4-1BB overexpression in basal keratinocyte induces macrophage infiltration, epithelial-mesenchymal transition and anterior cataract in the eye:
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Münster
2019
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| Beschreibung: | 85, XLIX Blätter Illustrationen 30 cm |
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MARC
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| 100 | 1 | |a Gonnelli, Elena |d 1990- |e Verfasser |0 (DE-588)1195239211 |4 aut | |
| 245 | 1 | 0 | |a 4-1BB overexpression in basal keratinocyte induces macrophage infiltration, epithelial-mesenchymal transition and anterior cataract in the eye |c vorgelegt von Elena Gonnelli |
| 264 | 1 | |a Münster |c 2019 | |
| 300 | |a 85, XLIX Blätter |b Illustrationen |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Westfälische-Wilhelms-Universität Münster |d 2019 |g Beschränkt für den Austausch | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-031717569 | ||
Datensatz im Suchindex
| _version_ | 1804180838978420736 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
1
INTRODUCTION
..........................................................................................................................
1
1.
1
E
YE
:
A
VERY
COMPLEX
AND
DELICATE
ORGAN
..........................................................................
1
1.1.1
OVERVIEW
ON
EYE
ANATOMY
....................................................................................................
1
1.1.2
EYE
TISSUE-RESIDENT
IMMUNITY
..............................................................................................
3
1.1.3
EYE
IMMUNE-PRIVILEGE
..........................................................................................................
5
1.1.4
EYE
INFLAMMATIONS
.................................................................................................................
7
1.1.5
CATARACT:
THE
LEADING
CAUSE
OF
BLINDNESS
.............................................................................
8
1.2
E
PITHELIAL
-
MESENCHYMAL
TRANSITION
...................................................................................
9
1.2.1
TGF-0
SIGNALING
IN
EMT
....................................................................................................
10
1.2.2
EMT
IN
EYE
TISSUE
..................................................................................................................
11
1.3
A
TOPIC
DERMATITIS
AND
EYE
PATHOLOGY
..............................................................................
12
1.4
4-1
BB/4-
1
BBL
SIGNALING
....................................................................................................................................
13
1.5
O
BJECTIVES
....................................................................................................................................
17
2
MATERIALS
&
METHODS
.......................................................................................................
18
2.1
M
ATERIALS
....................................................................................................................................
18
2.1.1
EQUIPMENTS
...........................................................................................................................
18
2.1.2
DISPOSABLES
...........................................................................................................................
19
2.1.3
CHEMICALS
&
KITS
.................................................................................................................
19
2.1.4
MEDIA
&
BUFFERS
...................................................................................................................
20
2.1.5
ANTIBODIES
...........................................................................................................................
21
2.1.6
SECONDARY
ANTIBODIES
...........................................................................................................
22
2.1.7
PRIMERS
..................................................................................................................................
22
2.1.8
MOUSE
LINES
..........................................................................................................................
23
2.1.9
SOFTWARE
................................................................................................................................
23
2.2
M
ETHODS
......................................................................................................................................
23
2.2.1
EXPERIMENTAL
ANIMALS
&
HOUSING
.......................................................................................
23
2.2.2
EVALUATION
OF
DISEASE
SEVERITY
...........................................................................................
24
2.2.3
MULTICOLOR
FLOW
CYTOMETRY
ANALYSES
OF
IMMUNE
CELLS
....................................................
24
2.2.4
HISTOLOGICAL
EXAMINATION
....................................................................................................
26
2.2.4.1
HEMATOXYLIN
&
EOSIN
STAINING
......................................................................................26
2.2.4.2
IMMUNOFLUORESCENCE
STAINING
.......................................................................................
26
2.2.5
QUANTIFICATION
OF
CYTOKINES
AND
CHEMOKINES
IN
TOTAL
EYE
PROTEIN
..................................
27
2.2.5.1
EXTRACTION
OF
TOTAL
EYE
PROTEIN
.......................................................................................
27
2.2.5.2
TOTAL
PROTEIN
QUANTIFICATION
BY
BCA
ASSAY
..................................................................
27
2.2.5.3
LEGENDPLEX*
.............................................................................................................
28
2.2.6
QUANTITATIVE
REAL-TIME
PCR
...............................................................................................
28
2.2.6.1
RNA
EXTRACTION
...............................................................................................................28
2.2.6.2
RNA
QUALITY
....................................................................................................................
29
2.2.6.3
RNA
REVERSE
TRANSCRIPTION
AND
QUANTITATIVE
PCR
...................................................... 29
2.2.7
MICROARRAY
ANALYSIS
............................................................................................................
29
2.2.8
STATISTICAL
ANALYSIS
...............................................................................................................
30
3
RESULTS
..........................................................................................................................................
31
3.1
KI
4-4-
IBB
TG
MICE
PHENOTYPE
EVALUATION
..........................................................................................
31
3.2
A
NALYSIS
OF
4-
IBB
EXPRESSION
..............................................................................................
33
3.3
T
IME
COURSE
ANALYSIS
OF
PHENOTYPE
DEVELOPMENT
........................................................
35
3.4
E
VALUATION
OF
THE
LENS
TISSUE
DISRUPTION
........................................................................36
3.5
KI
4-4-
IBB
TG
MICE
SHOW
MORPHOLOGICAL
SIGNS
OF
EYE
INFLAMMATION
..........................
44
3.6
I
NCREASED
NUMBERS
OF
LEUKOCYTES
IN
K
14-4-1
BB
TG
MICE
.......................................... 46
3.7
M
ACROPHAGES
PLAY
A
KEY
ROLE
IN
EYE
INFLAMMATION
OF
K
14-4-1
BB
TG
MICE
.........
51
3.8
M
ARGINAL
ROLE
OF
GRANULOCYTES
AND
T
CELL
..................................................................
56
3.9
I
NDUCTION
OF
EPITHELIAL
-
MESENCHYMAL
TRANSITION
IN
K
14-4-1
BB
TG
MICE
............. 60
3.10
I
NCREASED
EXPRESSION
OF
TGF-
B
2
.........................................................................................
63
4
DISCUSSION
...................................................................................................................................
67
4.1
KI
4-4-1
BB
TG
MICE,
A
MODEL
TO
INVESTIGATE
THE
CONNECTION
BETWEEN
ATOPIC
DERMATITIS
AND
EYE
PATHOLOGY
...............................................................................................................................
68
4.1.1
KI
4-4-
IBB
MICE
DEVELOP
A
CATARACT-LIKE
DISEASE
...........................................................69
4.2
KI
4-4-
IBB
TG
MICE
SHOW
CLEAR
SIGNS
OF
AN
EYE
INFLAMMATION
............................................
71
4.2.1
KI
4-4-
IBB
TG
MICE
SHOW
SIGNS
OF
A
POSSIBLE
INCREASED
PERMEABILITY
OF
THE
BLOOD-EYE
BARRIER
..................................................................................................................................................
73
4.2.2
MARGINAL
ROLE
OF
T
CELLS
AND
GRANULOCYTES
.......................................................................
75
4.2.3
MACROPHAGES
PLAY
A
KEY
ROLE
IN
KI
4-4-
IBB
TG
EYE
DISEASE
..........................................
76
4.3
M
ACROPHAGES
INDUCE
EMT
IN
THE
LENS
OF
K
14-4-1
BB
TG
MICE
....................................
78
4.3.1
THE
ROLE
OF
TGF-|32
IN
EMT
INDUCTION
IN
KI
4-4-
IBB
TG
MICE
....................................
81
5
CONCLUSIONS
AND
OUTLOOK
............................................................................................
83
LIST
OF
REFERENCES
.....................................................................................................................
XII
APPENDIX
........................................................................................................................................
XXXII
|
| any_adam_object | 1 |
| author | Gonnelli, Elena 1990- |
| author_GND | (DE-588)1195239211 |
| author_facet | Gonnelli, Elena 1990- |
| author_role | aut |
| author_sort | Gonnelli, Elena 1990- |
| author_variant | e g eg |
| building | Verbundindex |
| bvnumber | BV046340929 |
| ctrlnum | (OCoLC)1136986969 (DE-599)DNB1196231230 |
| discipline | Medizin |
| format | Thesis Book |
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| id | DE-604.BV046340929 |
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| indexdate | 2024-07-10T08:42:08Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031717569 |
| oclc_num | 1136986969 |
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| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 85, XLIX Blätter Illustrationen 30 cm |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| record_format | marc |
| spelling | Gonnelli, Elena 1990- Verfasser (DE-588)1195239211 aut 4-1BB overexpression in basal keratinocyte induces macrophage infiltration, epithelial-mesenchymal transition and anterior cataract in the eye vorgelegt von Elena Gonnelli Münster 2019 85, XLIX Blätter Illustrationen 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Westfälische-Wilhelms-Universität Münster 2019 Beschränkt für den Austausch (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1196231230/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031717569&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Gonnelli, Elena 1990- 4-1BB overexpression in basal keratinocyte induces macrophage infiltration, epithelial-mesenchymal transition and anterior cataract in the eye |
| subject_GND | (DE-588)4113937-9 |
| title | 4-1BB overexpression in basal keratinocyte induces macrophage infiltration, epithelial-mesenchymal transition and anterior cataract in the eye |
| title_auth | 4-1BB overexpression in basal keratinocyte induces macrophage infiltration, epithelial-mesenchymal transition and anterior cataract in the eye |
| title_exact_search | 4-1BB overexpression in basal keratinocyte induces macrophage infiltration, epithelial-mesenchymal transition and anterior cataract in the eye |
| title_full | 4-1BB overexpression in basal keratinocyte induces macrophage infiltration, epithelial-mesenchymal transition and anterior cataract in the eye vorgelegt von Elena Gonnelli |
| title_fullStr | 4-1BB overexpression in basal keratinocyte induces macrophage infiltration, epithelial-mesenchymal transition and anterior cataract in the eye vorgelegt von Elena Gonnelli |
| title_full_unstemmed | 4-1BB overexpression in basal keratinocyte induces macrophage infiltration, epithelial-mesenchymal transition and anterior cataract in the eye vorgelegt von Elena Gonnelli |
| title_short | 4-1BB overexpression in basal keratinocyte induces macrophage infiltration, epithelial-mesenchymal transition and anterior cataract in the eye |
| title_sort | 4 1bb overexpression in basal keratinocyte induces macrophage infiltration epithelial mesenchymal transition and anterior cataract in the eye |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1196231230/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031717569&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT gonnellielena 41bboverexpressioninbasalkeratinocyteinducesmacrophageinfiltrationepithelialmesenchymaltransitionandanteriorcataractintheeye |
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