Role of FAN in the autophagic response to Shigella flexneri infection:
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| 1. Verfasser: | |
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| Format: | Abschlussarbeit Mikrofilm Buch |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Ausgabe: | [Mikrofiche-Ausg.] |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 62 S. Ill., graph. Darst. |
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Datensatz im Suchindex
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| adam_text | CONTENTS
ZUSAMMENFASSUNG 2
ABSTRACT 3
ABBREVIATIONS 4
1 INTRODUCTION 7
1.1 AUTOPHAGY 7
1.1.1 GENERAL BACKGROUND 7
1.1.2 MOLECULAR MACHINERY 8
1.1.3 SUBSTRATE-SPECIFIC AUTOPHAGY 10
1.2 SHIGELLA FLEXNERI 12
1.2.1 SHIGELLA USES EFFECTOR PROTEINS TO MANIPULATE THE HOST CELL 13
1.2.2 SHIGELLA IS RECOGNIZED BY AUTOPHAGY 13
1.3 FAN - A MULTIFACETED PROTEIN 15
1.4 AIM OF THE STUDY 18
2 RESULTS 19
2.1 FAN LOCALIZES TO INTRACELLULAR SHIGELLA 19
2.2 FAN DEFICIENCY ENABLES ENHANCED BACTERIAL PROLIFERATION INSIDE
HOST CELLS 21
2.3 FAN COLOCALIZES WITH AUTOPHAGIC PROTEINS AND INFLUENCES THEIR
LOCALIZATION DURING S/I/GEWA-INFECTION 23
2.4 FAN DEFICIENCY ALTERS THE AUTOPHAGIC RESPONSE TO SHIGELLA INFECTION
25
2.5 FAN-DEPENDENT RESTRICTION OF INTRACELLULAR BACTERIAL PROLIFERATION
IS INDEPENDENT OF ICSB 28
2.6 FAN INTERACTS WITH BOTH LC3 AND GABARAP PROTEINS 29
2.7 FAN INTERACTS WITH LC3/GABARAP AND OTHER AUTOPHAGIC PRO
TEINS THROUGH THE WD REPEATS 30
2.8 FAN CONTAINS A SPECIFIC LC3 INTERACTING SITE 31
5
HTTP://D-NB.INFO/1062977335
CONTENTS
6
2.9 MUTATION OF THE POTENTIAL LIR-DOMAIN IMPAIRS THE RESCUE OF THE
FAN
-
/
-
PHENOTYPE 33
3 DISCUSSION 35
4 MATERIALS AND METHODS 42
4.1 CHEMICALS AND REAGENTS 42
4.2 BACTERIAL STRAINS AND CELL LINES 42
4.2.1 BACTERIA 42
4.2.2 CELL LINES 43
4.2.3 CELLULAR INFECTION WITH SHIGELLA 43
4.3 MICROSCOPY 44
4.3.1 COLOCALIZATION STUDIES 45
4.3.2 LIVE CELL MICROSCOPY 45
4.4 GENERATION, EXPRESSION AND DETECTION OF FAN CONSTRUCTS 46
4.4.1 DNA CONSTRUCTS 46
4.4.2 EXPRESSION IN EUKARYOTIC CELLS 47
4.5 SOFTWARE AND STATISTICAL ANALYSIS 49
DANKSAGUNGEN 63
LIST OF FIGURES 51
LIST OF TABLES 53
REFERENCES 54
DANKSAGUNGEN 63
ERKLARUNG 64
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| any_adam_object | 1 |
| author | Carstens, Anna Catharina 1982- |
| author_GND | (DE-588)105989503X |
| author_facet | Carstens, Anna Catharina 1982- |
| author_role | aut |
| author_sort | Carstens, Anna Catharina 1982- |
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| building | Verbundindex |
| bvnumber | BV042227120 |
| ctrlnum | (OCoLC)898059101 (DE-599)DNB1062977335 |
| discipline | Medizin |
| edition | [Mikrofiche-Ausg.] |
| format | Thesis Microfilm Book |
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| indexdate | 2024-07-10T01:15:48Z |
| institution | BVB |
| language | English |
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| physical | 62 S. Ill., graph. Darst. |
| publishDate | 2014 |
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| spelling | Carstens, Anna Catharina 1982- Verfasser (DE-588)105989503X aut Role of FAN in the autophagic response to Shigella flexneri infection vorgelegt von Anna Catharina Carstens [Mikrofiche-Ausg.] 2014 62 S. Ill., graph. Darst. txt rdacontent h rdamedia he rdacarrier Köln, Univ., Diss., 2014 Mikroform-Ausgabe Mikrofiche-Ausg.: 1 Mikrofiche : 24x (DE-588)4113937-9 Hochschulschrift gnd-content Reproduktion von Carstens, Anna Catharina, 1982- Role of FAN in the autophagic response to Shigella flexneri infection 2014 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027665510&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Carstens, Anna Catharina 1982- Role of FAN in the autophagic response to Shigella flexneri infection |
| subject_GND | (DE-588)4113937-9 |
| title | Role of FAN in the autophagic response to Shigella flexneri infection |
| title_auth | Role of FAN in the autophagic response to Shigella flexneri infection |
| title_exact_search | Role of FAN in the autophagic response to Shigella flexneri infection |
| title_full | Role of FAN in the autophagic response to Shigella flexneri infection vorgelegt von Anna Catharina Carstens |
| title_fullStr | Role of FAN in the autophagic response to Shigella flexneri infection vorgelegt von Anna Catharina Carstens |
| title_full_unstemmed | Role of FAN in the autophagic response to Shigella flexneri infection vorgelegt von Anna Catharina Carstens |
| title_short | Role of FAN in the autophagic response to Shigella flexneri infection |
| title_sort | role of fan in the autophagic response to shigella flexneri infection |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027665510&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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