Planar lipid bilayers: methods and applications
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
London
Academic Press
©1993
|
| Schriftenreihe: | Biological techniques series
|
| Schlagworte: | |
| Online-Zugang: | FAW01 Volltext |
| Beschreibung: | "Key references": p. [129]-130 Includes bibliographical references and index Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers. In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series. The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work. Methods of constructing artificial membranes (planar lipid bilayers) from the main components of cell membranes (lipids) date from the early 1960s. Planar bilayers offer direct, quantitative experimental approaches to the study of membranes of precisely determined composition which can be manipulated by the experimenter. Pore-forming molecules, transporter molecules, ATP-dependent enzymes and other entities can be incorporated into the bilayers to simulate biological functions. Reconstitution of such functions in this way remains a key final step in attributing a functional role to purified cell membrane proteins. This book aims to demystify these techniques and begins with a broad overview of the development of the subject before dealing with the protocols involved. Key references are provided at the end of the book together with a list of suppliers. Full practical details include: * How to set up conventional painted and folded bilayer experiments * Patch-dipping methods and use of giant liposomes * Lipid characterization, preparation and purification * Discussion of construction of essential apparatus, flux measurements, electrical recording, data acquisition and computer support * Biochemical methods for use in planar bilayer experiments * Techniques for incorporation of native proteins and other molecules |
| Beschreibung: | 1 Online-Ressource (x, 133 pages) |
| ISBN: | 0123229952 1299195555 9780123229953 9781299195554 |
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| 500 | |a Planar bilayers offer direct, quantitative experimental approaches to the study of membranes of precisely determined composition which can be manipulated by the experimenter. Pore-forming molecules, transporter molecules, ATP-dependent enzymes and other entities can be incorporated into the bilayers to simulate biological functions. Reconstitution of such functions in this way remains a key final step in attributing a functional role to purified cell membrane proteins. This book aims to demystify these techniques and begins with a broad overview of the development of the subject before dealing with the protocols involved. Key references are provided at the end of the book together with a list of suppliers. | ||
| 500 | |a Full practical details include: * How to set up conventional painted and folded bilayer experiments * Patch-dipping methods and use of giant liposomes * Lipid characterization, preparation and purification * Discussion of construction of essential apparatus, flux measurements, electrical recording, data acquisition and computer support * Biochemical methods for use in planar bilayer experiments * Techniques for incorporation of native proteins and other molecules | ||
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Datensatz im Suchindex
| _version_ | 1804152883018465280 |
|---|---|
| any_adam_object | |
| author | Hanke, W., (Wolfgang) |
| author_facet | Hanke, W., (Wolfgang) |
| author_role | aut |
| author_sort | Hanke, W., (Wolfgang) |
| author_variant | w w h ww wwh |
| building | Verbundindex |
| bvnumber | BV042302808 |
| classification_rvk | WC 5100 WE 5150 |
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| ctrlnum | (OCoLC)669229327 (DE-599)BVBBV042302808 |
| dewey-full | 660/.6 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660/.6 |
| dewey-search | 660/.6 |
| dewey-sort | 3660 16 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie Biologie |
| format | Electronic eBook |
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| id | DE-604.BV042302808 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T01:17:47Z |
| institution | BVB |
| isbn | 0123229952 1299195555 9780123229953 9781299195554 |
| language | English |
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| physical | 1 Online-Ressource (x, 133 pages) |
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| publishDate | 1993 |
| publishDateSearch | 1993 |
| publishDateSort | 1993 |
| publisher | Academic Press |
| record_format | marc |
| series2 | Biological techniques series |
| spelling | Hanke, W., (Wolfgang) Verfasser aut Planar lipid bilayers methods and applications W. Hanke, W.-R. Schlue London Academic Press ©1993 1 Online-Ressource (x, 133 pages) txt rdacontent c rdamedia cr rdacarrier Biological techniques series "Key references": p. [129]-130 Includes bibliographical references and index Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers. In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series. The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work. Methods of constructing artificial membranes (planar lipid bilayers) from the main components of cell membranes (lipids) date from the early 1960s. Planar bilayers offer direct, quantitative experimental approaches to the study of membranes of precisely determined composition which can be manipulated by the experimenter. Pore-forming molecules, transporter molecules, ATP-dependent enzymes and other entities can be incorporated into the bilayers to simulate biological functions. Reconstitution of such functions in this way remains a key final step in attributing a functional role to purified cell membrane proteins. This book aims to demystify these techniques and begins with a broad overview of the development of the subject before dealing with the protocols involved. Key references are provided at the end of the book together with a list of suppliers. Full practical details include: * How to set up conventional painted and folded bilayer experiments * Patch-dipping methods and use of giant liposomes * Lipid characterization, preparation and purification * Discussion of construction of essential apparatus, flux measurements, electrical recording, data acquisition and computer support * Biochemical methods for use in planar bilayer experiments * Techniques for incorporation of native proteins and other molecules Lipid Bilayers Bilayer lipid membranes / Biotechnology fast Lipid membranes / Biotechnology fast Bilayer lipid membranes Biotechnology Lipid membranes Biotechnology Lipide (DE-588)4035873-2 gnd rswk-swf Doppelschicht (DE-588)4150451-3 gnd rswk-swf Lipide (DE-588)4035873-2 s Doppelschicht (DE-588)4150451-3 s 1\p DE-604 Schlue, W.-R. Sonstige oth Erscheint auch als Druck-Ausgabe, Hardcover 0-12-322994-4 Erscheint auch als Druck-Ausgabe, Hardcover 978-0-12-322994-6 http://www.sciencedirect.com/science/book/9780123229953 Verlag Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Hanke, W., (Wolfgang) Planar lipid bilayers methods and applications Lipid Bilayers Bilayer lipid membranes / Biotechnology fast Lipid membranes / Biotechnology fast Bilayer lipid membranes Biotechnology Lipid membranes Biotechnology Lipide (DE-588)4035873-2 gnd Doppelschicht (DE-588)4150451-3 gnd |
| subject_GND | (DE-588)4035873-2 (DE-588)4150451-3 |
| title | Planar lipid bilayers methods and applications |
| title_auth | Planar lipid bilayers methods and applications |
| title_exact_search | Planar lipid bilayers methods and applications |
| title_full | Planar lipid bilayers methods and applications W. Hanke, W.-R. Schlue |
| title_fullStr | Planar lipid bilayers methods and applications W. Hanke, W.-R. Schlue |
| title_full_unstemmed | Planar lipid bilayers methods and applications W. Hanke, W.-R. Schlue |
| title_short | Planar lipid bilayers |
| title_sort | planar lipid bilayers methods and applications |
| title_sub | methods and applications |
| topic | Lipid Bilayers Bilayer lipid membranes / Biotechnology fast Lipid membranes / Biotechnology fast Bilayer lipid membranes Biotechnology Lipid membranes Biotechnology Lipide (DE-588)4035873-2 gnd Doppelschicht (DE-588)4150451-3 gnd |
| topic_facet | Lipid Bilayers Bilayer lipid membranes / Biotechnology Lipid membranes / Biotechnology Bilayer lipid membranes Biotechnology Lipid membranes Biotechnology Lipide Doppelschicht |
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