Conformational characterization of peptides derived from the neck region of fungal kinesin:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2001
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 116 S. graph. Darst. : 21 cm |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV014268826 | ||
| 003 | DE-604 | ||
| 005 | 20030709 | ||
| 007 | t | ||
| 008 | 020423s2001 gw d||| m||| 00||| eng d | ||
| 016 | 7 | |a 963260596 |2 DE-101 | |
| 035 | |a (OCoLC)51516058 | ||
| 035 | |a (DE-599)BVBBV014268826 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-29T |a DE-91 |a DE-12 |a DE-188 | ||
| 100 | 1 | |a Deluca, Dominga |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Conformational characterization of peptides derived from the neck region of fungal kinesin |c Dominga Deluca |
| 264 | 1 | |c 2001 | |
| 300 | |a 116 S. |b graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Techn. Univ., Diss. | ||
| 650 | 0 | 7 | |a Konformationsanalyse |0 (DE-588)4164967-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Kinesin |0 (DE-588)4236029-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Peptide |0 (DE-588)4045125-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Pilze |0 (DE-588)4046076-9 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Pilze |0 (DE-588)4046076-9 |D s |
| 689 | 0 | 1 | |a Kinesin |0 (DE-588)4236029-8 |D s |
| 689 | 0 | 2 | |a Peptide |0 (DE-588)4045125-2 |D s |
| 689 | 0 | 3 | |a Konformationsanalyse |0 (DE-588)4164967-9 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009785910&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009785910 | |
Datensatz im Suchindex
| _version_ | 1807504209333977088 |
|---|---|
| adam_text |
CONTENTS
1.
INTRODUCTION
1
1.1.1
KINESINS
AS
MOLECULAR
MOTORS
1
1.1.2
CONVENTIONAL
KINESINS
3
1.1.3
THE
MOTOR
DOMAINS
4
1.1.4
THE
NECK
REGION
6
1.1.5
CONVENTIONAL
KINESINS
AND
MICROTUBULE
INTERACTIONS
8
1.1.6
THE
MOVEMENT
OF
KINESINS
ALONG
THE
MICROTUBULES
10
1.1.7
FUNGAL
KINESINS
12
1.1.8
THE
KINESIN
SUPERFAMILY
14
1.2
COILED-COIL
STRUCTURES
15
1.2.1
TWO-STRANDED
CT-HELICAL
COILED-COILS
15
1.2.2
SPATIAL
DISPLAY
OF
THE
TWO
HELICES
IN
A-HELICAL
COILED-COILS
17
1.2.3
HYDROPHOBIC
INTERACTIONS
IN
A-HELICAL
COILED
COILS
17
1.2.4
HYDROPHILIC
INTERACTIONS
IN
A-HELICAL
COILED
COILS
19
1.2.5
EFFECT
OF
PH
ON
THE
STABILITY
OF
A-HELICAL
COILED-COILS
21
1.2.6
STABILISATION
OF
A-HELICAL
COILED-COILS
BY
DISULFIDE
BRIDGES
22
1.2.7
OLIGOMERISATION
STATE
OF
A-HELICAL
COILED-COILS
24
2.
OBJECTIVES
25
3.
RESULTS
27
3.1
THEORETICAL
ASPECTS
FOR
THE
SELECTION
OF
PEPTIDE
FRAGMENTS
OF
THE
KINESIN
NECK
REGION
FOR
CONFORMATIONAL
STUDIES
27
3.2
PEPTIDE
SYNTHESIS
33
3.2.1
SYNTHESIS
OF
MONOMERS
KNL
AND
KN2
33
3.2.2
SYNTHESIS
OF
THE
PEPTIDE
DIMERS
KN3,
KN4,
KN5
AND
KN6
36
3.3
DICHROIC
PROPERTIES
OF
A-HELICAL
COILED-COILS
42
3.3.1
CONFORMATIONAL
ANALYSIS
OF
THE
MONOMERIC
PEPTIDES
KNL
AND
KN2
43
3.3.1.1
EFFECT
OF
PH
ON
THE
CONFORMATIONAL
STATES
OF
THE
MONOMERIC
KNL
AND
KN2
PEPTIDES
43
3.3.1.2
EFFECT
OF
TRIFLUOROETHANOL
ON
THE
CONFORMATIONAL
STATES
OF
THE
MONOMERIC
KNL
AND
KN2
PEPTIDES
46
3.3.1.3
EFFECT
OF
PEPTIDE
CONCENTRATION
ON
THE
CONFORMATIONAL
STATES
OF
THE
MONOMERIC
KNL
AND
KN2
PEPTIDES
48
3.3.1.4
THERMAL
DENATURATION
OF
THE
MONOMERIC
KNL
AND
KN2
PEPTIDES
50
3.3.1.5
MASS
SPECTRA
OF
THE
MONOMERIC
KNL
AND
KN2
PEPTIDES
53
3.3.2
CONFORMATIONAL
ANALYSIS
OF
THE
HOMODIMERIC
KN3-KN6
PEPTIDES
56
3.3.2.1
EFFECT
OF
PH
ON
THE
CONFORMATIONAL
STATES
OF
THE
HOMODIMERIC
KN3-KN6
PEPTIDES
56
3.3.2.2
THERMAL
DENATURATION
OF
THE
HOMODIMERIC
KN3-KN6
PEPTIDES
62
4.
DISCUSSION
69
5.
SUMMARY
AND
PERSPECTIVES
72
6.
EXPERIMENTAL
PART
75
6.1
MATERIALS
AND
METHODS
75
6.2
PEPTIDE
SYNTHESIS
86
6.2.1
SYNTHESIS
THROUGH
FMOC/TBU
CHEMISTRY
AT
THE
AUTOMATED
SYNTHESISER
86
6.2.2
SYNTHESIS
THROUGH
FMOC/TBU
CHEMISTRY
WITH
MANUAL
PROCEDURE
87
6.2.3
CLEAVAGE
OF
THE
PEPTIDE
FROM
THE
RESIN
WITH
TFA
87
6.2.4
SYNTHESIS
WITH
BOC/BZL
CHEMISTRY
WITH
MANUAL
PROCEDURE
88
6.2.5
CLEAVAGE
OF
THE
PEPTIDE
FROM
THE
RESIN
WITH
HF
91
6.2.6
ACETYLATION
91
6.2.7
KAISER
TEST
91
6.2.8
S-TBU
CLEAVAGE
WITH
TRI-N-BUTYL-PHOSPHINE
92
6.2.9
DISULFIDE
BOND
FORMATION
THROUGH
OXIDATION
WITH
DMSO
IN
TFA
92
6.2.10
DISULFIDE
BOND
FORMATION
THROUGH
OXIDATION
WITH
DMSO
IN
AQUEOUS
BUFFER
93
6.2.11
DISULFIDE
BRIDGE
FORMATION
THROUGH
AIR-OXIDATION
93
6.2.12
DETERMINATION
OF
SULFHYDRYL
GROUPS
WITH
2,2
'
-DITHIODIPYRIDINE
(GRASSETTI
TEST)
94
6.3
SYNTHESIS
OF
THE
SINGLE
PEPTIDES
96
7.
REFERENCES
106 |
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| author | Deluca, Dominga |
| author_facet | Deluca, Dominga |
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| author_sort | Deluca, Dominga |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV014268826 |
| illustrated | Illustrated |
| indexdate | 2024-08-16T01:05:38Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009785910 |
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| owner_facet | DE-29T DE-91 DE-BY-TUM DE-12 DE-188 |
| physical | 116 S. graph. Darst. : 21 cm |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| record_format | marc |
| spelling | Deluca, Dominga Verfasser aut Conformational characterization of peptides derived from the neck region of fungal kinesin Dominga Deluca 2001 116 S. graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss. Konformationsanalyse (DE-588)4164967-9 gnd rswk-swf Kinesin (DE-588)4236029-8 gnd rswk-swf Peptide (DE-588)4045125-2 gnd rswk-swf Pilze (DE-588)4046076-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Pilze (DE-588)4046076-9 s Kinesin (DE-588)4236029-8 s Peptide (DE-588)4045125-2 s Konformationsanalyse (DE-588)4164967-9 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009785910&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Deluca, Dominga Conformational characterization of peptides derived from the neck region of fungal kinesin Konformationsanalyse (DE-588)4164967-9 gnd Kinesin (DE-588)4236029-8 gnd Peptide (DE-588)4045125-2 gnd Pilze (DE-588)4046076-9 gnd |
| subject_GND | (DE-588)4164967-9 (DE-588)4236029-8 (DE-588)4045125-2 (DE-588)4046076-9 (DE-588)4113937-9 |
| title | Conformational characterization of peptides derived from the neck region of fungal kinesin |
| title_auth | Conformational characterization of peptides derived from the neck region of fungal kinesin |
| title_exact_search | Conformational characterization of peptides derived from the neck region of fungal kinesin |
| title_full | Conformational characterization of peptides derived from the neck region of fungal kinesin Dominga Deluca |
| title_fullStr | Conformational characterization of peptides derived from the neck region of fungal kinesin Dominga Deluca |
| title_full_unstemmed | Conformational characterization of peptides derived from the neck region of fungal kinesin Dominga Deluca |
| title_short | Conformational characterization of peptides derived from the neck region of fungal kinesin |
| title_sort | conformational characterization of peptides derived from the neck region of fungal kinesin |
| topic | Konformationsanalyse (DE-588)4164967-9 gnd Kinesin (DE-588)4236029-8 gnd Peptide (DE-588)4045125-2 gnd Pilze (DE-588)4046076-9 gnd |
| topic_facet | Konformationsanalyse Kinesin Peptide Pilze Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009785910&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT delucadominga conformationalcharacterizationofpeptidesderivedfromtheneckregionoffungalkinesin |