The role of Ceramide-1-Phosphate in pulmonary inflammation:
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XX, 170 S. Ill., graph. Darst. 30 cm |
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Datensatz im Suchindex
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| adam_text | THE ROLE OF CERAMIDE-1-PHOSPHATE
IN PULMONARY INFLAMMATION
O
CC
D
INAUGURALDISSERTATION
ZUR ERLANGUNG DES DOKTORGRADES
DER FAKULTAT FUR CHEMIE UND PHARMAZIE
DER ALBERT-LUDWIGS-UNIVERSITAT FREIBURG IM BREISGAU
VORGELEGT VON
KRISTIN BAUDIFT
AUS LEIPZIG
DEZEMBER 2014
HTTP://D-NB.INFO/1065827717
TABLE OF CONTENT
LIST OF FIGURES........
XII .
LIST
OF
TABLES
XV
LIST OF ABBREVIATIONS
XVI
1 ABSTRACT 1
2 INTRODUCTION..,.. 5
2.1 CERAMIDE-1
-PHOSPHATE 6
2.2 LUNG DISEASES... 11
2.2.1 COPD :....11
2.2.1.1 DEFINITION 11
2.2.1.2 RISK FACTORS, DIAGNOSIS AND SYMPTOMS.. 12
2.2.1.3 PATHOPHYSIOLOGY 13
2.2.2 ACUTE LUNG INJURY 17
2.2.2.1 DEFINITION 17
2.2.2.2 CAUSES, ENVIRONMENTAL INFLUENCES AND SYMPTOMS 19
2.2.2.3 PATHOPHYSIOLOGIC MECHANISM IN ALI 20
2.2.3 ASTHMA 23
2.2.3.1 DEFINITION !... 23
2.2.3.2 RISK FACTORS, DIAGNOSIS AND SYMPTOMS 24
2.2.3.3 PATHOPHYSIOLOGY 26
2.3 OBJECTIVES OF THE STUDY 29
3 RESULTS 31
. 3.1 THE EFFECT OF C1P IN COPD 32
VIII
3.1.1 EXOGENOUS C1P INHIBITS CS-INDUCED LUNG INFLAMMATION IN AN
ACUTE AND ONGOING SMOKE MODEL 32
3.1.2 C1P PARTIALLY PREVENTS THE DEVELOPMENT OF EMPHYSEMA IN A
CHRONIC MODEL OF CS-INDUCED LUNG INFLAMMATION 34
3.1.3 C1P REDUCES CS-INDUCED NF-KB DNA BINDING AND MRNA
EXPRESSION IN LUNG TISSUE AND HUMAN AIRWAY EPITHELIAL CELL LINES 40
3.1.4 EFFECT OF C1P ON CS-INDUCED CYTOKINE PRODUCTION IN HUMAN
AIRWAY EPITHELIAL CELL LINES ..43
3.1.5 C1P ATTENUATES CSE-INDUCED NF-KB EXPRESSION AND INHIBITS IL-8
PRODUCTION IN HUMAN NEUTROPHILS 44
3.1.6 THE MODULATORY EFFECT OF C1P ON NSMASE IN MURINE LUNG TISSUE
AND IN CS-EXPOSED HUMAN GRANULOCYTES AND SERA 48
3.2 THE INFLUENCE OF C1P IN ACUTE LUNG INJURY :...52
3.2.1 THE PROPHYLACTIC EFFECT OF C1P ON LPS-INDUCED ALI 52
3.2.2 THE THERAPEUTIC EFFECT OF C1P ON LPS-INDUCED ALI 54
3.2.3 C1P DECREASED LPS-INDUCED PLASMA LEAKAGE 55
3.2.4 RECRUITMENT OF NEUTROPHILS IN THE LUNG PARENCHYMA AFFECTED BY
C1P 56
3.2.5 C1P REDUCED PHOSPHORYLATION OF NF-KB P65 EXPRESSION IN LUNG
TISSUE 58
3.2.6 C1P ATTENUATED MRNA NF-KB2 EXPRESSION IN LUNG TISSUE 60
3.2.7 EFFECTS OF C1P ON LPS-INDUCED IL-8 PRODUCTION AND NF-KB
ACTIVATION IN NEUTROPHILS..! 63
3.3 THE ROLE OF C1P IN ASTHMA 65
3.3.1 C1P ATTENUATES OVA-INDUCED AAI. 65
3.3.2 C1P ADMINISTRATION PREVENTS INFLAMMATION IN HDM-INDUCED AAI 67
3.3.3 C1P TREATMENT REDUCED BRONCHIAL HYPERRESPONSIVENESS 69
3.3.4 C1P AFFECTS DC FUNCTION
IN VITRO
AND
IN VIVO
.....71
3.3.5 C1P REDUCED ALLERGEN-INDUCED NF-KB DNA BINDING AND
INFLUENCES DC FUNCTION BY MODULATING NF-KB, MAPK AND AKT 75
IX
3.3.6 C1P STIMULATION OF HUMAN MODC
IN VITRO
REDUCED TNF-A AND
IL-12 LEVEL AND INCREASED IL-10 PRODUCTION 78
4 DISCUSSION 79
4.1 C1P DECREASED THE INFLAMMATION OF COPD 80
4.2 PROTECTION OF ALI BY TREATMENT WITH C1P...... 86
4.3 THE ANTI-INFLAMMATORY EFFECT OF C1P ON ASTHMA 90
5 SUMMARY 95
6 ZUSAMMENFASSUNG 99
7 MATERIAL AND METHODS 103
7.1 MATERIAL :.. 104
7.1.1 PREPARATION OF C1P AND CSE 104
7.1.2 CHEMICALS 104
7.1.3 ANTIBODIES, CYTOKINES, ENZYMES AND KITS....:..: 107
7.1.4 BUFFER 109
7.1.5 PRIMERS 112
7.1.6 CONSUMABLES 113
7.1.7 EQUIPMENT 115
7.1.8 SOFTWARE 116
7.2 MICE MODELS.. 117
7.2.1 CS-INDUCED ACUTE LUNG INFLAMMATION 117
7.2.2 CS-INDUCED ONGOING LUNG INFLAMMATION 118
7.2.3 CS-INDUCED CHRONIC LUNG INFLAMMATION AND EMPHYSEMA 118
7.2.4 LPS MODEL OF ACUTE LUNG INFLAMMATION :.... 118
7.2.5 ACUTE OVALBUMIN/ALUM MODEL OF AAI 120
7.2.6 HDM-INDUCED AAI 121
7.2.7 AAL-INDUCED BY ADOPTIVE TRANSFER OF DC 122
X
7.2.8 T-CELL PRIMING CAPACITY OF MOUSE BMDCS
IN VITRO
AND
IN VIVO
123
7.3 METHODS....,: 123
7.3.1 CELL CULTURE . .123
7.3.1.1 HUMAN AIRWAY EPITHELIAL CELLS 123
7.3.1.2 EXAMINATION OF CELL VIABILITY 124
7.3.1.3 ISOLATION AND CULTIVATION OF BLOOD CELLS 124
7.3.1.4 ISOLATION OF BMDC 126
7.3.1.5 ISOLATION OF CD4
+
T CELLS 126
7.3.2 RECRUITMENT OF PATIENTS AND THEIR CHARACTERISTICS 127
7.3.3 STIMULATION OF CELLS FOR ELISA 128
7.3.4 PROTEIN DETECTION METHODS 130
7.3.4.1 ENZYME-LINKED IMMUNOSORBANT ASSAY 130
7.3.4.2 SPHINGOMYELINASE FLUOROMETRIC ASSAY 131
7.3.4.3 WESTERN BLOT 133
7.3.5 MEASUREMENT OF NF-KB DNA BINDING BY EMSA 137
7.3.6 FLOW CYTOMETRY 139
7.3.7 IN SITU-IMMUNOLOKALISATION 140
7.3.7.1 CRYOSTAT FIXATION 140
7.3.7.2 PARAFFIN EMBEDDING ...141
7.3.8 GENE EXPRESSION METHODS 143
7.3.8.1 RNA ISOLATION 143
7.3.8.2 CDNA SYNTHESIS 144
7.3.8.3 QUANTITATIVE REAL TIME PCR 144
7.3.9 STATISTICAL ANALYSIS 145
8 ACKNOWLEDGEMENT ........147
9 REFERENCE LIST 151
XI
|
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| author | Baudiß, Kristin |
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| spelling | Baudiß, Kristin Verfasser (DE-588)1065321546 aut The role of Ceramide-1-Phosphate in pulmonary inflammation vorgelegt von Kristin Baudiß 2014 XX, 170 S. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Freiburg (Breisgau), Univ., Diss., 2014 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027810010&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Baudiß, Kristin The role of Ceramide-1-Phosphate in pulmonary inflammation |
| subject_GND | (DE-588)4113937-9 |
| title | The role of Ceramide-1-Phosphate in pulmonary inflammation |
| title_auth | The role of Ceramide-1-Phosphate in pulmonary inflammation |
| title_exact_search | The role of Ceramide-1-Phosphate in pulmonary inflammation |
| title_full | The role of Ceramide-1-Phosphate in pulmonary inflammation vorgelegt von Kristin Baudiß |
| title_fullStr | The role of Ceramide-1-Phosphate in pulmonary inflammation vorgelegt von Kristin Baudiß |
| title_full_unstemmed | The role of Ceramide-1-Phosphate in pulmonary inflammation vorgelegt von Kristin Baudiß |
| title_short | The role of Ceramide-1-Phosphate in pulmonary inflammation |
| title_sort | the role of ceramide 1 phosphate in pulmonary inflammation |
| topic_facet | Hochschulschrift |
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