Spatial aspects of enzymology: optimal arrangement and conformational dynamics
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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2013
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| Online-Zugang: | Volltext kostenfrei Inhaltsverzeichnis |
| Beschreibung: | XII, 102 S. Ill., graph. Darst. |
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Datensatz im Suchindex
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| adam_text | SPATIAL ASPECTS OF ENZYMOLOGY
OPTIMAL ARRANGEMENT AND CONFORMATIONAL DYNAMICS
ALEXANDER BUCHNER
DISSERTATION
AN DER FAKULTAT FUR PHYSIK
DER LUDWIG-MAXIMILIANS-UNIVERSITAT
MIINCHEN
VORGELEGT VON
ALEXANDER BUCHNER
AUS SIMBACH AM INN
MIINCHEN, DEN 25. OKTOBER 2013
HTTP://D-NB.INFO/1058376659
CONTENTS
LIST OF FIGURES VII
ZUSAMMENFASSUNG IX
ABSTRACT XI
1 INTRODUCTION 1
1.1 KINETICS OF ENZYMATIC REACTIONS 1
1.1.1 GENERAL PRINCIPLE 2
1.1.2 THE MICHAELIS-MENTEN KINETICS 2
1.1.3 THE CATALYTIC ACCELERATION OF ENZYMES 3
1.2 A STRUCTURAL VIEW ON ENZYMATIC REACTION 4
1.2.1 ACTIVE SITE 4
1.2.2 LOCK KEY 5
1.3 RNA BASED ENZYMES 6
1.4 PYRUVATE DEHYDROGENASE COMPLEX 6
1.4.1 THE COMPOSITION OF THE PYRUVATE DEHYDROGENASE COMPLEX 7
1.4.2 THE REACTION SCHEME OF THE PYRUVATE DEHYDROGENASE COMPLEX . . 8
1.5 OUTLINE OF THIS THESIS 10
2 KINETICS AND STRUCTURE OF A SINGLE ENZYME 11
2.1 ENZYME MEASUREMENT 12
2.1.1 THE ENZYME ASSAY METHOD 12
2.1.2 SINGLE MOLECULE MEASUREMENTS 12
2.2 FOLDING OF BIOMOLECULES 14
2.3 THE ENERGY LANDSCAPE 15
2.4 THE EXPERIMENTAL SETUP 16
2.5 A STOCHASTIC INVERSE PROBLEM IN SINGLE-MOLECULE ENZYMOLOGY 18
2.6 CONCLUSION 28
3 SPATIAL ARRANGEMENT OF ENZYMES 29
3.1 ADVANTAGE OF ENZYME CLUSTERING 30
3.2 METABOLIC CONTROL ANALYSIS 33
VI INHALTSVERZEICHNIS
3.3 ARTIFICIAL ENZYME CASCADES 34
3.3.1 DNA-SCAFFOLDS 34
3.3.2 THE DESIGN OF ARTIFICIAL ENZYME CASCADES 36
4 OPTIMAL ARRANGEMENT OF ENZYMES 39
4.1 A TWO ENZYME PATHWAY 39
4.1.1 LIMITS OF THE MM-REACTION 43
4.1.2 DIMENSIONALITY REDUCTION 43
4.2 MINIMAL MODEL 44
4.2.1 ENZYME EXPOSURE 47
4.2.2 COMPETING PATHWAY 52
4.3 CLUSTERING AND OPTIMAL ARRANGEMENT OF ENZYMES IN REACTION-DIFFUSION
SYS
TEMS 55
4.4 OPTIMIZATION OF COLLECTIVE ENZYME ACTIVITY VIA SPATIAL LOCALIZATION
.... 61
4.5 CONCLUSION 73
5 OUTLOOK 75
A SUPPLEMENTARY MATERIAL 79
A.L CLUSTERING AND OPTIMAL ARRANGEMENT OF ENZYMES IN REACTION-DIFFUSION
SYS
TEMS 79
A.2 OPTIMIZATION OF COLLECTIVE ENZYME ACTIVITY VIA SPATIAL LOCALIZATION
83
BIBLIOGRAPHY 91
DANKSAGUNG 102
|
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| spelling | Buchner, Alexander 1982- Verfasser (DE-588)1056898860 aut Spatial aspects of enzymology optimal arrangement and conformational dynamics Alexander Buchner 2013 XII, 102 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2013 Mit einer Zssfassung in dt. Sprache (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-173537 http://edoc.ub.uni-muenchen.de/17353/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-173537 Resolving-System kostenfrei DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027483922&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Buchner, Alexander 1982- Spatial aspects of enzymology optimal arrangement and conformational dynamics |
| subject_GND | (DE-588)4113937-9 |
| title | Spatial aspects of enzymology optimal arrangement and conformational dynamics |
| title_auth | Spatial aspects of enzymology optimal arrangement and conformational dynamics |
| title_exact_search | Spatial aspects of enzymology optimal arrangement and conformational dynamics |
| title_full | Spatial aspects of enzymology optimal arrangement and conformational dynamics Alexander Buchner |
| title_fullStr | Spatial aspects of enzymology optimal arrangement and conformational dynamics Alexander Buchner |
| title_full_unstemmed | Spatial aspects of enzymology optimal arrangement and conformational dynamics Alexander Buchner |
| title_short | Spatial aspects of enzymology |
| title_sort | spatial aspects of enzymology optimal arrangement and conformational dynamics |
| title_sub | optimal arrangement and conformational dynamics |
| topic_facet | Hochschulschrift |
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