Macromolecules and their interactions of the surface of native cartilage fibrils:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
Aachen
Shaker
1997
|
| Ausgabe: | Als Ms. gedr. |
| Schriftenreihe: | Berichte aus der Chemie
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zugl.: Münster (Westfalen), Univ., Diss., 1997 |
| Beschreibung: | VI, 108 S. Ill., graph. Darst. |
| ISBN: | 3826529553 |
Internformat
MARC
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Datensatz im Suchindex
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|---|---|
| adam_text | Table of Contents
Acknowledgements iv
Preface v
Abbreviations vi
I General Introduction 1
1 Cartilage 2
1.1 Hyaline cartilage 3
1.1.1 Structure and function of epiphyseal cartilage 3
1.1.2 Structure and function of articular cartilage 7
2 Molecular constituents of cartilage 8
2.1 Collagens 8
2.1.1 Collagen H 12
2.1.2 Collagen XI 13
2.1.3 Collagen DC 14
2.1.4 Collagens Xn and XTV 16
2.1.5 Collagen X 17
2.1.6 Collagen VI 17
2.2 Proteoglycans 18
2.2.1 Large aggregating proteoglycans aggrecan and versican 18
2.2.2 Small interstitial CS/DS proteoglycans decorin and biglycan 19
2.2.3 Small interstitial KS proteoglycans fibromodulin and lumican 21
2.3 Other matrix proteins 22
2.3.1 Adhesive glycoproteins fibronectin, thrombospondin, and chondroadherin 22
2.3.2 Other matrix constituents link protein, COMP, CMP, and PRELP 23
3 Macromolecular assemblies 26
3.1 D periodic fibrils 26
3.2 Proteoglycan aggregates 31
3.3 Other fibrillar aggregates 31
i
II Present Investigation 35
4 Differential distribution of decorin and collagen IX among articular cartilage
fibrils 38
4.1 Introduction 38
4.2 Material and methods 40
4.2.1 Tissues and source preparation 40
4.2.2 Isolation and separation of cartilage collagens by pepsin digestion 40
4.2.3 Antisera to collagen IX and decorin 40
4.2.4 Enzyme linked immunosorbent assay (ELISA) 40
4.2.5 SDS PAGE and immunoblotting 41
4.2.6 Extraction of fibril fragments 42
4.2.7 Electron microscopy 42
4.2.8 Quantitation of collagens in cartilage tissue and extracted fibrils 43
4.2.9 Measurement of fibril dimensions and fibrillar labeling 43
4.3 Results 44
4.3.1 Extraction of fibril fragments 44
4.3.2 Localization of decorin on the surface of cartilage fibrils 47
4.3.3 Characterization of the antiserum to bovine collagen IX 47
4.3.4 Immunoelectron microscopic localization of collagen IX 51
4.3.5 Comparison of fibril populations containing decorin and collagen K 51
4.3.6 Rotary shadowing of cartilage fibrils 52
4.4 Discussion 58
5 Absence of the al(IX) chain leads to a functional knock out of entire collagen IX
protein in mice 62
5.1 Introduction 62
5.2 Material and methods 64
5.2.1 Experimental animals and cartilage preparation 64
5.2.2 RNA extraction and RNA hybridization 64
5.2.3 Antibodies to collagens DC and II 64
5.2.4 Extraction of fibril fragments, collagens, and soluble molecules 65
5.2.5 Measurement of fibril diameters and labeled fibrils 65
5.3 Results 66
5.3.1 Expression of the Col9a2 and Col9a3 genes in Col9al knock out mice 66
5.3.2 Ultrastructure of cartilage fibrils 67
5.4 Discussion 71
6 Collagen X a constituent of fibrils in hypertrophic cartilage? 73
6.1 Introduction 73
6.2 Material and methods 75
6.2.1 Materials 75
6.2.2 Preparation and purification of native collagen X 75
6.2.3 Preparation and purification of native collagens n, IX, and XI 75
6.2.4 Enzymatic digestions 76
6.2.5 Antibodies 76
6.2.6 Solid phase assay 76
6.3 Results 77
6.3.1 Antibodies to collagen X 77
6.3.2 Immunoelectron microscopy of fibril fragments 79
6.3.3 Biochemical characterization of fibril fragments 82
6.3.4 Reconstitution experiments with collagen X 84
6.4 Discussion 84
HI Summary Zusammenfassung 87
IV References 93
iii
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| any_adam_object | 1 |
| author | Hagg, Rupert 1965- |
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| bvnumber | BV011534564 |
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| ctrlnum | (OCoLC)75784050 (DE-599)BVBBV011534564 |
| discipline | Biologie |
| edition | Als Ms. gedr. |
| format | Book |
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| id | DE-604.BV011534564 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:11:23Z |
| institution | BVB |
| isbn | 3826529553 |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007763733 |
| oclc_num | 75784050 |
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| physical | VI, 108 S. Ill., graph. Darst. |
| publishDate | 1997 |
| publishDateSearch | 1997 |
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| publisher | Shaker |
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| series2 | Berichte aus der Chemie |
| spelling | Hagg, Rupert 1965- Verfasser (DE-588)115786937 aut Macromolecules and their interactions of the surface of native cartilage fibrils Rupert Hagg Als Ms. gedr. Aachen Shaker 1997 VI, 108 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Berichte aus der Chemie Zugl.: Münster (Westfalen), Univ., Diss., 1997 Fibrille (DE-588)4154296-4 gnd rswk-swf Grundsubstanz (DE-588)4224942-9 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf Wechselwirkung (DE-588)4064937-4 gnd rswk-swf Hyaliner Knorpel (DE-588)4266348-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Hyaliner Knorpel (DE-588)4266348-9 s Fibrille (DE-588)4154296-4 s Grundsubstanz (DE-588)4224942-9 s Proteine (DE-588)4076388-2 s Wechselwirkung (DE-588)4064937-4 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007763733&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hagg, Rupert 1965- Macromolecules and their interactions of the surface of native cartilage fibrils Fibrille (DE-588)4154296-4 gnd Grundsubstanz (DE-588)4224942-9 gnd Proteine (DE-588)4076388-2 gnd Wechselwirkung (DE-588)4064937-4 gnd Hyaliner Knorpel (DE-588)4266348-9 gnd |
| subject_GND | (DE-588)4154296-4 (DE-588)4224942-9 (DE-588)4076388-2 (DE-588)4064937-4 (DE-588)4266348-9 (DE-588)4113937-9 |
| title | Macromolecules and their interactions of the surface of native cartilage fibrils |
| title_auth | Macromolecules and their interactions of the surface of native cartilage fibrils |
| title_exact_search | Macromolecules and their interactions of the surface of native cartilage fibrils |
| title_full | Macromolecules and their interactions of the surface of native cartilage fibrils Rupert Hagg |
| title_fullStr | Macromolecules and their interactions of the surface of native cartilage fibrils Rupert Hagg |
| title_full_unstemmed | Macromolecules and their interactions of the surface of native cartilage fibrils Rupert Hagg |
| title_short | Macromolecules and their interactions of the surface of native cartilage fibrils |
| title_sort | macromolecules and their interactions of the surface of native cartilage fibrils |
| topic | Fibrille (DE-588)4154296-4 gnd Grundsubstanz (DE-588)4224942-9 gnd Proteine (DE-588)4076388-2 gnd Wechselwirkung (DE-588)4064937-4 gnd Hyaliner Knorpel (DE-588)4266348-9 gnd |
| topic_facet | Fibrille Grundsubstanz Proteine Wechselwirkung Hyaliner Knorpel Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007763733&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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