Generation of the Cntn2-IRES-EGFP BAC & Purkinje-specific CaMKII DKO mouse models: targeting the suppression of focal arrhythmogenesis in CHF
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Heidelberg
[2019]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 101 Seiten Illustrationen, Diagramme 30 cm |
Internformat
MARC
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| 100 | 1 | |a Chasan, Safak |e Verfasser |0 (DE-588)1193064724 |4 aut | |
| 245 | 1 | 0 | |a Generation of the Cntn2-IRES-EGFP BAC & Purkinje-specific CaMKII DKO mouse models |b targeting the suppression of focal arrhythmogenesis in CHF |c presented by M. Sc. Safak Chasan |
| 264 | 1 | |a Heidelberg |c [2019] | |
| 300 | |a 101 Seiten |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Ruperto Carola University Heidelberg |d 2019 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u http://d-nb.info/1192483669/04 |3 Inhaltsverzeichnis |
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Datensatz im Suchindex
| _version_ | 1804180425188311040 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
1
INTRODUCTION
................
................................................................................................................6
1.1
THE
DISCOVERY
AND
CHARACTERISTICS
OF
PURKINJE
CELLS
AND
THE
RELATION
OF
PURKINJE
CELLS
TO
VARIOUS
ARRHYTHMIAS6
1.2
CARDIAC
I
KI
CURRENT
AND
ITS
RELATION
TO
VARIOUS
ARRHYTHMIAS
12
1.3
CALCIUM
(CA
2+
)/CALMODULIN-DEPENDENT
PROTEIN
KINASE
II
(CAMKII)
AND
ITS
RELATION
TO
ARRHYTHMIAS
18
1.4
THE
RELATION
BETWEEN
I
KI
CURRENT
AND
CAMKII
BASED
ON
OUR
PRELIMINARY
RESULTS
22
1.5
OBJECTIVE
AND
HYPOTHESIS
24
2
MATERIALS
&
METHODS
26
2.1
GENERATION
AND
VERIFICATION
PROTOCOLS
OF
THE
RP24-266D16-ICRE-IRES
BAC
CLONE
BY
GENE
BRIDGES
GMBH
26
2.1.1
ARRIVAL
CHECK
ON
GENSAT
BAC
RP24-266D
16
BY
GENE
BRIDGES
GMBH
27
2.1.2
INSERTION
OF
THE
RPSL-NEO
COUNTERSELECTION
CASSETTE
BY
GENE
BRIDGES
GMBH
27
2.1.3
FUSION
OF
ICRE-IRES
ONTO
THE
ATG
OF
GENE
CNTN2
BY
GENE
BRIDGES
GMBH
28
2.1.4
ANALYSIS
OF
THE
BAC
DNA
BY
PULSED-FIELD
GEL
ELECTROPHORESIS
BY
GENE
BRIDGES
GMBH
28
2.2
GENERATION
AND
GENOTYPING
PROTOCOLS
OF
THE
CNTN2-IRES-EGFP
BAC
TRANSGENIC
REPORTER
MOUSE
28
2.2.1
PREPARATION
OF
LARGE
QUANTITY,
HIGH
QUALITY
LINEARIZED
BAC
DNA
FOR
PRONUCLEAR
INJECTION
(METHOD
PROVIDED
MONYER
LAB
OF
UNIVERSITY
OF
HEIDELBERG
AND
APPLIED
BY
IBF
OF
UNIVERSITY
OF
HEIDELBERG)
29
2.2.2
ISOLATION
OF
THE
DNA
FROM
THE
MOUSE
TISSUE
(TAIL
OR
EAR
TISSUE)
31
2.2.3
GENOTYPING
PROTOCOL
FOR
THE
CNTN2-IRES-EGFP
BAC
TRANSGENIC
REPORTER
MOUSE
32
2.3
VALIDATION
OF
THE
FUNCTIONALITY
OF
CRE
RECOMBINASE
BY
P-GALACTOSIDASE
(X-GAL)
STAINING
OF
HEART
TISSUES
FROM
CRE+
HETEROZYGOTE
ROSA26-LACZ
MICE
33
2.3.1
THE
BREEDING
CNTN2-IRES-EGFP
BAC
TRANSGENIC
MICE
WITH
ROSA26-LACZ
REPORTER
MICE
AND
THE
GENOTYPING
PROTOCOL
FOR
THE
CN2ROSA26
OFFSPRINGS
33
2.3.2
P-GALACTOSIDASE
(X-GAL)
STAINING
OF
THE
CRYOSECTIONS
OF
THE
HEART
TISSUES
OF
CN2ROSA26
MICE
36
2.4
CHARACTERIZATION
EXPERIMENTS
FOR
CNTN2-IRES-EGFP
BAC
TRANSGENIC
REPORTER
AND
PURKINJE-SPECIFIC
CAMKII
DKO
MOUSE
MODELS
37
2.4.1
ISOLATION
OF
PURKINJE
CELLS
WITH
A
MODIFIED
ADULT
CARDIOMYOCYTE
ISOLATION
PROTOCOL
37
2.4.2
IMMUNOFLUORESCENCE
(IF)
STAINING
PROTOCOL
&
FLUORESCENCE
MICROSCOPY
42
2.4.3
ECHOCARDIOGRAPHY
MEASUREMENTS
FOR
CNTN2-IRES-EGFP
BAC
TRANSGENIC
REPORTER
AND
PURKINJE-SPECIFIC
CAMKII
DKO
MOUSE
MODELS
43
2,4.4
ECG
TELEMETRY
TRANSMITTER
IMPLANTATION
AND
MEASUREMENTS
FOR
CNTN2-IRESEGFP
BAC
TRANSGENIC
REPORTER
MOUSE
MODEL
44
ECG
TELEMETRY
TRANSMITTER
IMPLANTATION:
44
MATERIALS:
44
1
ECG
MEASUREMENTS,
ANALYSIS
AND
STATISTICS:
.................................................................................................................................
45
2.5
GENERATION
AND
GENOTYPING
PROTOCOLS
OF
THE
PURKINJE-SPECIFIC
CAMKII
DKO
MOUSE
...................................
46
3
RESULTS
...................................................................................................................................................
50
3.1
GENERATION
AND
VERIFICATION
OF
THE
RP24-266D
1
6-ICRE-IRES
BAC
CLONE
........................................................
50
3.1.1
REPRESENTATION
OF
THE
SEQUENCE
OF
THE
CNTRI2
LOCUS
ON
GENSAT
BAC
RP24-266D
16
VS
ORIGINAL
BAC
RP24-266D
16
OF
MMRRC
AFTER
THE
ARRIVAL
CHECK
BY
GENE
BRIDGES
GMBH
....................................................................................................
50
3.1.2
PCR
ANALYSIS
AND
GRADIENT
PCR
VALIDATING
THE
RP24-266D16-ICRE-IRES
BACS
....................................................
52
3.1.3
ANALYSIS
OF
THE
BAC
DNA
BY
PULSED-FIELD
GEL
ELECTROPHORESIS
BY
GENE
BRIDGES
GMBH
.............................
55
3.2
GENERATION
OF
THE
CNTN2-IRES-EGFP
BAC
TRANSGENIC
REPORTER
MOUSE
...........................................................56
3.3
VALIDATION
OF
THE
FUNCTIONALITY
OF
CRE
RECOMBINASE
BY
P-GALACTOSIDASE
(X-GAL)
STAINING
OF
HEART
TISSUES
FROM
CRE+
HETEROZYGOTE
ROSA26-LACZ
MICE
.......................................................................................................................
57
3.3.1
GENOTYPING
RESULTS
OF
THE
BREEDING
CNTN2-IRES-EGFP
BAC
TRANSGENIC
MICE
WITH
ROSA26-LACZ
REPORTER
MICE
57
3.3.2
P-GALACTOSIDASE
(X-GAL)
STAINING
RESULTS
FROM
THE
CRYOSECTIONS
OF
THE
HEART
TISSUES
OF
CN2ROSA26
MICE
.............
57
3.4
CHARACTERIZATION
OF
THE
CNTN2-IRES-EGFP
BAC
TRANSGENIC
REPORTER
MOUSE
.................................................
59
3.4.1
IMMUNOFLUORESCENCE
CHARACTERIZATION
OF
THE
CNTN2-1RES-EGFP
BAC
TRANSGENIC
REPORTER
MOUSE
.......................
59
3.4.2
THE
ASSESSMENT
OF
THE
ECHOCARDIOGRAPHY
MEASUREMENTS
IN
CONTROL
VS
CNTN2-IRES-EGFP
BAC
MICE
...............
60
3.4.3
THE
ASSESSMENT
OF
THE
ECG
MEASUREMENTS
IN
CONTROL
VS
CNTN2-IRES-EGFP
BAC
MICE
.......................................
65
3.5
GENERATION
OF
THE
PURKINJE-SPECIFIC
CAMKII
DKO
MOUSE
..............................................................................
71
3.6
CHARACTERIZATION
OF
THE
PURKINJ
E-SPECIFIC
CAMKII
DKO
MOUSE
......................................................................
73
3.6.1
IMMUNOFLUORESCENCE
CHARACTERIZATION
OF
THE
PURKINJE-SPECIFIC
CAMKII
DKO
MOUSE
...........................................
73
3.6.2
THE
ASSESSMENT
OF
THE
ECHOCARDIOGRAPHY
MEASUREMENTS
IN
CONTROL
VS
PURKINJE-SPECIFIC
CAMKII
DKO
MICE
....
73
4
DISCUSSION..
*
------------
.............................................................
*
...................
*
.......
-------
.....79
5
CONCLUSION
AND
OUTLOOK
..........................
----------------------
...........................................................89
6
REFERENCES...............................
---------
..................................
-------------------
.......
--------
.................90
2
|
| any_adam_object | 1 |
| author | Chasan, Safak |
| author_GND | (DE-588)1193064724 |
| author_facet | Chasan, Safak |
| author_role | aut |
| author_sort | Chasan, Safak |
| author_variant | s c sc |
| building | Verbundindex |
| bvnumber | BV046110738 |
| ctrlnum | (OCoLC)1118993555 (DE-599)DNB1192483669 |
| discipline | Medizin |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV046110738 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T08:35:33Z |
| institution | BVB |
| language | English |
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| physical | 101 Seiten Illustrationen, Diagramme 30 cm |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| record_format | marc |
| spelling | Chasan, Safak Verfasser (DE-588)1193064724 aut Generation of the Cntn2-IRES-EGFP BAC & Purkinje-specific CaMKII DKO mouse models targeting the suppression of focal arrhythmogenesis in CHF presented by M. Sc. Safak Chasan Heidelberg [2019] 101 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Ruperto Carola University Heidelberg 2019 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1192483669/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031491339&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Chasan, Safak Generation of the Cntn2-IRES-EGFP BAC & Purkinje-specific CaMKII DKO mouse models targeting the suppression of focal arrhythmogenesis in CHF |
| subject_GND | (DE-588)4113937-9 |
| title | Generation of the Cntn2-IRES-EGFP BAC & Purkinje-specific CaMKII DKO mouse models targeting the suppression of focal arrhythmogenesis in CHF |
| title_auth | Generation of the Cntn2-IRES-EGFP BAC & Purkinje-specific CaMKII DKO mouse models targeting the suppression of focal arrhythmogenesis in CHF |
| title_exact_search | Generation of the Cntn2-IRES-EGFP BAC & Purkinje-specific CaMKII DKO mouse models targeting the suppression of focal arrhythmogenesis in CHF |
| title_full | Generation of the Cntn2-IRES-EGFP BAC & Purkinje-specific CaMKII DKO mouse models targeting the suppression of focal arrhythmogenesis in CHF presented by M. Sc. Safak Chasan |
| title_fullStr | Generation of the Cntn2-IRES-EGFP BAC & Purkinje-specific CaMKII DKO mouse models targeting the suppression of focal arrhythmogenesis in CHF presented by M. Sc. Safak Chasan |
| title_full_unstemmed | Generation of the Cntn2-IRES-EGFP BAC & Purkinje-specific CaMKII DKO mouse models targeting the suppression of focal arrhythmogenesis in CHF presented by M. Sc. Safak Chasan |
| title_short | Generation of the Cntn2-IRES-EGFP BAC & Purkinje-specific CaMKII DKO mouse models |
| title_sort | generation of the cntn2 ires egfp bac purkinje specific camkii dko mouse models targeting the suppression of focal arrhythmogenesis in chf |
| title_sub | targeting the suppression of focal arrhythmogenesis in CHF |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1192483669/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031491339&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT chasansafak generationofthecntn2iresegfpbacpurkinjespecificcamkiidkomousemodelstargetingthesuppressionoffocalarrhythmogenesisinchf |
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