Studies on the response of some Petunia genotypes to androgenesis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
Verlag Dr. Köster
2018
|
| Ausgabe: | 1. Auflage |
| Schriftenreihe: | Schriften zur internationalen Agrarentwicklung
Bd. 80 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Enthält Literaturangaben |
| Beschreibung: | XII, 213 Seiten Illustrationen, Diagramme |
| ISBN: | 9783895749049 3895749044 |
Internformat
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| 100 | 1 | |a Chaar, Mahmoud |d 1976- |e Verfasser |0 (DE-588)1156540542 |4 aut | |
| 245 | 1 | 0 | |a Studies on the response of some Petunia genotypes to androgenesis |c Mahmoud Chaar |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Berlin |b Verlag Dr. Köster |c 2018 | |
| 300 | |a XII, 213 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Schriften zur internationalen Agrarentwicklung |v Bd. 80 | |
| 500 | |a Enthält Literaturangaben | ||
| 502 | |b Dissertation |c Humboldt-Universität zu Berlin |d 2018 | ||
| 546 | |a Zusammenfassung auf Deutsch und Englisch | ||
| 650 | 0 | 7 | |a Antherenkultur |0 (DE-588)4333990-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Androgenese |0 (DE-588)4379476-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Blumenzüchtung |0 (DE-588)4493416-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Petunie |0 (DE-588)4173969-3 |2 gnd |9 rswk-swf |
| 653 | |a Androgenese | ||
| 653 | |a Biotechnologie | ||
| 653 | |a Gartenbau | ||
| 653 | |a Petunien | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Petunie |0 (DE-588)4173969-3 |D s |
| 689 | 0 | 1 | |a Blumenzüchtung |0 (DE-588)4493416-6 |D s |
| 689 | 0 | 2 | |a Androgenese |0 (DE-588)4379476-2 |D s |
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| 710 | 2 | |a Dr. Hans-Joachim Köster (Firma) |0 (DE-588)1065499345 |4 pbl | |
| 830 | 0 | |a Schriften zur internationalen Agrarentwicklung |v Bd. 80 |w (DE-604)BV008281241 |9 80 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-030308874 | ||
Datensatz im Suchindex
| _version_ | 1804178480559030272 |
|---|---|
| adam_text | TABLE OF CONTENTS
ACKNOWLEDGEMENTS........................................................................................................................
I
ABSTRACT........................................................................................................................................
ILL
ZUSAMMENFASSUNG.........................................................................................................................V
ABBREVIATIONS AND
DEFINITIONS...................................................................................................
VII
TABLE OF
CONTENTS.........................................................................................................................
IX
1
.INTRODUCTION.................................................................................................................................
1
2. LITERATURE
REVIEW....................................................................................................................6
2.1 THE GENUS
PETUNIA.............................................................................................................
6
2.2 IMPORTANCE OF PETUNIA IN FLOWER AND PLANT
MARKETS.........................................................9
2.3 BREEDING OF
PETUNIA.........................................................................................................
12
2.4 PLOIDY IN
PETUNIA.............................................................................................................
13
2.4.1
HAPLOIDY...................................................................................................................13
2.4.2
POLYPLOIDY...............................................................................................................
15
2.4.3
ANEUPLOIDY..............................................................................................................
16
2.5 HAPLOID
PRODUCTION..........................................................................................................
16
2.6
ANDROGENESIS...................................................................................................................
20
2.6.1 ANTHER
CULTURE..........................................................................................................22
2.6.2 ISOLATED MICROSPORE
CULTURE....................................................................................25
2.7 ANDROGENESIS IN
PETUNIA................................................................................................
28
2.8 FACTORS AFFECTING ANTHER CULTURE IN
PETUNIA....................................................................
32
2.8.1 EFFECT OF STRESS TYPES AND STRESS
PERIODS................................................................
32
2.8.2 EFFECT O F
GENOTYPE..................................................................................................
34
2.8.3 EFFECT OF COLLECTION TIM
E.........................................................................................
36
2.8.4 EFFECT OF PLANT*S AGE AND BUD
POSITIONS.................................................................
36
2.8.5 EFFECT OF DARK AND
LIGHT...........................................................................................
38
2.8.6 EFFECT OF BUD SIZE AND DEVELOPMENTAL STAGE OF MICROSPORES
...............................
39
2.8.7 EFFECT OF CULTURE MEDIA AND PLANT GROWTH REGULATORS
............................................
40
2.9 FACTORS AFFECTING ISOLATED MICROSPORE EMBRYOGENESIS IN
PETUNIA..................................43
3. AIMS AND
OBJECTIVES...........................................................................................................
44
3.1 RESEARCH
AIMS.................................................................................................................44
3.2 THE
OBJECTIVES..................................................................................................................
44
3.3
HYPOTHESES......................................................................................................................
46
4.MATERIALS AND M
ETHODS.............................................................................................................
48
4.1 PLANT
MATERIAL....................................................................................................................48
4.2 COLLECTION AND PREPARATION OF
MATERIAL...........................................................................50
4.2.1 COLLECTION OF FLOWER
BUDS......................................................................................
50
4.2.2 DISINFECTION OF
BUDS................................................................................................51
4.2.3 ISOLATION OF ANTHERS AND
MICROSPORES......................................................................52
4.3 STAINING
TECHNIQUE............................................................................................................55
4.4 CULTURE M
EDIA...................................................................................................................56
4.5 STRESS
PRE-TREATMENTS.......................................................................................................
58
4.6 CULTURE
TECHNIQUE............................................................................................................
58
4.7
ACCLIMATIZATION................................................................................................................60
4.8 ASSESSMENT OF PLOIDY
LEVEL..............................................................................................60
4.9 GENETIC ANALYSIS OF REGENERATED
PLANTS...........................................................................62
4.10 MORPHOLOGICAL
STUDIES.....................................................................................................
63
4.11 FACTORS AFFECTING ANTHER CULTURE IN PETUNIA
..................................................................
64
4.11.1 EFFECT OF COLLECTION TIM
ES.......................................................................................
64
4.11.2 EFFECT OF BUD
POSITION..............................................................................................64
4.11.3 EFFECT OF PLANT A G E
...................................................................................................65
4.11.4 EFFECT OF DARK AND LIGHT ON CALLUS FORMATION AND PLANT
REGENERATION
...................
65
4.11.5 EFFECT OF HARVEST PERIOD (YEAR TIMES) IN
PETUNIA...................................................66
4.11.6 EFFECT OF STRESS TYPE AND STRESS PERIOD
...................................................................
67
4.11.7 EFFECT O F BUD SIZE REPRESENTING MICROSPORE DEVELOPMENT STAGE
..........................
68
4.11.8 EFFECT OF MEDIA TYPE AND IMPROVING THE EFFICIENCY OF ANTHER
CULTURE IN LIQUID
MEDIUM..................................................................................................................................69
4.11.8.1 EFFECT OF MEDIUM
TYPES....................................................................................
69
4.11.8.2 IMPROVING THE EFFICIENCY OF ANTHER CULTURE IN LIQUID MEDIUM
.......................
69
4.11.9 EFFECTS OF PLANT GROWTH REGULATOR TYPE AND CONCENTRATION, PLANT
PLOIDY LEVEL, AND
GENOTYPE................................................................................................................................
70
4.12 FACTORS AFFECTING ISOLATED MICROSPORE EMBRYOGENESIS IN
PETUNIA...............................72
4.12.1 EFFECT OF ISOLATION METHOD AND MICROSPORE DENSITY
.............................................
72
4.12.2 EFFECT O F MICROSPORE DEVELOPMENT STAGE
...............................................................
74
4.12.3 EFFECT OF STRESS PRE-TREATMENT, GENOTYPES AND CULTURE MEDIA
..............................
75
4.12.4 ISOLATED MICROSPORE CULTURE FROM TETRAPLOID PLANTS
................................................
77
4.13 DATA COLLECTION AND STATISTICAL ANALYSIS
.........................................................................
77
4.14 OVERVIEW OF THE DIFFERENT EXPERIMENTS PERFORMED IN THE RESEARCH
.............................
78
5.RESULTS AND
DISCUSSION.............................................................................................................
83
5.1 DISINFECTION AND
PREPARATION..........................................................................................
83
5.1.1 DISINFECTION OF BUDS
...............................................................................................
83
5.1.2 POLLEN
VIABILITY........................................................................................................
85
5.1.3 DEVELOPMENT STAGE OF MICROSPORES RELATED TO BUD SIZE
........................................
87
5.2 CALLUS INDUCTION AND SHOOT REGENERATION
.......................................................................
90
5.3 FACTORS AFFECTING ANTHER CULTURE IN
PETUNIA..................................................................
95
5.3.1 EFFECT O F BUD COLLECTION TIME ON CALLUS FORMATION
................................................
96
5.3.2 EFFECT O F BUD POSITION ON CALLUS
FORMATION............................................................97
5.3.3 EFFECT O F DONOR PLANT AGE ON CALLUS FORMATION AND PLANT
REGENERATION
................
98
5.3.4 EFFECT OF DARK AND LIGHT ON CALLUS FORMATION AND PLANT
REGENERATION
.................
101
5.3.5 EFFECT O F HARVEST PERIOD (YEAR TIMES) IN
PETUNIA.................................................103
5.3.6 EFFECT OF STRESS TYPE AND STRESS PERIOD ON CALLUS FORMATION IN
PETUNIA
..............
104
5.3.6.1 EFFECT O F COLD STRESS ON CALLUS FORMATION IN PETUNIA
....................................
104
5.3.6.2 EFFECT OF STRESS TYPE AND STRESS PERIOD ON CALLUS
FORMATION..........................106
5.3.7 EFFECT OF MICROSPORE DEVELOPMENT STAGE (EFFECT O F BUD
SIZE)............................111
5.3.8 ASSESSMENT OF THE PLOIDY LEVEL IN PLANTS REGENERATED FROM ANTHER
CULTURE
......
114
5.3.9 EFFECT OF MEDIUM TYPES AND IMPROVING THE EFFICIENCY O F ANTHER
CULTURE IN LIQUID
MEDIUM...............................................................................................................................
120
5.3.9.1 EFFECT O F MEDIUM
TYPES..................................................................................
120
5.3.9.2 IMPROVING THE EFFICIENCY OF ANTHER CULTURE IN LIQUID MEDIUM
.....................
122
5.3.10 EFFECTS OF PLANT GROWTH REGULATORS, GENOTYPES AND PLANT PLOIDY
LEVEL ON CALLUS
FORMATION AND PLANT
REGENERATION......................................................................................
126
5.3.10.1 THE ROLE OF PLANT GROWTH REGULATOR COMBINATIONS, TYPE AND
CONCENTRATION 126
5.3.10.2 EFFECT O F GENOTYPE ON CALLUS
FORMATION......................................................... 130
5.3.10.3 EFFECT OF GENOTYPE ON THE EFFICIENCY O F CALLUS AND PLANT
REGENERATION
........
132
5.3.10.4 EFFECT OF PLANT PLOIDY LEVEL ON CALLUS FORMATION
...........................................
135
5.3.10.5 EFFECT OF PLANT PLOIDY LEVEL ON THE EFFICIENCY OF CALLUS AND
PLANT REGENERATION
..........................................................................................................................
137
5.3.10.6 EFFECT OF BA AND NAA CONCENTRATION IN ROOTING
MEDIUM...........................139
5.3.10.7 PLOIDY DISTRIBUTION IN REGENERATED PLANTS THROUGH ANTHER
CULTURE IN PETUNIA....
..........................................................................................................................
141
5.3.10.8 GENETIC ANALYSIS OF REGENERATED PLANTS
......................................................
151
5.3.11
ACCLIMATIZATION.....................................................................................................
157
5.3.12 MORPHOLOGICAL STUDIES (MORPHOLOGICAL CHARACTERISATION)
..................................
159
5.4 FACTORS AFFECTING ISOLATED MICROSPORE EMBRYOGENESIS IN PETUNIA
............................
165
5.4.1 EFFECT OF ISOLATION METHOD AND MICROSPORE DENSITY
...........................................
165
5.4.2 EFFECT OF MICROSPORE DEVELOPMENT STAGE
.............................................................
168
5.4.3 EFFECT OF STRESS TREATMENT, GENOTYPES AND CULTURE MEDIA
....................................
169
5.4.4 ISOLATED MICROSPORE CULTURE FROM TETRAPLOID
PLANTS..............................................172
6. GENERAL
DISCUSSION.............................................................................................................
173
7. CONCLUSION AND
RECOMMENDATIONS...................................................................................
180
8.
REFERENCES................................................................................................................................182
LIST OF
FIGURES..............................................................................................................................197
LIST OF
TABLES...............................................................................................................................
205
APPENDIX.....................................................................................................................................210
|
| any_adam_object | 1 |
| author | Chaar, Mahmoud 1976- |
| author_GND | (DE-588)1156540542 |
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| edition | 1. Auflage |
| format | Thesis Book |
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| spelling | Chaar, Mahmoud 1976- Verfasser (DE-588)1156540542 aut Studies on the response of some Petunia genotypes to androgenesis Mahmoud Chaar 1. Auflage Berlin Verlag Dr. Köster 2018 XII, 213 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Schriften zur internationalen Agrarentwicklung Bd. 80 Enthält Literaturangaben Dissertation Humboldt-Universität zu Berlin 2018 Zusammenfassung auf Deutsch und Englisch Antherenkultur (DE-588)4333990-6 gnd rswk-swf Androgenese (DE-588)4379476-2 gnd rswk-swf Blumenzüchtung (DE-588)4493416-6 gnd rswk-swf Petunie (DE-588)4173969-3 gnd rswk-swf Androgenese Biotechnologie Gartenbau Petunien (DE-588)4113937-9 Hochschulschrift gnd-content Petunie (DE-588)4173969-3 s Blumenzüchtung (DE-588)4493416-6 s Androgenese (DE-588)4379476-2 s Antherenkultur (DE-588)4333990-6 s DE-604 Dr. Hans-Joachim Köster (Firma) (DE-588)1065499345 pbl Schriften zur internationalen Agrarentwicklung Bd. 80 (DE-604)BV008281241 80 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030308874&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Chaar, Mahmoud 1976- Studies on the response of some Petunia genotypes to androgenesis Schriften zur internationalen Agrarentwicklung Antherenkultur (DE-588)4333990-6 gnd Androgenese (DE-588)4379476-2 gnd Blumenzüchtung (DE-588)4493416-6 gnd Petunie (DE-588)4173969-3 gnd |
| subject_GND | (DE-588)4333990-6 (DE-588)4379476-2 (DE-588)4493416-6 (DE-588)4173969-3 (DE-588)4113937-9 |
| title | Studies on the response of some Petunia genotypes to androgenesis |
| title_auth | Studies on the response of some Petunia genotypes to androgenesis |
| title_exact_search | Studies on the response of some Petunia genotypes to androgenesis |
| title_full | Studies on the response of some Petunia genotypes to androgenesis Mahmoud Chaar |
| title_fullStr | Studies on the response of some Petunia genotypes to androgenesis Mahmoud Chaar |
| title_full_unstemmed | Studies on the response of some Petunia genotypes to androgenesis Mahmoud Chaar |
| title_short | Studies on the response of some Petunia genotypes to androgenesis |
| title_sort | studies on the response of some petunia genotypes to androgenesis |
| topic | Antherenkultur (DE-588)4333990-6 gnd Androgenese (DE-588)4379476-2 gnd Blumenzüchtung (DE-588)4493416-6 gnd Petunie (DE-588)4173969-3 gnd |
| topic_facet | Antherenkultur Androgenese Blumenzüchtung Petunie Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030308874&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV008281241 |
| work_keys_str_mv | AT chaarmahmoud studiesontheresponseofsomepetuniagenotypestoandrogenesis AT drhansjoachimkosterfirma studiesontheresponseofsomepetuniagenotypestoandrogenesis |