Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2007
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VI, 105 Bl. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV023400911 | ||
| 003 | DE-604 | ||
| 005 | 20090210 | ||
| 007 | t | ||
| 008 | 080717s2007 ad|| m||| 00||| eng d | ||
| 035 | |a (OCoLC)244072972 | ||
| 035 | |a (DE-599)BSZ277504996 | ||
| 040 | |a DE-604 |b ger | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 | ||
| 082 | 0 | |a 573.1554 |2 22/ger | |
| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a Bartels, Susanne |d 1980- |e Verfasser |0 (DE-588)134137817 |4 aut | |
| 245 | 1 | 0 | |a Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit |c vorgelegt von Susanne Bartels |
| 264 | 1 | |c 2007 | |
| 300 | |a VI, 105 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Ulm, Univ., Diss., 2008 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016583700&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016583700 | ||
Datensatz im Suchindex
| _version_ | 1804137783723294720 |
|---|---|
| adam_text | TABLE OF CONTENTS
LIST OF ABBREVIATIONS PAGE III
1. INTRODUCTION l__
1.1 Hematopoiesis 1
1.1.1 Hematopoietic Stem Cells (HSC) 1
1.1.2 Hematopoietic Development 3
1.1.3 The Stem Cell Niche 10
1.2 The Receptor Tyrosine Kinase Kit 14
1.3 Stem Cell Mobilization 17
1.4 Background and Objective of this Thesis 19
2. MATERIAL AND METHODS 20_
2.1 Material 20
2.1.1 Buffers and Solutions 20
2.1.2 Enzymes 21
2.1.3 Growth Factors 21
2.1.4 Antibodies and Second Steps 21
2.1.5 Oligonucleotides 21
2.1.6 Cell Culture Media and Supplements 22
2.1.7 Chemicals 22
2.1.8 Plastic Ware and Other Consumables 23
2.2 Methods 24
2.2.1 Mouse Strains and Breeding 24
2.2.2 Treatment of Mice with Cyclophosphamide (Endoxan) 26
2.2.3 Treatment of Mice with rhG-CSF (Pegfilgrastim) 26
2.2.4 Preparation of Organs and Cell Suspensions 27
2.2.5 Determination of White Blood Cell (WBC) Numbers 27
2.2.6 Sorting Non-Erythroid Cells 27
2.2.7 Methylcellulose Assays 28
2.2.8 Phenotypic Analysis by Flow Cytometry 29
2.2.9 Cytospins and Histological Staining 31
2.2.10 Statistical Analysis 31
3. RESULTS 32_
3.1 Treatment with Cyclophosphamide (Endoxan) 32
3.1.1 Mobilization of HSC by Cyclophosphamide 3 3
3.1.2 Mobilization of HPC by Cyclophosphamide 41
3.1.2 Mobilization of Neutrophils by Cyclophosphamide 45
I
3.2 Treatment with rhG-CSF (Pegfilgrastim) 51
3.2.1 Mobilization of HSC by rhG-CSF 52
3.2.2 Mobilization of HPC by rhG-CSF 56
3.2.3 Mobilization of Neutrophils by rhG-CSF 59
4. DISCUSSION 66_
4.1 Mobilization of HSC and HPC 67
4.2 Mobilization of Neutrophil Granulocytes 70
4.3 CY- versus rhG-CSF-mediated Mobilization 71
5. SUMMARY 74
6. LITERATURE 75
ACKNOWLEDGEMENTS 95
CURRICULUM VITAE 96
APPENDIX 98_
10.1 Mouse Cell Surface CD Antigens 98
10.2 Non-CD Murine Cell Surface Antigens 104
II
|
| adam_txt |
TABLE OF CONTENTS
LIST OF ABBREVIATIONS PAGE III
1. INTRODUCTION l_
1.1 Hematopoiesis 1
1.1.1 Hematopoietic Stem Cells (HSC) 1
1.1.2 Hematopoietic Development 3
1.1.3 The Stem Cell Niche 10
1.2 The Receptor Tyrosine Kinase Kit 14
1.3 Stem Cell Mobilization 17
1.4 Background and Objective of this Thesis 19
2. MATERIAL AND METHODS 20_
2.1 Material 20
2.1.1 Buffers and Solutions 20
2.1.2 Enzymes 21
2.1.3 Growth Factors 21
2.1.4 Antibodies and Second Steps 21
2.1.5 Oligonucleotides 21
2.1.6 Cell Culture Media and Supplements 22
2.1.7 Chemicals 22
2.1.8 Plastic Ware and Other Consumables 23
2.2 Methods 24
2.2.1 Mouse Strains and Breeding 24
2.2.2 Treatment of Mice with Cyclophosphamide (Endoxan) 26
2.2.3 Treatment of Mice with rhG-CSF (Pegfilgrastim) 26
2.2.4 Preparation of Organs and Cell Suspensions 27
2.2.5 Determination of White Blood Cell (WBC) Numbers 27
2.2.6 Sorting Non-Erythroid Cells 27
2.2.7 Methylcellulose Assays 28
2.2.8 Phenotypic Analysis by Flow Cytometry 29
2.2.9 Cytospins and Histological Staining 31
2.2.10 Statistical Analysis 31
3. RESULTS 32_
3.1 Treatment with Cyclophosphamide (Endoxan) 32
3.1.1 Mobilization of HSC by Cyclophosphamide 3 3
3.1.2 Mobilization of HPC by Cyclophosphamide 41
3.1.2 Mobilization of Neutrophils by Cyclophosphamide 45
I
3.2 Treatment with rhG-CSF (Pegfilgrastim) 51
3.2.1 Mobilization of HSC by rhG-CSF 52
3.2.2 Mobilization of HPC by rhG-CSF 56
3.2.3 Mobilization of Neutrophils by rhG-CSF 59
4. DISCUSSION 66_
4.1 Mobilization of HSC and HPC 67
4.2 Mobilization of Neutrophil Granulocytes 70
4.3 CY- versus rhG-CSF-mediated Mobilization 71
5. SUMMARY 74
6. LITERATURE 75
ACKNOWLEDGEMENTS 95
CURRICULUM VITAE 96
APPENDIX 98_
10.1 Mouse Cell Surface CD Antigens 98
10.2 Non-CD Murine Cell Surface Antigens 104
II |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Bartels, Susanne 1980- |
| author_GND | (DE-588)134137817 |
| author_facet | Bartels, Susanne 1980- |
| author_role | aut |
| author_sort | Bartels, Susanne 1980- |
| author_variant | s b sb |
| building | Verbundindex |
| bvnumber | BV023400911 |
| ctrlnum | (OCoLC)244072972 (DE-599)BSZ277504996 |
| dewey-full | 573.1554 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 573 - Specific physiological systems in animals |
| dewey-raw | 573.1554 |
| dewey-search | 573.1554 |
| dewey-sort | 3573.1554 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Medizin |
| discipline_str_mv | Biologie Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01147nam a2200301 c 4500</leader><controlfield tag="001">BV023400911</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20090210 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">080717s2007 ad|| m||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)244072972</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BSZ277504996</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">573.1554</subfield><subfield code="2">22/ger</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Bartels, Susanne</subfield><subfield code="d">1980-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)134137817</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit</subfield><subfield code="c">vorgelegt von Susanne Bartels</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2007</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">VI, 105 Bl.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Ulm, Univ., Diss., 2008</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016583700&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-016583700</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV023400911 |
| illustrated | Illustrated |
| index_date | 2024-07-02T21:23:44Z |
| indexdate | 2024-07-09T21:17:47Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016583700 |
| oclc_num | 244072972 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | VI, 105 Bl. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| record_format | marc |
| spelling | Bartels, Susanne 1980- Verfasser (DE-588)134137817 aut Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit vorgelegt von Susanne Bartels 2007 VI, 105 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Ulm, Univ., Diss., 2008 (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016583700&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bartels, Susanne 1980- Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit |
| subject_GND | (DE-588)4113937-9 |
| title | Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit |
| title_auth | Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit |
| title_exact_search | Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit |
| title_exact_search_txtP | Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit |
| title_full | Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit vorgelegt von Susanne Bartels |
| title_fullStr | Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit vorgelegt von Susanne Bartels |
| title_full_unstemmed | Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit vorgelegt von Susanne Bartels |
| title_short | Mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit |
| title_sort | mobilization of hematopoietic stem cells in mice deficient for the receptor tyrosine kinase kit |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016583700&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT bartelssusanne mobilizationofhematopoieticstemcellsinmicedeficientforthereceptortyrosinekinasekit |