Antigen-independent signalling of the antigen receptor in chronic lymphocytic leukaemia: pathophysiological relevance
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Enth. außerdem 2 Sonderabdr. aus: Nature. 2012 und: Blood ; Vol. 116. 2010 |
| Beschreibung: | 97, [8] Bl. Ill., graph. Darst. 30 cm |
Internformat
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Datensatz im Suchindex
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| adam_text | IMAGE 1
1. TABLE O F CONTENT
1. TABLE OF CONTENT 5
1.1 TABLE OF FIGURES 8
2. SUMMARY 10
3. INTRODUCTION 13
3.1. THE IMMUNE SYSTEM 13
3.2. THE B CELL DEVELOPMENT 15
3.3 THE B CELL ANTIGEN RECEPTOR COMPLEX 19
3.4 BCR SIGNALLING 21
3.5. B CELL LYMPHOMAS 24
3.5.1. CHRONIC LYMPHOCYTIC LEUKAEMIA 25
3.5.2. CLL DIAGNOSIS AND STAGING 25
3.5.3. PROGNOSIS AND MOLECULAR PATHOGENESIS OF CLL 26
3.5.4 THE BCR IN CLL 28
4. AIM OF THE STUDY 30
5. RESULTS 31
5.1. CLL-DERIVED ANTIGEN RECEPTORS INDUCE AUTONOMOUS SIGNALING 31
5.2. PRIMARY HUMAN CLL LYMPHOCYTES SHOW ELEVATED CA 2+ LEVELS 33
5.3. THE HCDR3 DOMAIN IS CRUCIAL FOR MEDIATING ANTIGEN-INDEPENDENT
SIGNALLING
OF CLL-DERIVED BCRS 34
5.4. AUTONOMOUS BCR SIGNALLING CAPACITY OF CLL-DERIVED BCRS IS NOT
ASSOCIATED WITH POLYREACTIVITY 36
5.5. CLL-DERIVED BCRS INDUCE AUTONOMOUS SIGNALLING IN A CELL AUTONOMOUS
AND
EXTERNAL ANTIGEN-INDEPENDENT MANNER 38
5.7. EXPRESSION AS IGD DOES NOT INDUCE AUTONOMOUS SIGNALLING 41
5.8. E I-TCL1TG MICE DERIVED LYMPHOMA BCRS BEHAVE LIKE THEIR HUMAN CLL
COUNTERPARTS 42
5.9. THE ROLE OF THE BCR-CLASS IN DRIVING CLL PROLIFERATION IN VIVO 44
5.10. IGM TCL? MICE SHOW A ACCUMULATION OF CD19 + CD5 + CELLS 46
5
HTTP://D-NB.INFO/1032544414
IMAGE 2
5.11. DIFFERENT SURFACE EXPRESSION LEVELS OF IGM AND IGD IN THE TWO
MAJOR
SUBGROUPS OF CLL 47
6. DISCUSSION 50
7. MATERIAL AND METHODS 56
7.1. BLOOD SAMPLE ACQUISITION AND CLINICAL DATA 56
7.2. MOUSE STRAINS 56
7.3. MATERIAL 56
7.3.1. GENERAL LAB EQUIPMENT 56
7.3.2. CELL CULTURE EQUIPMENT 58
7.3.3. CHEMICALS AND REAGENTS 58
7.3.4. MEDIA AND SOLUTIONS 59
7.3.5. KITS 60
7.3.6. ENZYMES 60
7.3.7 VECTORS 60
7.3.8 OLIGONUCLEOTIDES 61
7.3.9. ANTIBODIES FOR FACS 61
7.3.10. CELL LINES 62
7.3.11. BACTERIAL STRAINS 62
7.3.13.SOFTWARE 62
7.4. METHODS 63
7.4.1. CELL CULTURE CONDITIONS 63
7.4.2. CLONING OF LYMPHOMA-DERIVED BCRS 63
7.4.3. HCDR3 TRANSFER 64
7.4.4. RETROVIRAL TRANSDUCTION OF TKO CELLS 64
7.4.5. FACS ANALYSIS 65
7.4.6. CA 2+ -FLUX MEASUREMENT OF TKO AND PRIMARY CELLS 65
7.4.7. SINGLE CELL CA 2+ -FLUX 65
7.4.8. ISOLATION OF SPLENOCYTES DERIVED FROM TCL1 MICE 66
8. ABBREVIATIONS 67
9. REFERENCES 70
10. APPENDIX 81
6
IMAGE 3
10.1. CLONING STRATEGY FOR LYMPHOMA DERIVED BCRS 81
10.2. BIFC VECTORS 82
10.4. LIST OF OLIGONUCLEOTIDES 84
10.4.1. PRIMER USED FOR CLONING LYMPHOMA BCRS 84
10.4.2. PRIMERS USED FOR HCDR3 TRANSFER 85
10.4.3. PRIMERS USED FOR SEQUENCING 85
10.5. SUPPLEMENTARY DATA 86
11. PUBLICATIONS 96
12. ACKNOWLEDGMENTS 97
7
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| spelling | Dühren- von Minden, Marcus Verfasser aut Antigen-independent signalling of the antigen receptor in chronic lymphocytic leukaemia pathophysiological relevance vorgelegt von Marcus Dühren-von Minden 2012 97, [8] Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Enth. außerdem 2 Sonderabdr. aus: Nature. 2012 und: Blood ; Vol. 116. 2010 Freiburg (Breisgau), Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026822906&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dühren- von Minden, Marcus Antigen-independent signalling of the antigen receptor in chronic lymphocytic leukaemia pathophysiological relevance |
| subject_GND | (DE-588)4113937-9 |
| title | Antigen-independent signalling of the antigen receptor in chronic lymphocytic leukaemia pathophysiological relevance |
| title_auth | Antigen-independent signalling of the antigen receptor in chronic lymphocytic leukaemia pathophysiological relevance |
| title_exact_search | Antigen-independent signalling of the antigen receptor in chronic lymphocytic leukaemia pathophysiological relevance |
| title_full | Antigen-independent signalling of the antigen receptor in chronic lymphocytic leukaemia pathophysiological relevance vorgelegt von Marcus Dühren-von Minden |
| title_fullStr | Antigen-independent signalling of the antigen receptor in chronic lymphocytic leukaemia pathophysiological relevance vorgelegt von Marcus Dühren-von Minden |
| title_full_unstemmed | Antigen-independent signalling of the antigen receptor in chronic lymphocytic leukaemia pathophysiological relevance vorgelegt von Marcus Dühren-von Minden |
| title_short | Antigen-independent signalling of the antigen receptor in chronic lymphocytic leukaemia |
| title_sort | antigen independent signalling of the antigen receptor in chronic lymphocytic leukaemia pathophysiological relevance |
| title_sub | pathophysiological relevance |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026822906&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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