BK channels in human glioma cells:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2003
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Jena, Univ., Diss., 2003 |
| Beschreibung: | 94 Bl. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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1 INTRODUCTION 9
1.1 PATHOLOGY AND EPIDEMIOLOGY OF GLIOMAS 9
1.2 FOCUS OF GLIOMA RESEARCH 10
1.2.1 VOLTAGE-GATED ION CHANNELS IN HUMAN GLIOMAS 11
NA+CHANNELS 12
K
+CHANNELS 12
1.2.2 VOLTAGE-GATED K
+ CHANNELS AND CELL PROLIFERATION 13
GLIOMA CELLS 15
1.2.3 VOLTAGE-GATED K+ CHANNELS AND CELL MIGRATION 16
GLIOMA CELLS 18
1.3 BK CHANNELS - STRUCTURE, PHARMACOLOGY AND REGULATION 21
1.4 EPIDERMAL GROWTH FACTOR ( EGF) IN GLIOMAS - ROLE FOR CELL
PROLIFERATION,
CELL MOTILITY AND ACTIVATION OF BK CHANNELS 24
PROLIFERATION 24
MOTILITY 25
2 AIM OF THE STUDY 26
3 MATERIALS AND METHODS ~ 27
3.1 CELL CULTURE 27
3.2 PROLIFERATION ASSAY 27
3.3 ELECTROPHYSIOLOGICAL INVESTIGATIONS 28
3.3.1 WHOLE-CELL RECORDINGS 28
3.3.2 SINGLE-CHANNEL RECORDINGS 28
3.3.3 EXCISED-PATCH RECORDINGS 29
3.3.4 DATA ANALYSIS 29
3.4 MIGRATION STUDIES 31
3.4.1 BOYDEN-CHAMBER MIGRATION ASSAY 31
3.4.2 TIME-LAPSE VIDEOMICROSCOPY 32
3.5 STATISTICAL ANALYSIS 32
4 RESULTS 33
4.1 THE HUMAN ASTROCYTOMA 1321 N1 CELL LINE DISPLAY DIFFERENT
MORPHOLOGICAL CELL TYPES IN CULTURE 33
4.2 HUMAN ASTROCYTOMA 1321N1 CELLS SHOW BK CURRENTS, WHICH ARE ACTIVE
UNDER PHYSIOLOGICAL CONDITIONS 33
4.2.1 WHOLE-CELL RECORDINGS 33
4.2.2 SINGLE-CHANNEL RECORDINGS 38
CELL-ATTACHED RECORDINGS 38
EXCISED-PATCH RECORDINGS 40
4.3 CHRONIC EXPOSURE TO EGF DEPOLARIZES 1321N1 CELLS AND
K+OUTWARD CURRENTS 42
4.4 UNDER PARTICULAR CONDITIONS SPECIFIC BK CHANNEL BLOCKERS INHIBIT
PROLIFERATION OF 1321 N1 ASTROCYTOMA CELLS 42
4.5 SPECIFIC BLOCK OF BK CHANNELS DOES NOT INFLUENCE MIGRATION
OF 1321 N1 ASTROCYTOMA CELLS 44
4.5.1 BOYDEN CHAMBER MIGRATION ASSAY 44
4.5.2 TIME LAPSE VIDEOMICROSCOPY 46
2
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/967266505
5 DISCUSSION 49
5.1 BK CHANNELS IN NORMAL AND NEOPLASTIC CELLS 49
5.2 PROPERTIES OF BK CHANNELS IN 1321N1 HUMAN ASTROCYTOMA CELLS 50
5.2.1 CA2+ - AND VOLTAGE-DEPENDENCE OF BK CHANNELS IN 1321N1 HUMAN
ASTROCYTOMA CELLS 50
5.2.2 PHARMACOLOGY OF BK CHANNELS 53
5.2.3 BK CHANNELS IN 1321N1 HUMAN ASTROCYTOMA CELLS ARE ACTIVE UNDER
PHYSIOLOGICAL CONDITIONS 54
5.3 BK CHANNELS AND THEIR ROLE IN IN CELL PROLIFERATION 57
5.3.2 BLOCKADE OF BK CHANNELS INFLUENCES PROLIFERATION ONLY
UNDER PARTICULAR CONDITIONS 57
K+ - INDUCED PROLIFERATION 58
GROWTH FACTOR-STIMULATED PROLIFERATION 59
5.3.3 POSSIBLE MECHANISMS OF INVOLVEMENT OF BK CHANNELS IN
CELL PROLIFERATION 60
5.3.4 BK CHANNELS AND THEIR ROLE IN CELL MIGRATION 61
5.4 EFFECT OF EGF ON BK CHANNELS 66
6 CONCLUSIONS - 69
7 SUMMARY 71
8 REFERENCES 73
9 APPENDIX 90
9.1 PUBLICATIONS 90
9.2 ACKNOWLEDGMENT 91
9.2 CURRICULUM VITAE 92
9.3 EIDESTATTLICHE ERKLARUNG 93
9.4 ZUSAMMENFASSUNG (DEUTSCH) 94
3 |
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| indexdate | 2024-11-22T17:36:51Z |
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| language | English |
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| spelling | Basrai, Daniel Verfasser (DE-588)124455697 aut BK channels in human glioma cells von Daniel Basrai 2003 94 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Jena, Univ., Diss., 2003 Gliom Ionenkanal Physiologie Spannungsabhängigkeit Zelllinie (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010281058&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Basrai, Daniel BK channels in human glioma cells Gliom Ionenkanal Physiologie Spannungsabhängigkeit Zelllinie |
| subject_GND | (DE-588)4113937-9 |
| title | BK channels in human glioma cells |
| title_auth | BK channels in human glioma cells |
| title_exact_search | BK channels in human glioma cells |
| title_full | BK channels in human glioma cells von Daniel Basrai |
| title_fullStr | BK channels in human glioma cells von Daniel Basrai |
| title_full_unstemmed | BK channels in human glioma cells von Daniel Basrai |
| title_short | BK channels in human glioma cells |
| title_sort | bk channels in human glioma cells |
| topic | Gliom Ionenkanal Physiologie Spannungsabhängigkeit Zelllinie |
| topic_facet | Gliom Ionenkanal Physiologie Spannungsabhängigkeit Zelllinie Hochschulschrift |
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