschaften (DE-588)4129772-6 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf (DE-588)4151278-9 Einführung gnd-content Biowissenschaften (DE-588)4129772-6 s Labortechnik (DE-588)4123602-6 s DE-604 Hargreaves, Alan 1933- Verfasser (DE-588)140636927 aut HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021119219&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bonner, Philip Hargreaves, Alan 1933- Basic bioscience laboratory techniques a pocket guide Biology Laboratory manuals Biology Handbooks, manuals, etc Laboratory Techniques and Procedures Handbooks Biotechnology Handbooks Research Handbooks Biowissenschaften (DE-588)4129772-6 gnd Labortechnik (DE-588)4123602-6 gnd |
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| title | Basic bioscience laboratory techniques a pocket guide |
| title_auth | Basic bioscience laboratory techniques a pocket guide |
| title_exact_search | Basic bioscience laboratory techniques a pocket guide |
| title_full | Basic bioscience laboratory techniques a pocket guide Philip Bonner and Alan Hargreaves |
| title_fullStr | Basic bioscience laboratory techniques a pocket guide Philip Bonner and Alan Hargreaves |
| title_full_unstemmed | Basic bioscience laboratory techniques a pocket guide Philip Bonner and Alan Hargreaves |
| title_short | Basic bioscience laboratory techniques |
| title_sort | basic bioscience laboratory techniques a pocket guide |
| title_sub | a pocket guide |
| topic | Biology Laboratory manuals Biology Handbooks, manuals, etc Laboratory Techniques and Procedures Handbooks Biotechnology Handbooks Research Handbooks Biowissenschaften (DE-588)4129772-6 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Biology Laboratory manuals Biology Handbooks, manuals, etc Laboratory Techniques and Procedures Handbooks Biotechnology Handbooks Research Handbooks Biowissenschaften Labortechnik Einführung |
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