Local and global analysis of the structure-function relation in chromatin:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
|
| Schlagworte: | |
| Online-Zugang: | kostenfrei https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-279296 Inhaltsverzeichnis |
| Beschreibung: | 336 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV041736410 | ||
| 003 | DE-604 | ||
| 005 | 20140610 | ||
| 007 | t | ||
| 008 | 140314s2013 ad|| m||| 00||| eng d | ||
| 035 | |a (OCoLC)878013602 | ||
| 035 | |a (DE-599)BVBBV041736410 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-384 |a DE-473 |a DE-703 |a DE-1051 |a DE-824 |a DE-29 |a DE-12 |a DE-91 |a DE-19 |a DE-1049 |a DE-92 |a DE-739 |a DE-898 |a DE-355 |a DE-706 |a DE-20 |a DE-1102 | ||
| 082 | 0 | |a 570 | |
| 084 | |a WE 4500 |0 (DE-625)148293: |2 rvk | ||
| 100 | 1 | |a Diermeier, Sarah Daniela Helena |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Local and global analysis of the structure-function relation in chromatin |c von Sarah Daniela Helena Diermeier |
| 264 | 1 | |c 2013 | |
| 300 | |a 336 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Regensburg, Univ., Diss., 2013 | ||
| 650 | 0 | 7 | |a Struktur-Aktivitäts-Beziehung |0 (DE-588)4183784-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Genregulation |0 (DE-588)4122166-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Ribosomale RNS |0 (DE-588)4076715-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Chromatin |0 (DE-588)4010152-6 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Chromatin |0 (DE-588)4010152-6 |D s |
| 689 | 0 | 1 | |a Struktur-Aktivitäts-Beziehung |0 (DE-588)4183784-8 |D s |
| 689 | 0 | 2 | |a Genregulation |0 (DE-588)4122166-7 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Ribosomale RNS |0 (DE-588)4076715-2 |D s |
| 689 | 1 | 1 | |a Chromatin |0 (DE-588)4010152-6 |D s |
| 689 | 1 | 2 | |a Struktur-Aktivitäts-Beziehung |0 (DE-588)4183784-8 |D s |
| 689 | 1 | |C b |5 DE-604 | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:355-epub-279296 |
| 856 | 4 | 1 | |u http://epub.uni-regensburg.de/27929/ |x Verlag |z kostenfrei |3 Volltext |
| 856 | 4 | |u https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-279296 |x Resolving-System | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027183099&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-027183099 | ||
Datensatz im Suchindex
| _version_ | 1804152022565388288 |
|---|---|
| adam_text | CONTENTS
LIST OF FIGURES 9
LIST OF TABLES 11
ABBREVIATIONS 13
1 SUMMARY 17
2 GENERAL INTRODUCTION 19
2.1 DISCOVERY OF DNA STRUCTURE AND FUNCTION - FROM MIESCHER TO ENCODE 19
2.2 CHROMATIN ARCHITECTURE: THE PRINCIPLES OF PACKAGING DNA 21
2.3 SPATIAL ORGANIZATION OF CHROMATIN IN THE NUCLEUS 24
2.4 CHROMATIN DYNAMICS 25
2.4.1 CHROMATIN MOTION 26
2.4.2 ATP-DEPENDENT CHROMATIN REMODELING 27
2.4.3 HISTONE CHAPERONES 28
2.4.4 NUCLEOTIDE VARIANTS 29
2.4.5 HISTONE VARIANTS 30
2.4.6 POST-TRANSLATIONAL HISTONE TAIL MODIFICATIONS 30
2.4.7 CHROMATIN ARCHITECTURAL PROTEINS (CAPS) 32
3 OBJECTIVES 33
3.1 GLOBAL ANALYSIS OF CHROMATIN STRUCTURE AND DYNAMICS OF NUCLEOSOME
POSITIONING 33
3.2 RIBOSOMAL DNA AS A MODEL LOCUS FOR CHROMATIN DYNAMICS 34
4 GLOBAL ANALYSIS OF CHROMATIN STRUCTURE AND DYNAMICS OF NUCLEOSOME
POSITIONING 35
4.1 INTRODUCTION 35
4.1.1 CHROMATIN STATES 35
4.1.2 CHROMATIN ACCESSIBILITY 37
4.1.3 NUCLEOSOME POSITIONING 39
4.2 RESULTS 42
4.2.1 CHANGES IN CHROMATIN ACCESSIBILITY UPON DIFFERENTIATION 42
4.2.1.1 ESTABLISHING THE FA1RE ASSAY IN PRIMARY HUMAN
CELLS 42
4.2.1.2 GENOME-WIDE CHANGES IN CHROMATIN ACCESSIBILITY 44
4.2.2 IDENTIFICATION AND CHARACTERIZATION OF OPEN AND CLOSED CHROMATIN
DOMAINS 46
4.2.2.1 ISOLATION AND SEPARATION OF CHROMATIN DOMAINS 46
4.2.2.2 SINGLE CELL ANALYSIS OF CHROMATIN ACCESSIBILITY 48
4.2.2.3 CHROMATIN ACCESSIBILITY IS LINKED TO GENE EXPRESSION 48
4.2.2.4 CONDENSED CHROMATIN STRUCTURES ANTI-CORRELATE WITH GENE DENSITY
AND
EXPRESSION 53
4.2.2.5 CHROMATIN STATES DISPLAY CHARACTERISTIC NUCLEOSOME SPACING 55
4.2.2.6 CHROMATIN ACCESSIBILITY CORRELATES WITH SPECIFIC REGULATORY
MARKS 57
4.2.3 TNFA INDUCES GENOME-WIDE NUCLEOSOME REPOSITIONING 59
4.2.3.1 GLOBAL CHANGES IN NUCLEOSOME POSITIONING UPON INFLAMMATION 59
4.2.3.2 THE CHROMATIN STRUCTURE AROUND AN INDUCIBLE TSS OPENS UP 61
4.2.3.3 DISTINCT HISTONE MODIFICATIONS MARK THE TSS OF RESPONSIVE GENES
62
-5-
HTTP://D-NB.INFO/1049972066
CONTENTS
4.2.3.4 NUCLEOSOME POSITIONING AND HISTONE MODIFICATIONS AROUND
RECURSIVE SPLICE
SITES 65
4.2.3.5 INDUCED GENES ARE OVERALL DEPLETED OF NUCLEOSOMES 69
4.2.3.6 GLOBAL NUCLEOSOME REPOSITIONING OCCURS AROUND TNFA-SPECIFIC
SEQUENCE
MOTIFS 71
4.2.3.7 TNFA RELATED CHANGES IN NUCLEOSOME POSITIONING ARE LINKED TO
PROMOTER
ELEMENTS AND ALU REPEATS 76
4.2.4 ALTERED CHROMATIN STRUCTURE IN TRISOMY 21 IS RELATED TO CHANGES IN
HISTONE
EXPRESSION 78
4.3 DISCUSSION 81
4.3.1 FUNCTIONAL ELEMENTS ARE DEPLETED OF NUCLEOSOMES IN A CELL
TYPE-SPECIFIC
MANNER 81
4.3.2 CHROMATIN ACCESSIBILITY IS CORRELATED WITH FUNCTION 82
4.3.3 STIMULATION WITH CYTOKINES INDUCE GENOME-WIDE, DIRECTED CHANGES IN
NUCLEOSOME POSITIONS 84
4.3.4 ALTERATIONS IN GLOBAL CHROMATIN STRUCTURE ARE LINKED TO HISTONE
EXPRESSION
LEVELS 88
RIBOSOMAL DNA AS A MODEL LOCUS FOR CHROMATIN DYNAMICS 91
5.1 INTRODUCTION 91
5.1.1 CHROMATIN LOOPING 91
5.1.2 RIBOSOMAL DNA 94
5.1.3 THE NUCLEOLUS 97
5.1.4 TRANSCRIPTION TERMINATION FACTOR I (TTF-I) 99
5.1.5 HTCS AND COOPERATIVE BINDING 101
5.2 RESULTS 103
5.2.1 MOUSE TTF-I OLIGOMERIZES C- AND N-TERMINALLY 103
5.2.2 ESTABLISHING AN RDNA SINGLE COPY MODEL SYSTEM 106
5.2.3 CLUSTERING OF RDNA TERMINATION SITES IS EVOLUTIONARY CONSERVED 107
5.2.4 TTF-I BINDS WITH SIMILAR AFFINITY TO ALL TERMINATION SITES IN
VITRO 109
5.2.5 CLUSTERED TERMINATION SITES FACILITATE COOPERATIVE BINDING OF
TTF-I TO
CHROMATIN 113
5.2.6 MULTIPLE TERMINATION SITES ENHANCE TRANSCRIPTION 113
5.2.7 CONSTRUCTION AND CHARACTERIZATION OF STABLE CELL LINES CONTAINING
A SINGLE
RDNA COPY 116
5.2.8 HTC IS REQUIRED FOR EFFICIENT LOADING OF RNAPI FACTORS 121
5.2.9 TTF-I BOUND TO CLUSTERED TERMINATION SITES FACILITATE
PROMOTER-TERMINATOR
CHROMATIN LOOPING 122
5.2.10 INTEGRATIVE ANALYSIS OF HISTONE MARKS REVEALS SIMIL- ARITY TO
CLASSICAL
ENHANCER ELEMENTS 124
5.3 DISCUSSION 126
5.3.1 MOUSE TTF-I OLIGOMERIZES C-AND N-TERMINALLY 126
5.3.2 CLUSTERED TERMINATION SITES FORM A CHROMATIN-SPECIFIC HIGH
AFFINITY BINDING
PLATFORM 127
5.3.3 MULTIPLE TTF-I BINDING SITES ENHANCE TRANSCRIPTION BY RECRUITING
RNAPI AND
ASSOCIATED FACTORS 129
-6-
CONTENTS
5.3.4 TTF-I AND CLUSTERED BINDING SITES ARE REQUIRED FOR CHROMATIN
LOOPING 131
5.3.5 THE MOUSE RDNA TERMINATOR ACTS AS AN ENHANCER 133
5.3.6 PERSPECTIVES 134
6 MATERIALS AND METHODS 137
6.1 MATERIALS 137
6.1.1 LABORATORY CHEMICALS AND REAGENTS 137
6.1.2 KITS AND READY-MADE SOLUTIONS 140
6.1.3 BUFFERS AND STANDARD SOLUTIONS 141
6.1.4 CONSUMABLES 145
6.1.5 CELL CULTURE REAGENTS 146
6.1.6 MAMMALIAN CELL LINES 147
6.1.7 ENZYMES 148
6.1.8 BACTERIAL STRAINS 148
6.1.9 ANTIBODIES 149
6.1.10 HIGH-THROUGHPUT DATASETS 149
6.1.11 SOFTWARE AND ONLINE TOOLS 152
6.1.12 OLIGONUCLEOTIDES 153
6.1.13 PLASMIDS 160
6.2 METHODS 165
6.2.1 MOLECULAR CLONING 165
6.2.1.1 ISOLATION OF PLASMID DNA .....165
6.2.1.2 RESTRICTION ENZYME DIGEST 165
6.2.1.3 MODIFYING COHESIVE ENDS TO BLUNT ENDS 166
6.2.1.4 DEPHOSPHORYLATION OF BLUNT ENDS 166
6.2.1.5 POLYMERASE CHAIN REACTION (PCR) 166
6.2.1.6 PURIFICATION OF PCR PRODUCTS 168
6.2.1.7 AGAROSE GEL EXTRACTION 168
6.2.1.8 LIGATION 168
6.2.1.9 TRANSFORMATION 169
6.2.1.10 COLONY PCR 169
6.2.1.11 BACTERIAL CRYO CULTURES 171
6.2.2 MAMMALIAN CELL CULTURE 171
6.2.2.1 MAINTENANCE AND PROPAGATION 171
6.2.2.2 MAMMALIAN CRYO CULTURES 172
6.2.2.3 TRANSIENT TRANSFECTION 173
6.2.2.4 GENERATION OF STABLE FLP-IN CELL LINES 173
6.2.3 WORKING WITH NUCLEIC ACIDS 174
6.2.3.1 DETERMINATION OF DNA QUANTITY AND PURITY 174
6.2.3.2 PHENOL/CHLOROFORM EXTRACTION 175
6.2.3.3 DNA PRECIPITATION 176
6.2.3.4 AGAROSE GEL ELECTROPHORESIS 176
6.2.3.5 ANNEALING OF OLIGONUCLEOTIDES 176
6.2.3.6 ELECTROMOBILITY SHIFT ASSAY (EMSA) 177
6.2.3.7 NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS (PAGE) 177
6.2.3.8 MICROSCALE THERMOPHORESIS (MST) 177
-7-
CONTENTS
6.2.3.9 RNA EXTRACTION 178
6.2.3.10 CDNA SYNTHESIS 178
6.2.3.11 QUANTITATIVE REAL-TIME PCR (QPCR) 179
6.2.4 WORKING WITH PROTEINS 180
6.2.4.1 PROTEIN EXTRACTION 180
6.2.4.2 QUANTITATION OF PROTEIN CONCENTRATION 181
6.2.4.3 DENATURING POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE) 181
6.2.4.4 BLUE NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS 182
6.2.4.5 COOMASSIE STAINING 182
6.2.4.6 WESTERN BLOT AND IMMUNO-DETECTION 182
6.2.4.7 DYNAMIC LIGHT SCATTERING 183
6.2.4.8 REPORTER GENE ASSAYS 184
6.2.5 WORKING WITH CHROMATIN 185
6.2.5.1 CHROMATIN IMMUNO-PRECIPITATION (CHIP) 185
6.2.5.2 FORMALDEHYDE-ASSISTED ISOLATION OF REGULATORY ELEMENTS (FA1RE)
187
6.2.5.3 PARTIAL MICROCOCCAL NUCLEASE (MNASE) DIGESTION 188
6.2.6 FLUORESCENCE IN SITU HYBRIDIZATION (FISH) 188
6.2.6.1 2D FISH 188
6.2.6.2 3D FISH 189
6.2.6.3 RNA FISH 190
6.2.7 MICROARRAYS 190
6.2.8 HIGH-THROUGHPUT SEQUENCING 191
6.2.9 BIOINFORMATIC ANALYSIS OF HIGH-THROUGHPUT DATASETS 191
7 REFERENCES 193
8 APPENDIX 219
8.1 ACCEPTED MANUSCRIPTS 219
8.1.1 FELLEETAL 219
8.1.2 SCHUBERT ET AL 231
8.2 SUBMITTED MANUSCRIPTS 243
8.2.1 DIERMEIER ET AL. (RDNA) 243
8.2.2 DIERMEIER ET AL. (METHODS CARNA I) 251
8.2.3 DIERMEIER ET AL. (METHODS CARNA II) 267
8.2.4 DIERMEIER ET AL. (METHODS MNASE-SEQ) 277
8.2.5 KRINNER ET AL 295
8.3 MANUSCRIPTS IN PREPARATION 333
ACKNOWLEDGEMENTS 335
-8-
|
| any_adam_object | 1 |
| author | Diermeier, Sarah Daniela Helena |
| author_facet | Diermeier, Sarah Daniela Helena |
| author_role | aut |
| author_sort | Diermeier, Sarah Daniela Helena |
| author_variant | s d h d sdh sdhd |
| building | Verbundindex |
| bvnumber | BV041736410 |
| classification_rvk | WE 4500 |
| collection | ebook |
| ctrlnum | (OCoLC)878013602 (DE-599)BVBBV041736410 |
| dewey-full | 570 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 570 - Biology |
| dewey-raw | 570 |
| dewey-search | 570 |
| dewey-sort | 3570 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02199nam a2200493 c 4500</leader><controlfield tag="001">BV041736410</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20140610 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">140314s2013 ad|| m||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)878013602</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV041736410</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-384</subfield><subfield code="a">DE-473</subfield><subfield code="a">DE-703</subfield><subfield code="a">DE-1051</subfield><subfield code="a">DE-824</subfield><subfield code="a">DE-29</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-91</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-1049</subfield><subfield code="a">DE-92</subfield><subfield code="a">DE-739</subfield><subfield code="a">DE-898</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-706</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-1102</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">570</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WE 4500</subfield><subfield code="0">(DE-625)148293:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Diermeier, Sarah Daniela Helena</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Local and global analysis of the structure-function relation in chromatin</subfield><subfield code="c">von Sarah Daniela Helena Diermeier</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2013</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">336 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Regensburg, Univ., Diss., 2013</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Struktur-Aktivitäts-Beziehung</subfield><subfield code="0">(DE-588)4183784-8</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Genregulation</subfield><subfield code="0">(DE-588)4122166-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Ribosomale RNS</subfield><subfield code="0">(DE-588)4076715-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Chromatin</subfield><subfield code="0">(DE-588)4010152-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Chromatin</subfield><subfield code="0">(DE-588)4010152-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Struktur-Aktivitäts-Beziehung</subfield><subfield code="0">(DE-588)4183784-8</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Genregulation</subfield><subfield code="0">(DE-588)4122166-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Ribosomale RNS</subfield><subfield code="0">(DE-588)4076715-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Chromatin</subfield><subfield code="0">(DE-588)4010152-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="2"><subfield code="a">Struktur-Aktivitäts-Beziehung</subfield><subfield code="0">(DE-588)4183784-8</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="C">b</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="o">urn:nbn:de:bvb:355-epub-279296</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">http://epub.uni-regensburg.de/27929/</subfield><subfield code="x">Verlag</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-279296</subfield><subfield code="x">Resolving-System</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027183099&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-027183099</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV041736410 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T01:04:06Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027183099 |
| oclc_num | 878013602 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| owner_facet | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| physical | 336 S. Ill., graph. Darst. |
| psigel | ebook |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| record_format | marc |
| spelling | Diermeier, Sarah Daniela Helena Verfasser aut Local and global analysis of the structure-function relation in chromatin von Sarah Daniela Helena Diermeier 2013 336 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Regensburg, Univ., Diss., 2013 Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 gnd rswk-swf Genregulation (DE-588)4122166-7 gnd rswk-swf Ribosomale RNS (DE-588)4076715-2 gnd rswk-swf Chromatin (DE-588)4010152-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Chromatin (DE-588)4010152-6 s Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 s Genregulation (DE-588)4122166-7 s DE-604 Ribosomale RNS (DE-588)4076715-2 s b DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:355-epub-279296 http://epub.uni-regensburg.de/27929/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-279296 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027183099&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Diermeier, Sarah Daniela Helena Local and global analysis of the structure-function relation in chromatin Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 gnd Genregulation (DE-588)4122166-7 gnd Ribosomale RNS (DE-588)4076715-2 gnd Chromatin (DE-588)4010152-6 gnd |
| subject_GND | (DE-588)4183784-8 (DE-588)4122166-7 (DE-588)4076715-2 (DE-588)4010152-6 (DE-588)4113937-9 |
| title | Local and global analysis of the structure-function relation in chromatin |
| title_auth | Local and global analysis of the structure-function relation in chromatin |
| title_exact_search | Local and global analysis of the structure-function relation in chromatin |
| title_full | Local and global analysis of the structure-function relation in chromatin von Sarah Daniela Helena Diermeier |
| title_fullStr | Local and global analysis of the structure-function relation in chromatin von Sarah Daniela Helena Diermeier |
| title_full_unstemmed | Local and global analysis of the structure-function relation in chromatin von Sarah Daniela Helena Diermeier |
| title_short | Local and global analysis of the structure-function relation in chromatin |
| title_sort | local and global analysis of the structure function relation in chromatin |
| topic | Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 gnd Genregulation (DE-588)4122166-7 gnd Ribosomale RNS (DE-588)4076715-2 gnd Chromatin (DE-588)4010152-6 gnd |
| topic_facet | Struktur-Aktivitäts-Beziehung Genregulation Ribosomale RNS Chromatin Hochschulschrift |
| url | http://epub.uni-regensburg.de/27929/ https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-279296 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027183099&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT diermeiersarahdanielahelena localandglobalanalysisofthestructurefunctionrelationinchromatin |