Development and analysis of improved Polyethylenimine (PEI)-based nanoparticles for the delivery of therapeutic nucleic acids: in vitro and in vivo studies
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| Format: | Abschlussarbeit Elektronisch Software E-Book |
| Sprache: | English |
| Veröffentlicht: |
Leipzig
[2017]
|
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| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 1 CD-ROM (248 Blätter) Illustrationen, Diagramme |
Internformat
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| 245 | 1 | 0 | |a Development and analysis of improved Polyethylenimine (PEI)-based nanoparticles for the delivery of therapeutic nucleic acids |b in vitro and in vivo studies |c vorgelegt von Diplomchemiker Alexander Ewe |
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Datensatz im Suchindex
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|---|---|
| adam_text | CONTENT
SUMMARY................................................................................................................................
5
ZUSAMMENFASSUNG.................................................................................................................10
1 GENERAL
INTRODUCTION........................................................................................................15
1.1
NANOMEDICINE..........................................................................................................
15
1.2 THERAPEUTIC NUCLEIC
ACIDS.........................................................................................
16
1.3 NON-VIRAL
VECTORS.......................................................................................................
21
1.4 CATIONIC
POLYMERS.....................................................................................................24
1.5
POLYETHYLENIMINE......................................................................................................26
1.6 LIPOSOMES AND LIPID VECTORS
....................................................................................
34
1.7 CLINICAL
TRIALS............................................................................................................
41
1.8 STRUCTURE AND OBJECTIVES OF THIS
THESIS.......................................................................44
1.9
REFERENCES.................................................................................................................47
2 MATERIALS AND
METHODS....................................................................................................58
3 PREFACE TO
LIPOPOLYPLEXES.................................................................................................
79
4 STORAGE STABILITY OF OPTIMAL LIPOSOME-POLYETHYLENIMINE COMPLEXES
(LIPOPOLYPLEXES) FOR
DNA OR SIRNA
DELIVERY..................................................................................................
84
4.1
INTRODUCTION..............................................................................................................86
4.2 MATERIALS AND
METHODS.............................................................................................88
4.3
RESULTS......................................................................................................................
92
4.4 LIPOPOLYPLEX FORMATION PRESERVES BIOLOGICAL ACTIVITY UPON
STORAGE........................98
4.5
DISCUSSION...............................................................................................................103
4.6 SUPPLEMENTARY
INFORMATION....................................................................................105
4.7
REFERENCES...............................................................................................................107
5 NEBULIZATION OF LIPOSOME-POLYETHYLENIMINE COMPLEXES (LIPOPOLYPLEXES)
FOR DNA OR
SIRNA DELIVERY: PHYSICOCHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITY
............................
I L L
5.1
INTRODUCTION............................................................................................................
113
5.2 MATERIALS AND
METHODS...........................................................................................
114
5.3
DISCUSSION...............................................................................................................
127
5.4
REFERENCES...............................................................................................................
129
6 LIPOSOME-POLYETHYLENIMINE COMPLEXES (DPPC-PEI LIPOPOLYPLEXES) FOR
THERAPEUTIC
SIRNA DELIVERY IN
VIVO...................................................................................................
132
6.1
INTRODUCTION............................................................................................................
134
6.2 MATERIALS AND
METHODS...........................................................................................
136
6.3
RESULTS....................................................................................................................
142
6.4
DISCUSSION...............................................................................................................
149
6.5 SUPPLEMENTARY INFORMATION.
6.6 REFERENCES
...........................
153
154
7 A NOVEL TYROSINE-MODIFIED LOW MOLECULAR WEIGHT POLYETHYLENIMINE (P10Y)
FOR EFFICIENT
SIRNA DELIVERY IN VITRO AND IN
VIVO.................................................................................
158
7.1
INTRODUCTION............................................................................................................
160
7.2 MATERIALS AND
METHODS...........................................................................................
162
7.3
RESULTS....................................................................................................................
172
7.4
DISCUSSION...............................................................................................................
183
7.5 SUPPLEMENTARY
INFORMATION....................................................................................187
7.6
REFERENCES...............................................................................................................
191
8 TYROSINE MODIFICATION OF PEI: A COMPARISON BETWEEN POLYMER MOLECULAR
WEIGHT,
PHYSICOCHEMICAL PROPERTIES AND BIOLOGICAL
ACTIVITY........................................................195
8.1
INTRODUCTION............................................................................................................
195
8.2 MATERIALS AND
METHODS...........................................................................................
196
8.3 RESULTS AND
DISCUSSION............................................................................................196
8.4 CONCLUSION AND
OUTLOOK..........................................................................................
204
8.5
REFERENCES...............................................................................................................206
9 FINAL
DISCUSSION.............................................................................................................
208
9.1
REFERENCES...............................................................................................................218
10 SUPPLEMENTARY
DATA.......................................................................................................220
10.1 NMR SPECTRA OF TYROSINE-MODIFIED
PEIS........................................................... 220
10.2 SUPPLEMENTARY DATA ON DNA LIPOPOLYPLEXES
........................................................
222
10.3 SUPPLEMENTARY DATA ON SIRNA LIPOPOLYPLEXES
......................................................
224
11
ABBREVIATIONS................................................................................................................230
12
APPENDIX.......................................................................................................................
232
12.1
LEBENSLAUF...............................................................................................................
232
12.2 PEER-REVIEWED
PUBLICATIONS.....................................................................................234
12.3 BOOK
CHAPTER...........................................................................................................
235
12.4
PRESENTATIONS..........................................................................................................
235
12.5 PUBLISHED
ABSTRACTS.................................................................................................
236
13
SELBSTSTAENDIGKEITSERKLAERUNG...........................................................................................
238
14
DANKSAGUNG....................................................................................................................247
|
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| spelling | Ewe, Alexander 1984- Verfasser (DE-588)114065196X aut Development and analysis of improved Polyethylenimine (PEI)-based nanoparticles for the delivery of therapeutic nucleic acids in vitro and in vivo studies vorgelegt von Diplomchemiker Alexander Ewe Leipzig [2017] 1 CD-ROM (248 Blätter) Illustrationen, Diagramme txt rdacontent c rdamedia cd rdacarrier Dissertation Universität Leipzig 2017 (DE-588)4113937-9 Hochschulschrift gnd-content (DE-588)4139307-7 CD-ROM gnd-carrier DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030072205&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Textdatei PDF |
| spellingShingle | Ewe, Alexander 1984- Development and analysis of improved Polyethylenimine (PEI)-based nanoparticles for the delivery of therapeutic nucleic acids in vitro and in vivo studies |
| subject_GND | (DE-588)4113937-9 (DE-588)4139307-7 |
| title | Development and analysis of improved Polyethylenimine (PEI)-based nanoparticles for the delivery of therapeutic nucleic acids in vitro and in vivo studies |
| title_auth | Development and analysis of improved Polyethylenimine (PEI)-based nanoparticles for the delivery of therapeutic nucleic acids in vitro and in vivo studies |
| title_exact_search | Development and analysis of improved Polyethylenimine (PEI)-based nanoparticles for the delivery of therapeutic nucleic acids in vitro and in vivo studies |
| title_full | Development and analysis of improved Polyethylenimine (PEI)-based nanoparticles for the delivery of therapeutic nucleic acids in vitro and in vivo studies vorgelegt von Diplomchemiker Alexander Ewe |
| title_fullStr | Development and analysis of improved Polyethylenimine (PEI)-based nanoparticles for the delivery of therapeutic nucleic acids in vitro and in vivo studies vorgelegt von Diplomchemiker Alexander Ewe |
| title_full_unstemmed | Development and analysis of improved Polyethylenimine (PEI)-based nanoparticles for the delivery of therapeutic nucleic acids in vitro and in vivo studies vorgelegt von Diplomchemiker Alexander Ewe |
| title_short | Development and analysis of improved Polyethylenimine (PEI)-based nanoparticles for the delivery of therapeutic nucleic acids |
| title_sort | development and analysis of improved polyethylenimine pei based nanoparticles for the delivery of therapeutic nucleic acids in vitro and in vivo studies |
| title_sub | in vitro and in vivo studies |
| topic_facet | Hochschulschrift CD-ROM |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030072205&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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