Functional genomics in Medicago truncatula: methods and protocols
This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Tar...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1822 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab. Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field |
| Beschreibung: | Grain and forage legumes: nutritional value and agriculture sustainability -- Model legumes: functional genomics tools in Medicago truncatula -- The Medicago truncatula genome: genomic data availability -- Physical mutagenesis in Medicago truncatula using Fast Neutron Bombardment (FNB) for symbiosis and developmental biology studies -- Targeting Induced Local Lesions IN Genomes (TILLING) in Medicago truncatula -- T-DNA insertional mutagenesis and activation tagging in Medicago truncatula -- Tnt1 Insertional Mutagenesis in Medicago truncatula -- Transient post-transcriptional gene silencing in Medicago truncatula: Virus-Induced Gene Silencing (VIGS) -- Stable inactivation of microRNAs in Medicago truncatula roots -- Non-isotopic RNA in situ hybridization for functional analyses in Medicago truncatula -- In situ hybridization method for localization of mRNA molecules in Medicago tissue sections -- Editing the Medicago truncatula genome: targeted mutagenesis using the CRISPR-Cas9 reagent -- Functional genomics and seed development in Medicago truncatula: an overview -- Functional genomics and genetic control of compound leaf development in Medicago truncatula: an overview -- Root development in Medicago truncatula: lessons from genetics to functional genomics -- The multiple faces of the Medicago-Sinorhizobium symbiosis -- Functional genomics and flowering time in Medicago truncatula: an overview -- Functional genomics and genetic control of flower and fruit development in Medicago truncatula: an overview -- Towards unravelling the genetic determination of the acquisition of salt and osmotic stress tolerance through in vitro selection in Medicago truncatula -- Functional genomics in the study of metabolic pathways in Medicago truncatula: an overview |
| Beschreibung: | 1 Online-Ressource (XI, 343 Seiten) Illustrationen |
| ISBN: | 9781493986330 |
| DOI: | 10.1007/978-1-4939-8633-0 |
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| 520 | |a This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab. Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field | ||
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| spelling | Functional genomics in Medicago truncatula methods and protocols edited by Luis A. Cañas, José Pío Beltrán 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XI, 343 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1822 Grain and forage legumes: nutritional value and agriculture sustainability -- Model legumes: functional genomics tools in Medicago truncatula -- The Medicago truncatula genome: genomic data availability -- Physical mutagenesis in Medicago truncatula using Fast Neutron Bombardment (FNB) for symbiosis and developmental biology studies -- Targeting Induced Local Lesions IN Genomes (TILLING) in Medicago truncatula -- T-DNA insertional mutagenesis and activation tagging in Medicago truncatula -- Tnt1 Insertional Mutagenesis in Medicago truncatula -- Transient post-transcriptional gene silencing in Medicago truncatula: Virus-Induced Gene Silencing (VIGS) -- Stable inactivation of microRNAs in Medicago truncatula roots -- Non-isotopic RNA in situ hybridization for functional analyses in Medicago truncatula -- In situ hybridization method for localization of mRNA molecules in Medicago tissue sections -- Editing the Medicago truncatula genome: targeted mutagenesis using the CRISPR-Cas9 reagent -- Functional genomics and seed development in Medicago truncatula: an overview -- Functional genomics and genetic control of compound leaf development in Medicago truncatula: an overview -- Root development in Medicago truncatula: lessons from genetics to functional genomics -- The multiple faces of the Medicago-Sinorhizobium symbiosis -- Functional genomics and flowering time in Medicago truncatula: an overview -- Functional genomics and genetic control of flower and fruit development in Medicago truncatula: an overview -- Towards unravelling the genetic determination of the acquisition of salt and osmotic stress tolerance through in vitro selection in Medicago truncatula -- Functional genomics in the study of metabolic pathways in Medicago truncatula: an overview This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab. Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field Plant genetics Cañas, Luis ca. 20./21. Jh. (DE-588)1246786907 edt Beltrán, José Pío 1949- (DE-588)1246787512 edt Erscheint auch als Druck-Ausgabe 978-1-4939-8632-3 Erscheint auch als Druck-Ausgabe 978-1-4939-8634-7 Erscheint auch als Druck-Ausgabe 978-1-4939-9348-2 https://doi.org/10.1007/978-1-4939-8633-0 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Functional genomics in Medicago truncatula methods and protocols Plant genetics |
| title | Functional genomics in Medicago truncatula methods and protocols |
| title_auth | Functional genomics in Medicago truncatula methods and protocols |
| title_exact_search | Functional genomics in Medicago truncatula methods and protocols |
| title_exact_search_txtP | Functional genomics in Medicago truncatula methods and protocols |
| title_full | Functional genomics in Medicago truncatula methods and protocols edited by Luis A. Cañas, José Pío Beltrán |
| title_fullStr | Functional genomics in Medicago truncatula methods and protocols edited by Luis A. Cañas, José Pío Beltrán |
| title_full_unstemmed | Functional genomics in Medicago truncatula methods and protocols edited by Luis A. Cañas, José Pío Beltrán |
| title_short | Functional genomics in Medicago truncatula |
| title_sort | functional genomics in medicago truncatula methods and protocols |
| title_sub | methods and protocols |
| topic | Plant genetics |
| topic_facet | Plant genetics |
| url | https://doi.org/10.1007/978-1-4939-8633-0 |
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