The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research in the 21st century. In Apoptosis Methods and Protocols, highly experienced researchers in the field of apoptosis describe in step-by-step...

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Bibliographische Detailangaben

Weitere Verfasser:	Brady, Hugh J. M. (HerausgeberIn)
Format:	Elektronisch E-Book
Sprache:	English
Veröffentlicht:	Totowa, NJ Humana Press 2004
Schriftenreihe:	Methods in Molecular Biology 282
Schlagworte:	Life Sciences Cell Biology Life sciences Cell biology Labortechnik Apoptosis Aufsatzsammlung
Online-Zugang:	UBR01 TUM01 URL des Erstveröffentlichers
Zusammenfassung:	The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research in the 21st century. In Apoptosis Methods and Protocols, highly experienced researchers in the field of apoptosis describe in step-by-step detail the techniques they have perfected to investigate the critical steps involved in the apoptotic process. These readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Apoptosis Methods and Protocols constitutes a key technical reference to the significant methodologies used in the field, as well as offering novice and experienced researchers alike powerful tools to illuminate the phenomenon of programmed cell death
Beschreibung:	1 Online-Ressource (XI, 304 p. 74 illus)
ISBN:	9781592598120
DOI:	10.1385/1592598129