Chicken Egg Yolk Antibodies, Production and Application: IgY-Technology
Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals, especially rabbits. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an...
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Format: | Elektronisch E-Book |
Sprache: | English |
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Berlin, Heidelberg
Springer Berlin Heidelberg
2001
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Schriftenreihe: | Springer Lab Manuals
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Online-Zugang: | UBR01 Volltext |
Zusammenfassung: | Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals, especially rabbits. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Since hens can be kept under nearly natural conditions and antibodies be isolated from the collected eggs, this technology has become an interesting alternative to the ,bloody" techniques. For their work on yolk antibodies, the authors of this book received the FISEA prize (International Foundation for the Substitution of Animal Experimentation, Luxembourg) in 1997. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual. These practical instructions are complemented by a short introduction to the hen's humoral immune system and a section on the pros and cons of chicken yolk antibodies compared to those of mammals'serum. Avian antibodies have been shown to be in some applications even more effective than mamalian antibodies, especially when phylogenetically highly conserved antigens have been used for immunisation |
Beschreibung: | 1 Online-Ressource (XII, 255 p. 36 illus., 3 illus. in color) |
ISBN: | 9783662044889 |
DOI: | 10.1007/978-3-662-04488-9 |
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520 | |a Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals, especially rabbits. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Since hens can be kept under nearly natural conditions and antibodies be isolated from the collected eggs, this technology has become an interesting alternative to the ,bloody" techniques. For their work on yolk antibodies, the authors of this book received the FISEA prize (International Foundation for the Substitution of Animal Experimentation, Luxembourg) in 1997. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual. These practical instructions are complemented by a short introduction to the hen's humoral immune system and a section on the pros and cons of chicken yolk antibodies compared to those of mammals'serum. Avian antibodies have been shown to be in some applications even more effective than mamalian antibodies, especially when phylogenetically highly conserved antigens have been used for immunisation | ||
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Datensatz im Suchindex
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any_adam_object | |
author2 | Schade, Rüdiger Behn, Ingrid Erhard, Michael Hlinak, Andreas |
author2_role | edt edt edt edt |
author2_variant | r s rs i b ib m e me a h ah |
author_facet | Schade, Rüdiger Behn, Ingrid Erhard, Michael Hlinak, Andreas |
building | Verbundindex |
bvnumber | BV046148874 |
classification_rvk | XD 3111 |
collection | ZDB-2-SBL |
ctrlnum | (ZDB-2-SBL)978-3-662-04488-9 (OCoLC)1119020656 (DE-599)BVBBV046148874 |
dewey-full | 660.6 |
dewey-hundreds | 600 - Technology (Applied sciences) |
dewey-ones | 660 - Chemical engineering |
dewey-raw | 660.6 |
dewey-search | 660.6 |
dewey-sort | 3660.6 |
dewey-tens | 660 - Chemical engineering |
discipline | Chemie / Pharmazie Medizin |
doi_str_mv | 10.1007/978-3-662-04488-9 |
format | Electronic eBook |
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indexdate | 2024-07-10T08:36:35Z |
institution | BVB |
isbn | 9783662044889 |
language | English |
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physical | 1 Online-Ressource (XII, 255 p. 36 illus., 3 illus. in color) |
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spelling | Chicken Egg Yolk Antibodies, Production and Application IgY-Technology edited by Rüdiger Schade, Ingrid Behn, Michael Erhard, Andreas Hlinak, Christian Staak Berlin, Heidelberg Springer Berlin Heidelberg 2001 1 Online-Ressource (XII, 255 p. 36 illus., 3 illus. in color) txt rdacontent c rdamedia cr rdacarrier Springer Lab Manuals Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals, especially rabbits. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Since hens can be kept under nearly natural conditions and antibodies be isolated from the collected eggs, this technology has become an interesting alternative to the ,bloody" techniques. For their work on yolk antibodies, the authors of this book received the FISEA prize (International Foundation for the Substitution of Animal Experimentation, Luxembourg) in 1997. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual. These practical instructions are complemented by a short introduction to the hen's humoral immune system and a section on the pros and cons of chicken yolk antibodies compared to those of mammals'serum. Avian antibodies have been shown to be in some applications even more effective than mamalian antibodies, especially when phylogenetically highly conserved antigens have been used for immunisation Biotechnology Immunology Molecular Medicine Cell Biology Medicine Cytology Immunglobulin Y (DE-588)4327192-3 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Hühnerei (DE-588)4026050-1 gnd rswk-swf Labormedizin (DE-588)4073931-4 gnd rswk-swf Antikörper (DE-588)4002290-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Hühnerei (DE-588)4026050-1 s Antikörper (DE-588)4002290-0 s Immunglobulin Y (DE-588)4327192-3 s Methode (DE-588)4038971-6 s DE-604 Labormedizin (DE-588)4073931-4 s Schade, Rüdiger edt Behn, Ingrid edt Erhard, Michael edt Hlinak, Andreas edt Erscheint auch als Druck-Ausgabe 9783662044902 Erscheint auch als Druck-Ausgabe 9783662044896 Erscheint auch als Druck-Ausgabe 9783540666790 https://doi.org/10.1007/978-3-662-04488-9 Verlag URL des Erstveröffentlichers Volltext |
spellingShingle | Chicken Egg Yolk Antibodies, Production and Application IgY-Technology Biotechnology Immunology Molecular Medicine Cell Biology Medicine Cytology Immunglobulin Y (DE-588)4327192-3 gnd Methode (DE-588)4038971-6 gnd Hühnerei (DE-588)4026050-1 gnd Labormedizin (DE-588)4073931-4 gnd Antikörper (DE-588)4002290-0 gnd |
subject_GND | (DE-588)4327192-3 (DE-588)4038971-6 (DE-588)4026050-1 (DE-588)4073931-4 (DE-588)4002290-0 (DE-588)4143413-4 |
title | Chicken Egg Yolk Antibodies, Production and Application IgY-Technology |
title_auth | Chicken Egg Yolk Antibodies, Production and Application IgY-Technology |
title_exact_search | Chicken Egg Yolk Antibodies, Production and Application IgY-Technology |
title_full | Chicken Egg Yolk Antibodies, Production and Application IgY-Technology edited by Rüdiger Schade, Ingrid Behn, Michael Erhard, Andreas Hlinak, Christian Staak |
title_fullStr | Chicken Egg Yolk Antibodies, Production and Application IgY-Technology edited by Rüdiger Schade, Ingrid Behn, Michael Erhard, Andreas Hlinak, Christian Staak |
title_full_unstemmed | Chicken Egg Yolk Antibodies, Production and Application IgY-Technology edited by Rüdiger Schade, Ingrid Behn, Michael Erhard, Andreas Hlinak, Christian Staak |
title_short | Chicken Egg Yolk Antibodies, Production and Application |
title_sort | chicken egg yolk antibodies production and application igy technology |
title_sub | IgY-Technology |
topic | Biotechnology Immunology Molecular Medicine Cell Biology Medicine Cytology Immunglobulin Y (DE-588)4327192-3 gnd Methode (DE-588)4038971-6 gnd Hühnerei (DE-588)4026050-1 gnd Labormedizin (DE-588)4073931-4 gnd Antikörper (DE-588)4002290-0 gnd |
topic_facet | Biotechnology Immunology Molecular Medicine Cell Biology Medicine Cytology Immunglobulin Y Methode Hühnerei Labormedizin Antikörper Aufsatzsammlung |
url | https://doi.org/10.1007/978-3-662-04488-9 |
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