Optical imaging techniques in cell biology:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Boca Raton, Fla. [u.a.]
CRC Press
2007
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Literaturangaben |
| Beschreibung: | 268 S. zahlr. Ill. und graph. Darst. |
| ISBN: | 0849339197 9780849339196 |
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| 245 | 1 | 0 | |a Optical imaging techniques in cell biology |c Guy C. Cox |
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OPTICAL IMAGING TECHNIQUES IN CELL BIOLOGY GUY COX SITSTFR' UIITL
L'DRTEI&S- BIBLIOTHAK &I IRIV.-NR. TAYLOR &. FRANCIS TAYLOR & FRANCIS
GROUP BOCA RATON LONDON NEW YORK CRC IS AN IMPRINT OF THE TAYLOR &
FRANCIS CROUP, AN INFORMA BUSINESS TABLE OF CONTENTS CHAPTER 1 THE LIGHT
MICROSCOPE 1 LENSES AND MICROSCOPES 1 THE BACK FOCAL PLANE OF A LENS 4
GOOD RESOLUTION 8 RESOLUTION: RAYLEIGH'S APPROACH 8 DIFFRACTION: ABBE'S
APPROACH 11 COHERENT AND INCOHERENT 14 ADD A DROP OF OIL 15 KOHLER
ILLUMINATION 17 CHAPTER 2 OPTICAL CONTRASTING TECHNIQUES 19 DARKFIELD 19
PHASE CONTRAST 21 POLARIZATION 22 DIFFERENTIAL INTERFERENCE CONTRAST 24
WHICH TECHNIQUE IS BEST? 27 CHAPTER 3 FLUORESCENCE AND FLUORESCENCE
MICROSCOPY 29 WHAT IS FLUORESCENCE? 29 WHAT MAKES A MOLECULE
FLUORESCENT? 33 THE FLUORESCENCE MICROSCOPE 34 OPTICAL ARRANGEMENT 34
LIGHT SOURCE 35 FILTER SETS 37 CHAPTER 4 IMAGE CAPTURE 43 OPTICAL LAYOUT
FOR IMAGE CAPTURE 43 FILM 43 MONOCHROME 44 COLOR FILMS 45 ADDITIVE COLOR
MODEL 45 SUBTRACTIVE COLOR MODEL 46 CCD CAMERAS 50 FRAME-TRANSFER ARRAY
52 INTERLINE-TRANSFER ARRAY 52 BACK ILLUMINATION 53 BINNING 53 RECORDING
COLOR 53 FILTER WHEELS 54 FILTER MOSAICS 55 THREE CCD ELEMENTS WITH
DICHROIC BEAMSPLITTERS 55 CHAPTER 5 THE CONFOCAL MICROSCOPE 57 THE
SCANNING OPTICAL MICROSCOPE 57 THE CONFOCAL PRINCIPLE 57 RESOLUTION AND
POINT SPREAD FUNCTION 60 LATERAL RESOLUTION IN THE CONFOCAL MICROSCOPE
62 PRACTICAL CONFOCAL MICROSCOPES 64 THE LIGHT SOURCE: LASERS 65 GAS
LASERS 66 SOLID-STATE LASERS 66 SEMICONDUCTOR LASERS 67 LASER DELIVERY
68 THE PRIMARY BEAMSPLITTER 69 BEAM SCANNING 70 PINHOLE AND SIGNAL
CHANNEL CONFIGURATIONS 72 DETECTORS 74 CHAPTER 6 THE DIGITAL IMAGE 77
PIXELS AND VOXELS 77 CONTRAST 77 SPATIAL SAMPLING: THE NYQUIST CRITERION
79 TEMPORAL SAMPLING: SIGNAL-TO-NOISE RATIO 81 MULTICHANNEL IMAGES 83
CHAPTER 7 ABERRATIONS AND THEIR CONSEQUENCES 87 GEOMETRICAL ABERRATIONS
87 SPHERICAL ABERRATION (SA) 87 COMA 92 ASTIGMATISM 93 FIELD CURVATURE
93 CHROMATIC ABERRATION 94 CHROMATIC DIFFERENCE OF MAGNIFICATION 97
PRACTICAL CONSEQUENCES 98 APPARENT DEPTH 99 CHAPTER 8 NONLINEAR
MICROSCOPY 101 MULTIPHOTON MICROSCOPY 101 PRINCIPLES OF TWO-PHOTON
FLUORESCENCE 101 THEORY AND PRACTICE 103 LASERS FOR NONLINEAR MICROSCOPY
104 ADVANTAGES OF TWO-PHOTON EXCITATION 106 CONSTRUCTION OF A
MULTIPHOTON MICROSCOPE 107 FLUOROCHROMES FOR MULTIPHOTON MICROSCOPY 108
SECOND HARMONIC MICROSCOPY 110 SUMMARY 113 CHAPTER 9 HIGH-SPEED CONFOCAL
MICROSCOPY 115 TANDEM-SCANNING (SPINNING DISK) MICROSCOPES 115 PETRAN
SYSTEM 115 ONE-SIDED TANDEM SCANNING MICROSCOPES (OTSMS) 118 MICROLENS
ARRAY: THE YOKOGAWA SYSTEM 119 SLIT-SCANNING MICROSCOPES 120 SLIT-SLIT
SYSTEMS 121 SPOT-SLIT SYSTEMS 122 MULTIPOINT-ARRAY SCANNERS 124
STRUCTURED ILLUMINATION 125 CHAPTER 10 DECONVOLUTION AND IMAGE
PROCESSING 127 DECONVOLUTION 127 DECONVOLVING CONFOCAL IMAGES 132 IMAGE
PROCESSING 133 GRAY-SCALE OPERATIONS 133 IMAGE ARITHMETIC 135
CONVOLUTION: SMOOTHING AND SHARPENING 136 CHAPTER 11 THREE-DIMENSIONAL
IMAGING: STEREOSCOPY AND RECONSTRUCTION 141 SURFACES: TWO-AND-A-HALF
DIMENSIONS 141 PERCEPTION OF THE 3D WORLD 142 LIMITATIONS OF CONFOCAL
MICROSCOPY 144 STEREOSCOPY 144 THREE-DIMENSIONAL RECONSTRUCTION 147
TECHNIQUES THAT REQUIRE IDENTIFICATION OF "OBJECTS" 147 TECHNIQUES THAT
CREATE VIEWS DIRECTLY FROM INTENSITY DATA 151 SIMPLE PROJECTIONS 151
WEIGHTED PROJECTION (ALPHA BLENDING) 153 CHAPTER 12 GREEN FLUORESCENT
PROTEIN 159 STRUCTURE AND PROPERTIES OF GFP 159 GFP VARIANTS 160
APPLICATIONS OF GFP 165 HEAT SHOCK 165 CATIONIC LIPID REAGENTS 165
DEAE-DEXTRAN AND POLYBRENE 165 CALCIUM PHOSPHATE COPRECIPITATION 166
ELECTROPORATION 166 MICROINJECTION 166 GENE GUN 166 PLANTS:
AGROBACTERIUM 167 CHAPTER 13 FLUORESCENT STAINING 169 IMMUNOLABELING 169
TYPES OF ANTIBODY 170 RAISING ANTIBODIES 171 LABELING 173 FLUORESCENT
STAINS FOR CELL COMPONENTS AND COMPARTMENTS 175 CHAPTER 14 QUANTITATIVE
FLUORESCENCE 181 FLUORESCENCE INTENSITY MEASUREMENTS 181 LINEARITY
CALIBRATION 181 MEASUREMENT 182 COLOCALIZATION 182 RATIO IMAGING 185
CELL LOADING 189 MEMBRANE POTENTIAL 189 SLOW-RESPONSE DYES 190
FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING 192 CHAPTER 15 ADVANCED
FLUORESCENCE TECHNIQUES: FLIM AND FRET 195 FLUORESCENCE LIFETIME 195
PRACTICAL LIFETIME MICROSCOPY 195 FREQUENCY DOMAIN 197 TIME DOMAIN 197
FLUORESCENCE RESONANT ENERGY TRANSFER (FRET) 200 WHY USE FRET? 201
IDENTIFYING AND QUANTIFYING FRET 204 INCREASE IN BRIGHTNESS OF ACCEPTOR
EMISSION 204 QUENCHING OF EMISSION FROM THE DONOR 204 LIFETIME OF DONOR
EMISSION 205 PROTECTION FROM BLEACHING OF DONOR 209 FLUORESCENCE
CORRELATION SPECTROSCOPY (FCS) 209 CHAPTER 16 BREAKING THE DIFFRACTION
LIMIT 213 NEAR-FIELD MICROSCOPY 213 TOTAL INTERNAL REFLECTION MICROSCOPY
217 4PI AND MULTIPLE-OBJECTIVE MICROSCOPY 220 STIMULATED EMISSION
DEPLETION (STED) 223 APPENDIX 1 MICROSCOPE CARE AND MAINTENANCE 227
CLEANING 229 THE FLUORESCENT ILLUMINATOR 231 APPENDIX 2 KEEPING CELLS
ALIVE UNDER THE MICROSCOPE 233 CHAMBERS 233 LIGHT 236 MOVEMENT 237
FINALLY 237 APPENDIX 3 ANTIBODY LABELING OF PLANT AND ANIMAL CELLS: TIPS
AND SAMPLE SCHEDULES 239 ANTIBODIES: TIPS ON HANDLING AND STORAGE 239
PIPETTES: TIPS ON HANDLING 240 ANTIBODIES AND ANTIBODY TITRATIONS 240
EXAMPLE 240 PRIMARY ANTIBODY TITRATION 242 IMMUNOFLUORESCENCE PROTOCOL
243 METHOD 243 MULTIPLE LABELING AND DIFFERENT SAMPLES 244 PLANT
MATERIAL 244 PROTOCOL 244 DIAGRAM SHOWING POSITION OF ANTIBODIES ON
MULTIWELL SLIDE 246 APPENDIX 4 IMAGE PROCESSING WITH IMAGEJ 249
INTRODUCTION 249 DIFFERENT WINDOWS IN IMAGEJ 250 IMAGE LEVELS 250 COLORS
AND LOOK-UP TABLES 250 SIZE CALIBRATION 252 IMAGE MATH 252
QUANTIFICATION 254 STACKS AND 3D REPRESENTATION 254 FFT AND IMAGE
PROCESSING 256 MACRO LANGUAGE IN IMAGEJ 257 INDEX 259 |
| adam_txt |
OPTICAL IMAGING TECHNIQUES IN CELL BIOLOGY GUY COX SITSTFR' UIITL
L'DRTEI&S- BIBLIOTHAK &I IRIV.-NR. TAYLOR &. FRANCIS TAYLOR & FRANCIS
GROUP BOCA RATON LONDON NEW YORK CRC IS AN IMPRINT OF THE TAYLOR &
FRANCIS CROUP, AN INFORMA BUSINESS TABLE OF CONTENTS CHAPTER 1 THE LIGHT
MICROSCOPE 1 LENSES AND MICROSCOPES 1 THE BACK FOCAL PLANE OF A LENS 4
GOOD RESOLUTION 8 RESOLUTION: RAYLEIGH'S APPROACH 8 DIFFRACTION: ABBE'S
APPROACH 11 COHERENT AND INCOHERENT 14 ADD A DROP OF OIL 15 KOHLER
ILLUMINATION 17 CHAPTER 2 OPTICAL CONTRASTING TECHNIQUES 19 DARKFIELD 19
PHASE CONTRAST 21 POLARIZATION 22 DIFFERENTIAL INTERFERENCE CONTRAST 24
WHICH TECHNIQUE IS BEST? 27 CHAPTER 3 FLUORESCENCE AND FLUORESCENCE
MICROSCOPY 29 WHAT IS FLUORESCENCE? 29 WHAT MAKES A MOLECULE
FLUORESCENT? 33 THE FLUORESCENCE MICROSCOPE 34 OPTICAL ARRANGEMENT 34
LIGHT SOURCE 35 FILTER SETS 37 CHAPTER 4 IMAGE CAPTURE 43 OPTICAL LAYOUT
FOR IMAGE CAPTURE 43 FILM 43 MONOCHROME 44 COLOR FILMS 45 ADDITIVE COLOR
MODEL 45 SUBTRACTIVE COLOR MODEL 46 CCD CAMERAS 50 FRAME-TRANSFER ARRAY
52 INTERLINE-TRANSFER ARRAY 52 BACK ILLUMINATION 53 BINNING 53 RECORDING
COLOR 53 FILTER WHEELS 54 FILTER MOSAICS 55 THREE CCD ELEMENTS WITH
DICHROIC BEAMSPLITTERS 55 CHAPTER 5 THE CONFOCAL MICROSCOPE 57 THE
SCANNING OPTICAL MICROSCOPE 57 THE CONFOCAL PRINCIPLE 57 RESOLUTION AND
POINT SPREAD FUNCTION 60 LATERAL RESOLUTION IN THE CONFOCAL MICROSCOPE
62 PRACTICAL CONFOCAL MICROSCOPES 64 THE LIGHT SOURCE: LASERS 65 GAS
LASERS 66 SOLID-STATE LASERS 66 SEMICONDUCTOR LASERS 67 LASER DELIVERY
68 THE PRIMARY BEAMSPLITTER 69 BEAM SCANNING 70 PINHOLE AND SIGNAL
CHANNEL CONFIGURATIONS 72 DETECTORS 74 CHAPTER 6 THE DIGITAL IMAGE 77
PIXELS AND VOXELS 77 CONTRAST 77 SPATIAL SAMPLING: THE NYQUIST CRITERION
79 TEMPORAL SAMPLING: SIGNAL-TO-NOISE RATIO 81 MULTICHANNEL IMAGES 83
CHAPTER 7 ABERRATIONS AND THEIR CONSEQUENCES 87 GEOMETRICAL ABERRATIONS
87 SPHERICAL ABERRATION (SA) 87 COMA 92 ASTIGMATISM 93 FIELD CURVATURE
93 CHROMATIC ABERRATION 94 CHROMATIC DIFFERENCE OF MAGNIFICATION 97
PRACTICAL CONSEQUENCES 98 APPARENT DEPTH 99 CHAPTER 8 NONLINEAR
MICROSCOPY 101 MULTIPHOTON MICROSCOPY 101 PRINCIPLES OF TWO-PHOTON
FLUORESCENCE 101 THEORY AND PRACTICE 103 LASERS FOR NONLINEAR MICROSCOPY
104 ADVANTAGES OF TWO-PHOTON EXCITATION 106 CONSTRUCTION OF A
MULTIPHOTON MICROSCOPE 107 FLUOROCHROMES FOR MULTIPHOTON MICROSCOPY 108
SECOND HARMONIC MICROSCOPY 110 SUMMARY 113 CHAPTER 9 HIGH-SPEED CONFOCAL
MICROSCOPY 115 TANDEM-SCANNING (SPINNING DISK) MICROSCOPES 115 PETRAN
SYSTEM 115 ONE-SIDED TANDEM SCANNING MICROSCOPES (OTSMS) 118 MICROLENS
ARRAY: THE YOKOGAWA SYSTEM 119 SLIT-SCANNING MICROSCOPES 120 SLIT-SLIT
SYSTEMS 121 SPOT-SLIT SYSTEMS 122 MULTIPOINT-ARRAY SCANNERS 124
STRUCTURED ILLUMINATION 125 CHAPTER 10 DECONVOLUTION AND IMAGE
PROCESSING 127 DECONVOLUTION 127 DECONVOLVING CONFOCAL IMAGES 132 IMAGE
PROCESSING 133 GRAY-SCALE OPERATIONS 133 IMAGE ARITHMETIC 135
CONVOLUTION: SMOOTHING AND SHARPENING 136 CHAPTER 11 THREE-DIMENSIONAL
IMAGING: STEREOSCOPY AND RECONSTRUCTION 141 SURFACES: TWO-AND-A-HALF
DIMENSIONS 141 PERCEPTION OF THE 3D WORLD 142 LIMITATIONS OF CONFOCAL
MICROSCOPY 144 STEREOSCOPY 144 THREE-DIMENSIONAL RECONSTRUCTION 147
TECHNIQUES THAT REQUIRE IDENTIFICATION OF "OBJECTS" 147 TECHNIQUES THAT
CREATE VIEWS DIRECTLY FROM INTENSITY DATA 151 SIMPLE PROJECTIONS 151
WEIGHTED PROJECTION (ALPHA BLENDING) 153 CHAPTER 12 GREEN FLUORESCENT
PROTEIN 159 STRUCTURE AND PROPERTIES OF GFP 159 GFP VARIANTS 160
APPLICATIONS OF GFP 165 HEAT SHOCK 165 CATIONIC LIPID REAGENTS 165
DEAE-DEXTRAN AND POLYBRENE 165 CALCIUM PHOSPHATE COPRECIPITATION 166
ELECTROPORATION 166 MICROINJECTION 166 GENE GUN 166 PLANTS:
AGROBACTERIUM 167 CHAPTER 13 FLUORESCENT STAINING 169 IMMUNOLABELING 169
TYPES OF ANTIBODY 170 RAISING ANTIBODIES 171 LABELING 173 FLUORESCENT
STAINS FOR CELL COMPONENTS AND COMPARTMENTS 175 CHAPTER 14 QUANTITATIVE
FLUORESCENCE 181 FLUORESCENCE INTENSITY MEASUREMENTS 181 LINEARITY
CALIBRATION 181 MEASUREMENT 182 COLOCALIZATION 182 RATIO IMAGING 185
CELL LOADING 189 MEMBRANE POTENTIAL 189 SLOW-RESPONSE DYES 190
FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING 192 CHAPTER 15 ADVANCED
FLUORESCENCE TECHNIQUES: FLIM AND FRET 195 FLUORESCENCE LIFETIME 195
PRACTICAL LIFETIME MICROSCOPY 195 FREQUENCY DOMAIN 197 TIME DOMAIN 197
FLUORESCENCE RESONANT ENERGY TRANSFER (FRET) 200 WHY USE FRET? 201
IDENTIFYING AND QUANTIFYING FRET 204 INCREASE IN BRIGHTNESS OF ACCEPTOR
EMISSION 204 QUENCHING OF EMISSION FROM THE DONOR 204 LIFETIME OF DONOR
EMISSION 205 PROTECTION FROM BLEACHING OF DONOR 209 FLUORESCENCE
CORRELATION SPECTROSCOPY (FCS) 209 CHAPTER 16 BREAKING THE DIFFRACTION
LIMIT 213 NEAR-FIELD MICROSCOPY 213 TOTAL INTERNAL REFLECTION MICROSCOPY
217 4PI AND MULTIPLE-OBJECTIVE MICROSCOPY 220 STIMULATED EMISSION
DEPLETION (STED) 223 APPENDIX 1 MICROSCOPE CARE AND MAINTENANCE 227
CLEANING 229 THE FLUORESCENT ILLUMINATOR 231 APPENDIX 2 KEEPING CELLS
ALIVE UNDER THE MICROSCOPE 233 CHAMBERS 233 LIGHT 236 MOVEMENT 237
FINALLY 237 APPENDIX 3 ANTIBODY LABELING OF PLANT AND ANIMAL CELLS: TIPS
AND SAMPLE SCHEDULES 239 ANTIBODIES: TIPS ON HANDLING AND STORAGE 239
PIPETTES: TIPS ON HANDLING 240 ANTIBODIES AND ANTIBODY TITRATIONS 240
EXAMPLE 240 PRIMARY ANTIBODY TITRATION 242 IMMUNOFLUORESCENCE PROTOCOL
243 METHOD 243 MULTIPLE LABELING AND DIFFERENT SAMPLES 244 PLANT
MATERIAL 244 PROTOCOL 244 DIAGRAM SHOWING POSITION OF ANTIBODIES ON
MULTIWELL SLIDE 246 APPENDIX 4 IMAGE PROCESSING WITH IMAGEJ 249
INTRODUCTION 249 DIFFERENT WINDOWS IN IMAGEJ 250 IMAGE LEVELS 250 COLORS
AND LOOK-UP TABLES 250 SIZE CALIBRATION 252 IMAGE MATH 252
QUANTIFICATION 254 STACKS AND 3D REPRESENTATION 254 FFT AND IMAGE
PROCESSING 256 MACRO LANGUAGE IN IMAGEJ 257 INDEX 259 |
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| id | DE-604.BV022219606 |
| illustrated | Illustrated |
| index_date | 2024-07-02T16:28:44Z |
| indexdate | 2024-07-20T07:52:42Z |
| institution | BVB |
| isbn | 0849339197 9780849339196 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-015430842 |
| oclc_num | 836300138 |
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| owner_facet | DE-19 DE-BY-UBM DE-703 DE-M49 DE-BY-TUM DE-188 DE-B16 DE-29T |
| physical | 268 S. zahlr. Ill. und graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | CRC Press |
| record_format | marc |
| spelling | Cox, Guy C. Verfasser aut Optical imaging techniques in cell biology Guy C. Cox Boca Raton, Fla. [u.a.] CRC Press 2007 268 S. zahlr. Ill. und graph. Darst. txt rdacontent n rdamedia nc rdacarrier Literaturangaben Cytology cabt Microscopy cabt Techniques cabt Cytologie (DE-588)4070177-3 gnd rswk-swf Mikroskopie (DE-588)4039238-7 gnd rswk-swf Cytologie (DE-588)4070177-3 s Mikroskopie (DE-588)4039238-7 s DE-604 HEBIS Datenaustausch Darmstadt application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015430842&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cox, Guy C. Optical imaging techniques in cell biology Cytology cabt Microscopy cabt Techniques cabt Cytologie (DE-588)4070177-3 gnd Mikroskopie (DE-588)4039238-7 gnd |
| subject_GND | (DE-588)4070177-3 (DE-588)4039238-7 |
| title | Optical imaging techniques in cell biology |
| title_auth | Optical imaging techniques in cell biology |
| title_exact_search | Optical imaging techniques in cell biology |
| title_exact_search_txtP | Optical imaging techniques in cell biology |
| title_full | Optical imaging techniques in cell biology Guy C. Cox |
| title_fullStr | Optical imaging techniques in cell biology Guy C. Cox |
| title_full_unstemmed | Optical imaging techniques in cell biology Guy C. Cox |
| title_short | Optical imaging techniques in cell biology |
| title_sort | optical imaging techniques in cell biology |
| topic | Cytology cabt Microscopy cabt Techniques cabt Cytologie (DE-588)4070177-3 gnd Mikroskopie (DE-588)4039238-7 gnd |
| topic_facet | Cytology Microscopy Techniques Cytologie Mikroskopie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015430842&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT coxguyc opticalimagingtechniquesincellbiology |